Knockout of KLF10 Ameliorated Diabetic Renal Fibrosis via Downregulation of DKK-1
Abstract
:1. Introduction
2. Results
2.1. Biochemical Properties of Experimental Animals
2.2. Knockout of KLF10 and Its Downstream Targets in Diabetic Mice
2.3. Effects of KLF10 Knockout on the Expression of Fibrosis-Related Genes
3. Discussion
4. Materials and Methods
4.1. Animal Model
4.2. Immunohistochemical (IHC) and Masson’s Trichrome Staining
4.3. Laser Capture Microdissection
4.4. Quantitative Reverse Transcription–Polymerase Chain Reaction
4.5. Western Blot Analysis
4.6. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Control | DM | KLF10-KO | KLF10-KO + DM | |
---|---|---|---|---|
Fasting blood glucose (mg/dL) | 156 ± 21 | 487 ± 70 * | 153 ± 17 | 496 ± 56 * |
HbA1C (%) | 4.1 ± 0.9 | 8.1 ± 0.9 * | 4.3 ± 1.2 | 8.4 ± 0.3 * |
Body weight (BW, g) | 29.1 ± 1.4 | 24.1 ± 0.7 * | 33.0 ± 1.9 | 23.9 ± 1.2 * |
Kidney/BW (%) | 0.76 ± 0.21 | 1.10 ± 0.21 * | 0.82 ± 0.23 | 0.89 ± 0.07 *# |
Urine (TP/CRE) | 5.9 ± 0.7 | 11.2 ± 1.2 | 6.1 ± 0.4 | 8.4 ± 0.7 *# |
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Hsu, Y.-C.; Ho, C.; Shih, Y.-H.; Ni, W.-C.; Li, Y.-C.; Chang, H.-C.; Lin, C.-L. Knockout of KLF10 Ameliorated Diabetic Renal Fibrosis via Downregulation of DKK-1. Molecules 2022, 27, 2644. https://doi.org/10.3390/molecules27092644
Hsu Y-C, Ho C, Shih Y-H, Ni W-C, Li Y-C, Chang H-C, Lin C-L. Knockout of KLF10 Ameliorated Diabetic Renal Fibrosis via Downregulation of DKK-1. Molecules. 2022; 27(9):2644. https://doi.org/10.3390/molecules27092644
Chicago/Turabian StyleHsu, Yung-Chien, Cheng Ho, Ya-Hsueh Shih, Wen-Chiu Ni, Yi-Chen Li, Hsiu-Ching Chang, and Chun-Liang Lin. 2022. "Knockout of KLF10 Ameliorated Diabetic Renal Fibrosis via Downregulation of DKK-1" Molecules 27, no. 9: 2644. https://doi.org/10.3390/molecules27092644