Methodological Fallacies in the Determination of Serum/Plasma Glutathione Limit Its Translational Potential in Chronic Obstructive Pulmonary Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Search Strategy and Studies Selection
2.2. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Control Group | COPD Group | |||||||
---|---|---|---|---|---|---|---|---|
First Author, Year, and Country | Assay Type | Derivatization Reagent | GSH Form | Measure Units | n | GSH Mean ± SD | n | GSH Mean ± SD |
Andersson A et al., 2001, Sweden [28] | HPLC | dithiopyridine | t | μmol/L | 29 | 5.69 ± 1.33 | 19 | 4.93 ± 1.43 |
Flaring UB et al., 2005, Sweden [29] | HPLC | monobromobimane | t | μmol/L | 10 | 5.9 ± 1.03 | 21 | 4.6 ± 0.95 |
Liu SF et al., 2015, Taiwan [30] | Spectr | DTNB | t | mU/mL | 110 | 6.8 ± 1.3 | 86 | 4.5 ± 1.3 |
Zinellu A et al., 2016, Italy [31] | CE | 5-IAF | t | μmol/L | 29 | 7.2 ± 2.6 | 29 | 6.7 ± 2.4 |
Zinellu A et al., 2020, Italy [32] | CE | 5-IAF | t | μmol/L | 54 | 7.5 ± 2.9 | 54 | 7.4 ± 3.0 |
Premanand et al., 2007, India [33] | Spectr | DTNB | r | μmol/ mg of protein | 20 | 4.85 ± 0.97 | 20 | 5.72 ± 1.02 |
Andersson A et al., 2001, Sweden [28] | HPLC | dithiopyridine | r | μmol/L | 29 | 1.58 ± 0.53 | 19 | 1.23 ± 0.26 |
Vibhuti A et al., 2007, India [34] | Spectr | DTNB | r | μmol/L | 136 | 4.10 ± 2.64 | 202 | 3.06 ± 1.45 |
Nicks ME et al., 2011, USA [35] | Spectr | M2VP | r | μmol/L | 136 | 0.53 ± 0.34 | 367 | 0.50 ± 0.42 |
Rodriguez DA et al., 2012, Spain [36] | Kit | NR | r | μmol/L | 12 | 0.47 ± 0.04 | 18 | 0.49 ± 0.05 |
Zeng M et al., 2013, China [37] | Kit | NR | r | mg/L (μmol/L) | 14 | 413 ± 8 # (1.340 ± 30) | 35 | 352 ± 44 # (1.147 ± 143) |
Ben Anes AI et al., 2014, Tunisia [38] | Spectr | DTNB | r | μmol/L | 229 | 96 ± 58 | 153 | 47 ± 26 |
Ismail Md et al., 2015, Bangladesh [39] | Spectr | DTNB | r | μmol/L | 20 | 415 ± 20 | 30 | 279 ± 23 |
Cheng L et al., 2016, China [40] | Spectr | DTNB | r | mg/L (μmol/L) | 64 | 17.2 ± 1.2 # (56 ± 4) | 58 | 10.6 ± 0.8 # (35 ± 3) |
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Sotgia, S.; Fois, A.G.; Paliogiannis, P.; Carru, C.; Mangoni, A.A.; Zinellu, A. Methodological Fallacies in the Determination of Serum/Plasma Glutathione Limit Its Translational Potential in Chronic Obstructive Pulmonary Disease. Molecules 2021, 26, 1572. https://doi.org/10.3390/molecules26061572
Sotgia S, Fois AG, Paliogiannis P, Carru C, Mangoni AA, Zinellu A. Methodological Fallacies in the Determination of Serum/Plasma Glutathione Limit Its Translational Potential in Chronic Obstructive Pulmonary Disease. Molecules. 2021; 26(6):1572. https://doi.org/10.3390/molecules26061572
Chicago/Turabian StyleSotgia, Salvatore, Alessandro G. Fois, Panagiotis Paliogiannis, Ciriaco Carru, Arduino A. Mangoni, and Angelo Zinellu. 2021. "Methodological Fallacies in the Determination of Serum/Plasma Glutathione Limit Its Translational Potential in Chronic Obstructive Pulmonary Disease" Molecules 26, no. 6: 1572. https://doi.org/10.3390/molecules26061572