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Molecules
Volume 26
Issue 17
10.3390/molecules26175115
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Article

Gadolinium-Based Paramagnetic Relaxation Enhancement Agent Enhances Sensitivity for NUS Multidimensional NMR-Based Metabolomics

by
Chandrashekhar Honrao
1,
Nathalie Teissier
1,
Bo Zhang
1,
Robert Powers
2,3,* and
Elizabeth M. O’Day
1,*
1
Olaris, Inc., Waltham, MA 02451, USA
2
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
3
Nebraska Center for Integrated Biomolecular Communication, University of Nebraska-Lincoln, Lincoln, NE 68588, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Robert Brinson
Molecules 2021, 26(17), 5115; https://doi.org/10.3390/molecules26175115
Received: 22 July 2021 / Revised: 16 August 2021 / Accepted: 20 August 2021 / Published: 24 August 2021
(This article belongs to the Special Issue Practical Applications of NMR to Solve Real-World Problems)
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Abstract

Gadolinium is a paramagnetic relaxation enhancement (PRE) agent that accelerates the relaxation of metabolite nuclei. In this study, we noted the ability of gadolinium to improve the sensitivity of two-dimensional, non-uniform sampled NMR spectral data collected from metabolomics samples. In time-equivalent experiments, the addition of gadolinium increased the mean signal intensity measurement and the signal-to-noise ratio for metabolite resonances in both standard and plasma samples. Gadolinium led to highly linear intensity measurements that correlated with metabolite concentrations. In the presence of gadolinium, we were able to detect a broad array of metabolites with a lower limit of detection and quantification in the low micromolar range. We also observed an increase in the repeatability of intensity measurements upon the addition of gadolinium. The results of this study suggest that the addition of a gadolinium-based PRE agent to metabolite samples can improve NMR-based metabolomics. View Full-Text
Keywords: NMR; metabolomics; paramagnetic; relaxation; gadolinium NMR; metabolomics; paramagnetic; relaxation; gadolinium
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MDPI and ACS Style

Honrao, C.; Teissier, N.; Zhang, B.; Powers, R.; O’Day, E.M. Gadolinium-Based Paramagnetic Relaxation Enhancement Agent Enhances Sensitivity for NUS Multidimensional NMR-Based Metabolomics. Molecules 2021, 26, 5115. https://doi.org/10.3390/molecules26175115

AMA Style

Honrao C, Teissier N, Zhang B, Powers R, O’Day EM. Gadolinium-Based Paramagnetic Relaxation Enhancement Agent Enhances Sensitivity for NUS Multidimensional NMR-Based Metabolomics. Molecules. 2021; 26(17):5115. https://doi.org/10.3390/molecules26175115

Chicago/Turabian Style

Honrao, Chandrashekhar, Nathalie Teissier, Bo Zhang, Robert Powers, and Elizabeth M. O’Day. 2021. "Gadolinium-Based Paramagnetic Relaxation Enhancement Agent Enhances Sensitivity for NUS Multidimensional NMR-Based Metabolomics" Molecules 26, no. 17: 5115. https://doi.org/10.3390/molecules26175115

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