The Alleviating Effect of Lagerstroemia indica Flower Extract on Stretch Marks through Regulation of Mast Cells
Abstract
:1. Introduction
2. Results
2.1. LIFE Inhibits Mast Cell Binding to FN
2.2. LIFE Decreases the Expression of Integrin α4 and β3
2.3. LIFE Inhibits FAK Phosphorylation but Not Intact FAK Protein
2.4. LIFE Decrease the IgE-Induced Relases of Graunles and Cytokine IL-3 on FN-Coated Plate
2.5. Conditioned Medium from Activated RBL-2H3 Changes the Expression of ECM Components and Inflammatory Mediators in Fibroblast
2.6. LIFE Restores Abnormal Genes Expression in Fibroblast in Response to the Conditioned Medium from the Activated Mast Cells
2.7. Phenolic Compounds Analysis of LIFE
2.8. Ellagic Acid Inhibits Mast Cell Binding to FN
3. Discussion
4. Materials and Method
4.1. Preparation of LIF Extracts
4.2. Cell Culture and Cell Treatment
4.3. Adhesion Assay
4.4. β-Hexosaminidase Release Assay
4.5. Enzyme-Linked Immunosorbent Assay
4.6. RNA Isolation and Quantitative Real-Time RT–PCR
4.7. Flow Cytometry Analysis
4.8. Western Blotting
4.9. Preparation of Activated Mast Cell-CM and Cell Treatment
4.10. High-Performance Liquid Chromatography (HPLC)
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
List of Abbreviations
Abbreviations | Full Name |
DNP-BSA | 2,4-dinitrophenylated BSA |
ACT | actin |
α-SMA | alpha-smooth muscle actin |
RGD | arginine-glycine-aspartic acid |
BGN | biglycan |
BSA | bovine serum albumin |
CXCL8 | C-X-C motif chemokine ligand 8 |
CD26 | cluster of differentiation 26 |
CD74 | cluster of differentiation 74 |
COL1 | collagen type I |
COL3 | collagens type III |
CM | conditioned medium |
CS | connecting segment |
DMEM | Dulbecco’s modified Eagle’s medium |
EMEM | eagle’s minimum essential medium |
Echi | echistatin |
ELN | elastin |
ELISA | enzyme-linked immunosorbent assay |
ECM | extracellular matrix |
FceR1 | Fc epsilonR1 alpha (FceR1) |
FBN | fibrillin |
FN | fibronectin |
FAK | focal adhesion kinase |
HBSS | hanks balanced salt solution |
IgE | immunoglobulin E |
IL-3 | interleukin-3 |
LI | Lagerstroemia indica |
LIF | Lagerstroemia indica flower |
LIFE | Lagerstroemia indica flower extract |
LUM | lumican |
LOX | lysyl oxidase |
MMP1 | matrix metallopeptidase 1 |
MMP3 | matrix metallopeptidase 3 |
NHDF | normal human dermal fibroblast |
PBS | phosphaste-buffered saline |
RBL | rat basophilic leukemia |
SA | striae albae |
SD | striae distensae |
SR | striae rubrae |
TGF-β | transforming growth factor-β |
TNF-α | tumor necrosis factor-α |
VEGF-A | vascular endothelial growth factor A |
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Gene Name | Accession Number | Primer Sequence |
---|---|---|
Integrin α4 | NM_001107737.1 | F: ctccccacaggcctttattt |
R: tctctgtcacgtcgcagttt | ||
Integrin α5 | NM_001108118.1 | F: agctgcatttccgagtctg |
R: ctcacactgaaggctgaacg | ||
Integrin β3 | NM_153720.1 | F: cacctgcatgtccaccaa |
R: cagctgccacactcacagtt |
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Yeom, M.; Ji, H.; Shin, J.; Cho, E.; Ryu, D.-H.; Park, D.; Jung, E. The Alleviating Effect of Lagerstroemia indica Flower Extract on Stretch Marks through Regulation of Mast Cells. Molecules 2022, 27, 1274. https://doi.org/10.3390/molecules27041274
Yeom M, Ji H, Shin J, Cho E, Ryu D-H, Park D, Jung E. The Alleviating Effect of Lagerstroemia indica Flower Extract on Stretch Marks through Regulation of Mast Cells. Molecules. 2022; 27(4):1274. https://doi.org/10.3390/molecules27041274
Chicago/Turabian StyleYeom, Miji, Hyanggi Ji, Jongheon Shin, Eunae Cho, De-Hun Ryu, Deokhoon Park, and Eunsun Jung. 2022. "The Alleviating Effect of Lagerstroemia indica Flower Extract on Stretch Marks through Regulation of Mast Cells" Molecules 27, no. 4: 1274. https://doi.org/10.3390/molecules27041274