Effect of a Steaming Treatment on the Alpha-Glucosidase Inhibitory Components in the Brown Alga Sargassum fusiforme
Abstract
1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. Sample Collection and Preparation
2.3. Preparation of the Crude Extract
2.4. α-Glucosidase Inhibition Assay
2.5. α-Amylase Inhibition Assay
2.6. Total Polyphenol Content
2.7. Fucoxanthin and Chlorophyll Derivative Contents
2.8. Free Fatty Acid Composition Analysis
2.9. α-Glucosidase Inhibitory Profiling of SF
2.10. Analysis of the Components with Inhibitory Activity in the Fractions
2.11. Random Forest Analysis of Fatty Acids
2.12. Molecular Docking
2.13. Statistical Analysis
3. Results
3.1. Effect of the Extraction Solvent on Carbohydrate-Digesting Enzyme Inhibition by SF
3.2. Effect of the Extraction Solvent on the Phytochemical Compositions and Free Fatty Acids in SF
3.3. Effect of Steaming on α-Glucosidase Inhibition and the Yield of SF
3.4. Effect of Steaming on the α-Glucosidase Inhibitory Profile of SF
3.5. Identification of the Active Components of Frac. 43
3.6. Free Fatty Acid Determination and Variable Importance Evaluation
3.7. Molecular Docking of Alpha-Glucosidase and Fatty Acids
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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SF-0h | SF-1h | SF-2h | SF-4h | |
---|---|---|---|---|
Total polyphenols $ | 419.96 ± 13.05 a | 131.42 ± 5.14 b | 110.97 ± 4.30 c | 114.79 ± 1.72 c |
Fucoxanthin | 9.60 ± 0.74 b | 12.17 ± 0.57 a | 8.39 ± 1.10 b | 3.75 ± 0.73 c |
Chlorophyll c | 11.86 ± 0.30 | - | - | - |
Chlorophyll a | 55.30 ± 4.56 a | 3.70 ± 2.05 b | - | - |
Chlorophyll a′ | 2.80 ± 0.60 | - | - | - |
Pheophytin a | 24.15 ± 2.85 d | 132.62 ± 4.48 a | 101.88 ± 1.39 b | 58.16 ± 8.23 c |
Pheophytin a′ | 7.63 ± 0.77 d | 37.30 ± 1.78 a | 27.43 ± 2.55 b | 18.00 ± 1.78 c |
Pyropheophytin a | - | 45.25 ± 1.98 c | 81.64 ± 2.44 b | 122.08 ± 17.84 a |
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Liu, X.; Gu, Y.; Zhou, Y.; Zhang, R.; Koyama, T. Effect of a Steaming Treatment on the Alpha-Glucosidase Inhibitory Components in the Brown Alga Sargassum fusiforme. Molecules 2024, 29, 6000. https://doi.org/10.3390/molecules29246000
Liu X, Gu Y, Zhou Y, Zhang R, Koyama T. Effect of a Steaming Treatment on the Alpha-Glucosidase Inhibitory Components in the Brown Alga Sargassum fusiforme. Molecules. 2024; 29(24):6000. https://doi.org/10.3390/molecules29246000
Chicago/Turabian StyleLiu, Xinxin, Yipeng Gu, Yihao Zhou, Ruiqi Zhang, and Tomoyuki Koyama. 2024. "Effect of a Steaming Treatment on the Alpha-Glucosidase Inhibitory Components in the Brown Alga Sargassum fusiforme" Molecules 29, no. 24: 6000. https://doi.org/10.3390/molecules29246000
APA StyleLiu, X., Gu, Y., Zhou, Y., Zhang, R., & Koyama, T. (2024). Effect of a Steaming Treatment on the Alpha-Glucosidase Inhibitory Components in the Brown Alga Sargassum fusiforme. Molecules, 29(24), 6000. https://doi.org/10.3390/molecules29246000