Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.) Poir. and Isolation of 5-O-β-d-Glucopyranosyljusticidin B and Six Other Lignans
Abstract
:1. Introduction
2. Results and Discussion
2.1. Pharmacological Assays
2.1.1. Carrageenan-Induced Rat Paw Edema
2.1.2. Dextran-Induced Rat Paw Edema
2.1.3. Formalin Test
2.1.4. Acute Oral Toxicity
2.2. General Chemistry
2.3. Isolation of Compounds
3. Materials and Methods
3.1. Pharmacological Assays
3.1.1. Drugs and Chemical Compounds
3.1.2. Animals
3.1.3. Carrageenan-Induced Rat Paw Edema
3.1.4. Dextran-Induced Rat Paw Edema
3.1.5. Formalin Test
3.1.6. Acute Oral Toxicity
3.1.7. Statistical Analysis
3.2. Collection, Identification and Preparation of Crude Extract
3.3. General Chemistry
3.4. Isolation of Compounds
3.4.1. HPLC Profile
3.4.2. Compound 3
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Moiety | Position | 3 | |||
---|---|---|---|---|---|
δC | δH (J in Hz) | HMBC (H to C) | COSY (H to H) | ||
Aglycone moiety | 1 | 140.4 a | |||
2 | 120.8 | ||||
3 | 140.5 a | ||||
4 | 116.9 | 8.45 s | 2, 5, 10 | 12 | |
5 | 145.4 | ||||
6 | 144.4 | ||||
7 | 154.6 | ||||
8 | 104.0 | 6.98 s | 1, 7 | OCH3-7 | |
9 | 129.8 b | ||||
10 | 131.4 b | ||||
11 | 172.3 | ||||
12 | 70.1 | 5.43 s | 3, 4, 11 | 4 | |
1′ | 130.3 | ||||
2′ | 111.5 | 6.78 d (1.5) | 3′, 4′, 6′ | 6′ | |
3′ | 149.0 | ||||
4′ | 149.0 | ||||
5′ | 109.0 | 6.95 d (7.8) | 1′, 3′, 4′ | ||
6′ | 124.6 | 6.75 dd (1.5, 7.8) | 2′ | 2′ | |
7′ | 102.6 | 6.03 d (1.2) 6.04 d (1.2) | 3′, 4′ | ||
OCH3-6 | 62.0 | 3.97 s | 6 | ||
OCH3-7 | 56.1 | 3.74 s | 7 | 8 | |
Sugar moiety | 1″ | 105.7 | 5.16 d (7.8) | 5 | |
2″ | 75.7 | 3.65 m | |||
3″ | 77.9 | 3.49 m | |||
4″ | 71.3 | 3.46 m | |||
5″ | 78.3 | 3.20 m | |||
6″ | 62.2 | 3.69 m |
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Borges, L.D.C.; Negrão-Neto, R.; Pamplona, S.; Fernandes, L.; Barros, M.; Fontes-Júnior, E.; Maia, C.; E Silva, C.Y.Y.; Silva, M.N.d. Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.) Poir. and Isolation of 5-O-β-d-Glucopyranosyljusticidin B and Six Other Lignans. Molecules 2018, 23, 941. https://doi.org/10.3390/molecules23040941
Borges LDC, Negrão-Neto R, Pamplona S, Fernandes L, Barros M, Fontes-Júnior E, Maia C, E Silva CYY, Silva MNd. Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.) Poir. and Isolation of 5-O-β-d-Glucopyranosyljusticidin B and Six Other Lignans. Molecules. 2018; 23(4):941. https://doi.org/10.3390/molecules23040941
Chicago/Turabian StyleBorges, Luziane Da C., Raimundo Negrão-Neto, Sônia Pamplona, Luanna Fernandes, Mayra Barros, Enéas Fontes-Júnior, Cristiane Maia, Consuelo Y. Yoshioka E Silva, and Milton Nascimento da Silva. 2018. "Anti-Inflammatory and Antinociceptive Studies of Hydroalcoholic Extract from the Leaves of Phyllanthus brasiliensis (Aubl.) Poir. and Isolation of 5-O-β-d-Glucopyranosyljusticidin B and Six Other Lignans" Molecules 23, no. 4: 941. https://doi.org/10.3390/molecules23040941