Next Article in Journal
Crosstalk between Beta-Catenin and Snail in the Induction of Epithelial to Mesenchymal Transition in Hepatocarcinoma: Role of the ERK1/2 Pathway
Previous Article in Journal
Supramolecular Interactions of Terpyridine-Derived Cores of Metallomesogen Precursors
Open AccessArticle

Molecular Cloning, Sequence Characterization and Expression Analysis of a CD63 Homologue from the Coleopteran Beetle, Tenebrio molitor

1
Division of Plant Biotechnology, Institute of Environmentally-Friendly Agriculture (IEFA), College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Korea
2
National Research Laboratory of Defense Proteins, College of Pharmacy, Pusan National University, Jangjeon Dong, Kumjeong Ku, Busan 609-735, Korea
3
Department of Life Science and Biotechnology, College of Natural Sciences, Soonchunhyang University, Asan city 336-745, Korea
4
Division of Applied Entomology, National Academy of Agricultural Science, Rural Development, 61th, Seodun-dong, Gwonseon-gu, Suwon, Gyeonggi-do 441-853, Korea
5
Department of Biological Science and the Research Institute for Basic Sciences, Hoseo University, Asan 336-795, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(10), 20744-20767; https://doi.org/10.3390/ijms141020744
Received: 6 September 2013 / Revised: 27 September 2013 / Accepted: 3 October 2013 / Published: 15 October 2013
(This article belongs to the Section Biochemistry)
CD63, a member of the tetraspanin membrane protein family, plays a pivotal role in cell growth, motility, signal transduction, host-pathogen interactions and cancer. In this work, the cDNA encoding CD63 homologue (TmCD63) was cloned from larvae of a coleopteran beetle, Tenebrio molitor. The cDNA is comprised of an open reading frame of 705 bp, encoding putative protein of 235 amino acid residues. In silico analysis shows that the protein has four putative transmembrane domains and one large extracellular loop. The characteristic “Cys-Cys-Gly” motif and “Cys188” residues are highly conserved in the large extracellular loop. Phylogenetic analysis of TmCD63 revealed that they belong to the insect cluster with 50%–56% identity. Analysis of spatial expression patterns demonstrated that TmCD63 mRNA is mainly expressed in gut and Malphigian tubules of larvae and the testis of the adult. Developmental expression patterns of CD63 mRNA showed that TmCD63 transcripts are detected in late larval, pupal and adult stages. Interestingly, TmCD63 transcripts are upregulated to the maximum level of 4.5 fold, in response to DAP-type peptidoglycan during the first 6 h, although other immune elicitors also caused significant increase to the transcript level at later time-points. These results suggest that CD63 might contribute to T. molitor immune response against various microbial pathogens. View Full-Text
Keywords: molecular cloning; CD63; TM4SF; expression analysis; Tenebrio molitor; immune elicitors molecular cloning; CD63; TM4SF; expression analysis; Tenebrio molitor; immune elicitors
Show Figures

Graphical abstract

MDPI and ACS Style

Patnaik, B.B.; Kang, S.M.; Seo, G.W.; Lee, H.J.; Patnaik, H.H.; Jo, Y.H.; Tindwa, H.; Lee, Y.S.; Lee, B.L.; Kim, N.J.; Bang, I.S.; Han, Y.S. Molecular Cloning, Sequence Characterization and Expression Analysis of a CD63 Homologue from the Coleopteran Beetle, Tenebrio molitor. Int. J. Mol. Sci. 2013, 14, 20744-20767.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop