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Open AccessArticle

Determination of Phenolic Acids and Flavonoids in Taraxacum formosanum Kitam by Liquid Chromatography-Tandem Mass Spectrometry Coupled with a Post-Column Derivatization Technique

1
Department of Food Science, Fu Jen University, Taipei 242, Taiwan
2
Graduate Institute of Medicine, Fu Jen University, Taipei 242, Taiwan
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2012, 13(1), 260-285; https://doi.org/10.3390/ijms13010260
Received: 28 October 2011 / Revised: 25 November 2011 / Accepted: 12 December 2011 / Published: 27 December 2011
(This article belongs to the Section Biochemistry)
A liquid chromatography-tandem mass spectrometry method (LC-MS/MS) was developed for the determination of phenolic acids and flavonoids in a medicinal Chinese herb Taraxacum formosanum Kitam. Initially, both phenolic acids and flavonoids were extracted with 50% ethanol in a water-bath at 60 °C for 3 h and eventually separated into acidic fraction and neutral fraction by using a C18 cartridge. A total of 29 compounds were separated within 68 min by employing a Gemini C18 column and a gradient solvent system of 0.1% formic acid and acetonitrile at a flow rate of 1.0 mL/min. Based on the retention behavior as well as absorption and mass spectra, 19 phenolic acids and 10 flavonoids were identified and quantified in T. formosanum, with the former ranging from 14.1 μg/g to 10,870.4 μg/g, and the latter from 9.9 μg/g to 325.8 μg/g. For further identification of flavonoids, a post-column derivatization method involving shift reagents such as sodium acetate or aluminum chloride was used and the absorption spectral characteristics without or with shift reagents were compared. An internal standard syringic acid was used for quantitation of phenolic acids, whereas (±) naringenin was found suitable for quantitation of flavonoids. The developed LC-MS/MS method showed high reproducibility, as evident from the relative standard deviation (RSD) values for intra-day and inter-day variability being 1.0–6.8% and 2.0–7.7% for phenolic acids and 3.7–7.4% and 1.5–8.1% for flavonoids, respectively, and thus may be applied for simultaneous determination of phenolic acids and flavonoids in Chinese herb and nutraceuticals. View Full-Text
Keywords: Taraxacum formosanum Kitam; phenolic acid; flavonoid; LC-MS-MS Taraxacum formosanum Kitam; phenolic acid; flavonoid; LC-MS-MS
MDPI and ACS Style

Chen, H.-J.; Inbaraj, B.S.; Chen, B.-H. Determination of Phenolic Acids and Flavonoids in Taraxacum formosanum Kitam by Liquid Chromatography-Tandem Mass Spectrometry Coupled with a Post-Column Derivatization Technique. Int. J. Mol. Sci. 2012, 13, 260-285.

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