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Types, Causes, Detection and Repair of DNA Fragmentation in Animal and Human Sperm Cells

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Sexing Technologies, 22575 State Highway 6 South, Navasota, TX 77868, USA
2
Biology Department, Universidad Autonoma of Madrid, C/ Darwin nº 2. 28049 Madrid, Spain
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Int. J. Mol. Sci. 2012, 13(11), 14026-14052; https://doi.org/10.3390/ijms131114026
Received: 31 July 2012 / Revised: 16 October 2012 / Accepted: 18 October 2012 / Published: 31 October 2012
(This article belongs to the Section Biochemistry)
Concentration, motility and morphology are parameters commonly used to determine the fertilization potential of an ejaculate. These parameters give a general view on the quality of sperm but do not provide information about one of the most important components of the reproductive outcome: DNA. Either single or double DNA strand breaks can set the difference between fertile and infertile males. Sperm DNA fragmentation can be caused by intrinsic factors like abortive apoptosis, deficiencies in recombination, protamine imbalances or oxidative stress. Damage can also occur due to extrinsic factors such as storage temperatures, extenders, handling conditions, time after ejaculation, infections and reaction to medicines or post-testicular oxidative stress, among others. Two singular characteristics differentiate sperm from somatic cells: Protamination and absence of DNA repair. DNA repair in sperm is terminated as transcription and translation stops post-spermiogenesis, so these cells have no mechanism to repair the damage occurred during their transit through the epididymis and post-ejaculation. Oocytes and early embryos have been shown to repair sperm DNA damage, so the effect of sperm DNA fragmentation depends on the combined effects of sperm chromatin damage and the capacity of the oocyte to repair it. In this contribution we review some of these issues. View Full-Text
Keywords: sperm; infertility; DNA fragmentation; DNA repair; oocyte sperm; infertility; DNA fragmentation; DNA repair; oocyte
MDPI and ACS Style

González-Marín, C.; Gosálvez, J.; Roy, R. Types, Causes, Detection and Repair of DNA Fragmentation in Animal and Human Sperm Cells. Int. J. Mol. Sci. 2012, 13, 14026-14052.

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