STL1, a New AKT Inhibitor, Synergizes with Flavonoid Quercetin in Enhancing Cell Death in A Chronic Lymphocytic Leukemia Cell Line
Abstract
:1. Introduction
2. Results
2.1. Quercetin Enhances Cell Death Induced by STL1 in HG-3 Cells
2.2. Quercetin Enhances STL1 Sensitivity in HG-3 through AKT Inhibition
2.3. Quercetin and STL1 Co-Treatment Induce Apoptosis in HG-3 Cells
2.4. Quercetin and STL1 Co-Treatment Induces Autophagy in HG-3 Cells
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Culture and Treatment
4.3. Cell Viability Assay and Combination Index Determination
4.4. Apoptosis Assays
4.5. Autophagy Assays
4.6. Immunoblotting
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Cervellera, C.; Russo, M.; Dotolo, S.; Facchiano, A.; Russo, G.L. STL1, a New AKT Inhibitor, Synergizes with Flavonoid Quercetin in Enhancing Cell Death in A Chronic Lymphocytic Leukemia Cell Line. Molecules 2021, 26, 5810. https://doi.org/10.3390/molecules26195810
Cervellera C, Russo M, Dotolo S, Facchiano A, Russo GL. STL1, a New AKT Inhibitor, Synergizes with Flavonoid Quercetin in Enhancing Cell Death in A Chronic Lymphocytic Leukemia Cell Line. Molecules. 2021; 26(19):5810. https://doi.org/10.3390/molecules26195810
Chicago/Turabian StyleCervellera, Carmen, Maria Russo, Serena Dotolo, Angelo Facchiano, and Gian Luigi Russo. 2021. "STL1, a New AKT Inhibitor, Synergizes with Flavonoid Quercetin in Enhancing Cell Death in A Chronic Lymphocytic Leukemia Cell Line" Molecules 26, no. 19: 5810. https://doi.org/10.3390/molecules26195810