Precise Molecular Engineering of Type I Photosensitizer with Aggregation-Induced Emission for Image-Guided Photodynamic Eradication of Biofilm
Abstract
:1. Introduction
2. Result and Discussion
2.1. Molecular Design and Synthesis
2.2. Photophysical Properties of TTTDM and F-127 AIE Nanoparticles
2.3. ROS Generation Ability of F-127 AIE Nanoparticles
2.4. Bacteria and Biofilm Imaging
2.5. Anti-Biofilm Treatment
3. Conclusions
4. Experimental Sessions
4.1. Materials
4.2. Instrument and Characterization
4.3. Synthesis and Purification
4.3.1. Synthesis of 1
4.3.2. Synthesis of 2
4.3.3. Synthesis of 3
4.3.4. Synthesis of 4
4.4. Preparation of F-127 AIE Nanoparticles
4.5. Detection of ROS Generation in Aqueous Solution
4.6. Biofilm Culture
4.7. Biofilm Dispersion Assay
4.8. Fluorescence Imaging
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Shi, J.; Wang, Y.; He, W.; Ye, Z.; Liu, M.; Zhao, Z.; Lam, J.W.Y.; Zhang, P.; Kwok, R.T.K.; Tang, B.Z. Precise Molecular Engineering of Type I Photosensitizer with Aggregation-Induced Emission for Image-Guided Photodynamic Eradication of Biofilm. Molecules 2023, 28, 5368. https://doi.org/10.3390/molecules28145368
Shi J, Wang Y, He W, Ye Z, Liu M, Zhao Z, Lam JWY, Zhang P, Kwok RTK, Tang BZ. Precise Molecular Engineering of Type I Photosensitizer with Aggregation-Induced Emission for Image-Guided Photodynamic Eradication of Biofilm. Molecules. 2023; 28(14):5368. https://doi.org/10.3390/molecules28145368
Chicago/Turabian StyleShi, Jinghong, Yucheng Wang, Wei He, Ziyue Ye, Mengli Liu, Zheng Zhao, Jacky Wing Yip Lam, Pengfei Zhang, Ryan Tsz Kin Kwok, and Ben Zhong Tang. 2023. "Precise Molecular Engineering of Type I Photosensitizer with Aggregation-Induced Emission for Image-Guided Photodynamic Eradication of Biofilm" Molecules 28, no. 14: 5368. https://doi.org/10.3390/molecules28145368