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Sericin Enhances the Bioperformance of Collagen-Based Matrices Preseeded with Human-Adipose Derived Stem Cells (hADSCs)

1
Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania
2
Collagen Department, Leather and Footwear Research Institute, 93, Ion Minulescu, Bucharest 031215, Romania
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2013, 14(1), 1870-1889; https://doi.org/10.3390/ijms14011870
Received: 22 October 2012 / Revised: 23 December 2012 / Accepted: 25 December 2012 / Published: 16 January 2013
(This article belongs to the Section Biochemistry)
Current clinical strategies for adipose tissue engineering (ATE), including autologous fat implants or the use of synthetic surrogates, not only are failing in the long term, but also can’t face the latest requirements regarding the aesthetic restoration of the resulted imperfections. In this context, modern strategies in current ATE applications are based on the implantation of 3D cell-scaffold bioconstructs, designed for prospective achievement of in situ functional de novo tissue. Thus, in this paper, we reported for the first time the evaluation of a spongious 60% collagen and 40% sericin scaffold preseeded with human adipose-derived stem cells (hADSCs) in terms of biocompatibility and adipogenic potential in vitro. We showed that the addition of the sticky protein sericin in the composition of a classical collagen sponge enhanced the adhesion and also the proliferation rate of the seeded cells, thus improving the biocompatibility of the novel scaffold. In addition, sericin stimulated PPARγ2 overexpression, triggering a subsequent upregulated expression profile of FAS, aP2 and perilipin adipogenic markers. These features, together with the already known sericin stimulatory potential on cellular collagen production, promote collagen-sericin biomatrix as a good candidate for soft tissue reconstruction and wound healing applications. View Full-Text
Keywords: collagen; sericin; hADSC; biocompatibility; adipogenesis; proliferation; porous biomatrix; adipose tissue engineering collagen; sericin; hADSC; biocompatibility; adipogenesis; proliferation; porous biomatrix; adipose tissue engineering
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MDPI and ACS Style

Dinescu, S.; Galateanu, B.; Albu, M.; Cimpean, A.; Dinischiotu, A.; Costache, M. Sericin Enhances the Bioperformance of Collagen-Based Matrices Preseeded with Human-Adipose Derived Stem Cells (hADSCs). Int. J. Mol. Sci. 2013, 14, 1870-1889.

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