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Molecules 2018, 23(6), 1477; https://doi.org/10.3390/molecules23061477

Desmoglein1 Deficiency Is a Potential Cause of Cutaneous Eruptions Induced by Shuanghuanglian Injection

1
Chinese Materia Medica College, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
2
Tianjin Academy of Traditional Chinese Medicine, Tianjin 300120, China
3
Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Yidan Zhang and Xiujun Zhang contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 31 May 2018 / Accepted: 13 June 2018 / Published: 19 June 2018
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Abstract

Cutaneous eruption is a common drug-adverse reaction, characterised by keratinocytes inflammation and apoptosis. Shuanghuanglian injeciton (SHLI) is a typical Chinese medicine injection, which is used to treat influenza. It has been reported that SHLI has the potential to induce cutaneous adverse eruptions. However, the mechanisms remain unclear. Since desmoglein 1 (DSG1) shows a crucial role in maintaining skin barrier function and cell susceptibility, we assume that DSG1 plays a critical role in the cutaneous eruptions induced by SHLI. In our study, retinoic acid (RA) was selected to downregulate the DSG1 expression, and lipopolysaccharide (LPS) was first used to identify the susceptibility of the DSG1-deficiency Hacat cells. Then, SHLI was administrated to normal or DSG1-deficient Hacat cells and mice. The inflammatory factors and apoptosis rate were evaluated by RT-PCR and flow cytometry. The skin pathological morphology was observed by hematoxylin and eosin (HE) staining. Our results show that treated only with SHLI could not cause IL-4 and TNF-α mRNA increases in normal Hacat cells. However, in the DSG1-deficient Hacat cells or mice, SHLI induced an extreme increase of IL-4 and TNF-α mRNA levels, as well as in the apoptosis rate. The skin tissue showed a local inflammatory cell infiltration when treated with SHIL in the DSG1-deficient mice. Thus, we concluded that DSG1 deficiency was a potential causation of SHLI induced eruptions. These results indicated that keratinocytes with DSG1 deficiency were likely to induce the cutaneous eruptions when stimulated with other medicines. View Full-Text
Keywords: desmoglein1; cutaneous eruptions; keratinocyte; inflammation; apoptosis desmoglein1; cutaneous eruptions; keratinocyte; inflammation; apoptosis
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Zhang, Y.; Zhang, X.; Fan, S.; Song, L.; Yang, Z.; Zhuang, P.; Zhang, Y. Desmoglein1 Deficiency Is a Potential Cause of Cutaneous Eruptions Induced by Shuanghuanglian Injection. Molecules 2018, 23, 1477.

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