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Article

In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe

1
Medical Device Research Institute, College of Science and Engineering, Flinders University, Adelaide, SA 5042, Australia
2
Flinders Institute for Nanoscale Science and Technology, College of Science and Engineering, Flinders University, Adelaide, SA 5042, Australia
3
Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia
*
Author to whom correspondence should be addressed.
Equal contribution.
Academic Editors: Rui Fausto and Scott Reed
Molecules 2021, 26(14), 4273; https://doi.org/10.3390/molecules26144273
Received: 17 June 2021 / Revised: 7 July 2021 / Accepted: 12 July 2021 / Published: 14 July 2021
Protein folding is important for protein homeostasis/proteostasis in the human body. We have established the ability to manipulate protein unfolding/refolding for β-lactoglobulin using the induced mechanical energy in the thin film microfluidic vortex fluidic device (VFD) with monitoring as such using an aggregation-induced emission luminogen (AIEgen), TPE-MI. When denaturant (guanidine hydrochloride) is present with β-lactoglobulin, the VFD accelerates the denaturation reaction in a controlled way. Conversely, rapid renaturation of the unfolded protein occurs in the VFD in the absence of the denaturant. The novel TPE-MI reacts with exposed cysteine thiol when the protein unfolds, as established with an increase in fluorescence intensity. TPE-MI provides an easy and accurate way to monitor the protein folding, with comparable results established using conventional circular dichroism. The controlled VFD-mediated protein folding coupled with in situ bioprobe AIEgen monitoring is a viable methodology for studying the denaturing of proteins. View Full-Text
Keywords: vortex fluidic device; protein folding/unfolding; aggregation induced emission vortex fluidic device; protein folding/unfolding; aggregation induced emission
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MDPI and ACS Style

Hu, Q.; Hu, H.; Zhang, X.; Fan, K.; Hong, Y.; Raston, C.L.; Tang, Y. In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe. Molecules 2021, 26, 4273. https://doi.org/10.3390/molecules26144273

AMA Style

Hu Q, Hu H, Zhang X, Fan K, Hong Y, Raston CL, Tang Y. In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe. Molecules. 2021; 26(14):4273. https://doi.org/10.3390/molecules26144273

Chicago/Turabian Style

Hu, Qi, Haozhen Hu, Xinyi Zhang, Kyle Fan, Yuning Hong, Colin L. Raston, and Youhong Tang. 2021. "In Situ Monitored Vortex Fluidic-Mediated Protein Refolding/Unfolding Using an Aggregation-Induced Emission Bioprobe" Molecules 26, no. 14: 4273. https://doi.org/10.3390/molecules26144273

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