Anti-Oxidant and Anti-Inflammatory Effects of Lipopolysaccharide from Rhodobacter sphaeroides against Ethanol-Induced Liver and Kidney Toxicity in Experimental Rats
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of LPS-RS on Serum Levels of Liver Enzymes and Kidney Function Markers
2.2. Effect of LPS-RS on Oxidative Stress and Inflammatory Markers in the Liver Tissue
2.3. Effect of LPS-RS on the Expression of Hepcidin in the Liver Tissue and Serum Levels of Iron and Ferritin
2.4. Effect of LPS-RS on Markers of Inflammation and Kidney Injury in the Renal Tissue
2.5. Histopathological Examination of the Liver and Kidney Tissues
3. Materials and Methods
3.1. Experimental Animals
3.2. Study Design
3.3. Collection of Samples
3.4. Determination of Liver and Kidney Function Markers in Serum
3.5. Assessment of Markers of Oxidative Stress and Inflammation in the Liver Tissue
3.6. Quantitative Assessment of the Expression of Nuclear Factor Kappa B (NF-κB) and Hepcidin by Quantitative Real-Time PCR
3.7. Determination of Markers of Renal Inflammation and Injury in the Kidney Tissue
3.8. Histopathological Examination of the Liver and the Kidney
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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ALT (U\L) | AST (U\L) | ALP (U\L) | BUN (mg\dL) | Creatinine (mg\dL) | |
---|---|---|---|---|---|
Intact control | 42.0 ± 4.7 | 51.8 ± 5.3 | 53.0 ± 6.9 | 19.6 ± 2.1 | 0.9 ± 0.2 |
LPS-RS (800 μg/kg) | 45.5 ± 5.2 | 57.7 ± 6.9 | 55.9 ± 6.6 | 21.0 ± 2.5 | 1.1 ± 0.2 |
Ethanol (3 g/kg) | 105.0 ± 15.5 ab | 225.6 ± 22.2 ab | 185.0 ± 19.1 ab | 27.9 ± 1.9 ab | 1.6 ± 0.3 ab |
LPS-RS + ethanol (3 g/kg) | 55.0 ± 6.0 abc | 92.3 ± 9.5 abc | 56.6 ± 6.0 c | 26.6 ± 2.4 ab | 1.1 ± 0.3 c |
Ethanol (5 g/kg) | 162.4 ± 17.1 abcd | 265 ± 27.7 abcd | 248.7 ± 25.8 abcd | 28.3 ± 2.1 ab | 1.9 ± 0.2 abd |
LPS-RS + ethanol (5 g/kg) | 99.0 ± 11.2 abde | 204.4 ± 21.9 abde | 115.6 ± 12.6 abcde | 26.7 ± 1.3 ab | 1.4 ± 0.3 abe |
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Mehanna, E.T.; Ali, A.-S.A.; El-Shaarawy, F.; Mesbah, N.M.; Abo-Elmatty, D.M.; Aborehab, N.M. Anti-Oxidant and Anti-Inflammatory Effects of Lipopolysaccharide from Rhodobacter sphaeroides against Ethanol-Induced Liver and Kidney Toxicity in Experimental Rats. Molecules 2021, 26, 7437. https://doi.org/10.3390/molecules26247437
Mehanna ET, Ali A-SA, El-Shaarawy F, Mesbah NM, Abo-Elmatty DM, Aborehab NM. Anti-Oxidant and Anti-Inflammatory Effects of Lipopolysaccharide from Rhodobacter sphaeroides against Ethanol-Induced Liver and Kidney Toxicity in Experimental Rats. Molecules. 2021; 26(24):7437. https://doi.org/10.3390/molecules26247437
Chicago/Turabian StyleMehanna, Eman T., Al-Shimaa A. Ali, Fatma El-Shaarawy, Noha M. Mesbah, Dina M. Abo-Elmatty, and Nora M. Aborehab. 2021. "Anti-Oxidant and Anti-Inflammatory Effects of Lipopolysaccharide from Rhodobacter sphaeroides against Ethanol-Induced Liver and Kidney Toxicity in Experimental Rats" Molecules 26, no. 24: 7437. https://doi.org/10.3390/molecules26247437