A 60% Edible Ethanolic Extract of Ulmus davidiana Inhibits Vascular Endothelial Growth Factor-Induced Angiogenesis
Abstract
:1. Introduction
2. Results
2.1. U60E Reduces NO Production by Suppressing eNOS Activity in Human Umbilical Vein Endothelial Cells (HUVECs)
2.2. U60E Inhibits VEGF-Induced Angiogenesis in HUVECs
2.3. U60E Inhibits Angiogenesis by Suppressing VEGF-Mediated eNOS Activity
2.4. U60E Inhibits eNOS Activity and NO Production by Reducing Akt Activity
3. Discussion
4. Materials and Methods
4.1. Cell Cultures
4.2. Reagents and Antibodies
4.3. Preparation of U60E Extracts
4.4. Cell Viability Assay
4.5. Western Blot Analysis
4.6. BrdU Enzyme-Linked Immunosorbent Assay (ELISA) for Cell Proliferation Estimation
4.7. Cell Counting
4.8. Tube Formation
4.9. Transwell Cell Migration Assay
4.10. NO Production Measurement
4.11. Statistics Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Park, J.; Kim, H.-O.; Park, K.-H.; Wie, M.-B.; Choi, S.-E.; Yun, J.-H. A 60% Edible Ethanolic Extract of Ulmus davidiana Inhibits Vascular Endothelial Growth Factor-Induced Angiogenesis. Molecules 2021, 26, 781. https://doi.org/10.3390/molecules26040781
Park J, Kim H-O, Park K-H, Wie M-B, Choi S-E, Yun J-H. A 60% Edible Ethanolic Extract of Ulmus davidiana Inhibits Vascular Endothelial Growth Factor-Induced Angiogenesis. Molecules. 2021; 26(4):781. https://doi.org/10.3390/molecules26040781
Chicago/Turabian StylePark, Jeongho, Hyun-Ouk Kim, Kwang-Hyun Park, Myung-Bok Wie, Sun-Eun Choi, and Jang-Hyuk Yun. 2021. "A 60% Edible Ethanolic Extract of Ulmus davidiana Inhibits Vascular Endothelial Growth Factor-Induced Angiogenesis" Molecules 26, no. 4: 781. https://doi.org/10.3390/molecules26040781