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Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model

1
Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, Korea
2
Natural Product Informatics Research Center, Korea Institute of Science and Technology, Gangneung 25451, Korea
3
Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Maria Carla Marcotullio
Molecules 2019, 24(7), 1435; https://doi.org/10.3390/molecules24071435
Received: 18 March 2019 / Revised: 5 April 2019 / Accepted: 10 April 2019 / Published: 11 April 2019
(This article belongs to the Section Natural Products Chemistry)
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Abstract

Humulus japonicus is an annual plant belonging to the Cannabacea family, and it has been traditionally used to treat pulmonary tuberculosis, dysentery, chronic colitis, and hypertension. We investigated the active components against Parkinson’s disease from H. japonicus fraction (HJF) using high performance liquid chromatography (HPLC) coupled with quadruple-time-of-flight mass spectroscopy (qTOF-MS) and NMR. Fourteen compounds were isolated from HJF, including one new compound, using HPLC-qTOF-MS and NMR. The major compounds of HJF were luteolin-7-O-glucoside and apigenin-7-O-glucoside, and there was approximately 12.57- and 9.68-folds increase in the contents of these flavonoids compared to those of the 70% EtOH extract. Apigenin and luteolin exhibited the strongest inhibitory effects on monoamine oxidase (MAO) B enzyme activity. In animal studies, limb-use behavior was significantly reduced by unilateral 6-OHDA lesion and ipsilateral rotations. These results indicated that oral administration of 300 mg/kg HJF resulted in the improvement of motor asymmetry and motor impairment in unilateral 6-OHDA-lesioned mice. HJF, including active components leads to an improvement of motor behavior in a Parkinson’s disease mouse model. View Full-Text
Keywords: Humulus japonicus; HPLC-qTOF-MS; in vitro MAO B assay; in vivo 6-OHDA-lesioned PD animal test; Parkinson’s Disease Humulus japonicus; HPLC-qTOF-MS; in vitro MAO B assay; in vivo 6-OHDA-lesioned PD animal test; Parkinson’s Disease
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Lee, H.J.; Dhodary, B.; Lee, J.Y.; An, J.-P.; Ryu, Y.-K.; Kim, K.-S.; Lee, C.-H.; Oh, W.K. Dereplication of Components Coupled with HPLC-qTOF-MS in the Active Fraction of Humulus japonicus and It’s Protective Effects against Parkinson’s Disease Mouse Model. Molecules 2019, 24, 1435.

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