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The Role of Protein Crystallography in Defining the Mechanisms of Biogenesis and Catalysis in Copper Amine Oxidase

Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, 6-155 Jackson Hall, 321 Church St. SE, Minneapolis, MN 55455, USA
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Int. J. Mol. Sci. 2012, 13(5), 5375-5405; https://doi.org/10.3390/ijms13055375
Received: 6 April 2012 / Revised: 22 April 2012 / Accepted: 26 April 2012 / Published: 3 May 2012
(This article belongs to the Special Issue Protein Crystallography in Molecular Biology)
Copper amine oxidases (CAOs) are a ubiquitous group of enzymes that catalyze the conversion of primary amines to aldehydes coupled to the reduction of O2 to H2O2. These enzymes utilize a wide range of substrates from methylamine to polypeptides. Changes in CAO activity are correlated with a variety of human diseases, including diabetes mellitus, Alzheimer’s disease, and inflammatory disorders. CAOs contain a cofactor, 2,4,5-trihydroxyphenylalanine quinone (TPQ), that is required for catalytic activity and synthesized through the post-translational modification of a tyrosine residue within the CAO polypeptide. TPQ generation is a self-processing event only requiring the addition of oxygen and Cu(II) to the apoCAO. Thus, the CAO active site supports two very different reactions: TPQ synthesis, and the two electron oxidation of primary amines. Crystal structures are available from bacterial through to human sources, and have given insight into substrate preference, stereospecificity, and structural changes during biogenesis and catalysis. In particular both these processes have been studied in crystallo through the addition of native substrates. These latter studies enable intermediates during physiological turnover to be directly visualized, and demonstrate the power of this relatively recent development in protein crystallography. View Full-Text
Keywords: amine oxidase; copper; biogenesis; catalysis; cofactor; protein crystallography amine oxidase; copper; biogenesis; catalysis; cofactor; protein crystallography
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MDPI and ACS Style

Klema, V.J.; Wilmot, C.M. The Role of Protein Crystallography in Defining the Mechanisms of Biogenesis and Catalysis in Copper Amine Oxidase. Int. J. Mol. Sci. 2012, 13, 5375-5405. https://doi.org/10.3390/ijms13055375

AMA Style

Klema VJ, Wilmot CM. The Role of Protein Crystallography in Defining the Mechanisms of Biogenesis and Catalysis in Copper Amine Oxidase. International Journal of Molecular Sciences. 2012; 13(5):5375-5405. https://doi.org/10.3390/ijms13055375

Chicago/Turabian Style

Klema, Valerie J.; Wilmot, Carrie M. 2012. "The Role of Protein Crystallography in Defining the Mechanisms of Biogenesis and Catalysis in Copper Amine Oxidase" Int. J. Mol. Sci. 13, no. 5: 5375-5405. https://doi.org/10.3390/ijms13055375

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