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Reconstitution of Membrane Proteins into Model Membranes: Seeking Better Ways to Retain Protein Activities

Department of Biochemistry and Molecular Biology, Monash University, Melbourne 3800, Australia
School of Chemistry, Monash University, Melbourne 3800, Australia
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(1), 1589-1607;
Received: 20 December 2012 / Revised: 9 January 2013 / Accepted: 10 January 2013 / Published: 14 January 2013
(This article belongs to the Special Issue Phospholipids: Molecular Sciences 2012)
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The function of any given biological membrane is determined largely by the specific set of integral membrane proteins embedded in it, and the peripheral membrane proteins attached to the membrane surface. The activity of these proteins, in turn, can be modulated by the phospholipid composition of the membrane. The reconstitution of membrane proteins into a model membrane allows investigation of individual features and activities of a given cell membrane component. However, the activity of membrane proteins is often difficult to sustain following reconstitution, since the composition of the model phospholipid bilayer differs from that of the native cell membrane. This review will discuss the reconstitution of membrane protein activities in four different types of model membrane — monolayers, supported lipid bilayers, liposomes and nanodiscs, comparing their advantages in membrane protein reconstitution. Variation in the surrounding model environments for these four different types of membrane layer can affect the three-dimensional structure of reconstituted proteins and may possibly lead to loss of the proteins activity. We also discuss examples where the same membrane proteins have been successfully reconstituted into two or more model membrane systems with comparison of the observed activity in each system. Understanding of the behavioral changes for proteins in model membrane systems after membrane reconstitution is often a prerequisite to protein research. It is essential to find better solutions for retaining membrane protein activities for measurement and characterization in vitro. View Full-Text
Keywords: nanodiscs; liposomes; supported lipid bilayer; monolayer; membrane protein activity nanodiscs; liposomes; supported lipid bilayer; monolayer; membrane protein activity

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Shen, H.-H.; Lithgow, T.; Martin, L. Reconstitution of Membrane Proteins into Model Membranes: Seeking Better Ways to Retain Protein Activities. Int. J. Mol. Sci. 2013, 14, 1589-1607.

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