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Open AccessArticle

Coagulant Effects and Mechanism of Schefflera heptaphylla (L.) Frodin

by Xuqiang Liu 1,2,3,†, Jing Dong 1,†, Qiongxin Liang 1, Hui-min David Wang 4, Zhenhua Liu 1,*, Ruian Xu 2,3,* and Wenyi Kang 1,*
National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, China
Engineering Research center of Molecular Medicine, Ministry of Education, Huaqiao University, Xiamen 361021, China
School of Biomedical Sciences and School of Medicine, Huaqiao University, Xiamen 361021, China
Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung City 402, Taiwan
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Luisa Tesoriere and Alessandro Attanzio
Molecules 2019, 24(24), 4547;
Received: 13 November 2019 / Revised: 4 December 2019 / Accepted: 10 December 2019 / Published: 12 December 2019
(This article belongs to the Special Issue Cytotoxic Activity of Plant Extracts)
Schefflera heptaphylla (L.) Frodin, are commonly used in anti-inflammatory, analgesic, traumatic bleeding and hemostasisas. In this paper, the coagulation effect of the ethanol extract (Set), ethyl acetate phase (Sea) and n-butanol phase (Sbu) was evaluated by prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen content (FIB) assays in vitro. Then, Three main lupanine triterpenes (compounds AC) were isolated and identified from Sea and Sbu by a bioassay-guided method and their structure were identified as 3α-Hydroxy-lup-20(29)-ene-23, 28-dioic acid, betulinic acid 3-O-sulfate and 3α-Hydroxy-lup-20(29)-ene-23, 28-dioic acid 28-O-(α-l-rhamnopyranosyl(1→4)-O-β-d-glucopyranosyl(1→6))-β-d-glucopyranoside) by spectroscopic data analysis. Among of them, compound B was confirmed to have significant coagulant effect in vitro. Furthermore, the pro-coagulation mechanism of S. heptaphylla extracts and compound B were investigated by measuring whole blood viscosity (WBV), plasma viscosity (PV), erythrocyte sedimentetion rate (ESR), pack cell volume (PCV), APTT, PT, TT, and FIB in vivo. Meanwhile, the levels of thromboxane B2 (TXB2), 6-keto prostaglandin F (6-keto-PGF), endothelial nitric oxide synthase (eNOS) and (endothelin-1) ET-1 were detected. The bleeding time (BT) was tested by tail bleeding method, which proved the traumatic bleeding and hemostasis activities of S. heptaphylla. The pharmacology experiments showed that the Set, Sea, Sbu and compound B has significant pro-coagulation effect. In addition, compound B might be the main constituent of pro-coagulation in S. heptaphylla These results could support the fact that S. heptaphylla could be used traditionally to cure traumatic bleeding, and the pro-coagulation effects were associated with the regulation of vascular endothelium active substance and hemorheology parameters. View Full-Text
Keywords: Schefflera heptaphylla; Triterpenoid; hemostasis; pro-coagulation; mechanism Schefflera heptaphylla; Triterpenoid; hemostasis; pro-coagulation; mechanism
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MDPI and ACS Style

Liu, X.; Dong, J.; Liang, Q.; Wang, H.-M.D.; Liu, Z.; Xu, R.; Kang, W. Coagulant Effects and Mechanism of Schefflera heptaphylla (L.) Frodin. Molecules 2019, 24, 4547.

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