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Molecules 2018, 23(4), 758; https://doi.org/10.3390/molecules23040758

UHPLC-MS/MS Quantification Combined with Chemometrics for Comparative Analysis of Different Batches of Raw, Wine-Processed, and Salt-Processed Radix Achyranthis Bidentatae

1
Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China
2
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 528458, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 2 February 2018 / Revised: 5 March 2018 / Accepted: 22 March 2018 / Published: 26 March 2018
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Abstract

An accurate and reliable method using ultra-high performance liquid chromatography combined with triple quadrupole tandem mass spectrometry (UHPLC–MS/MS) was established for simultaneous quantification of five major bioactive analytes in raw, wine-processed, and salt-processed Radix Achyranthis bidentatae (RAB). The results showed that this method exhibited desirable sensitivity, precision, stability, and repeatability. The overall intra-day and inter-day variations (RSD) were in the range of 1.57–2.46 and 1.51–3.00%, respectively. The overall recoveries were 98.58–101.48% with a relative standard deviation (RSD) of 0.01–1.86%. In addition, the developed approach was applied to 21 batches of raw, wine-processed, and salt-processed samples of RAB. Hierarchical clustering analysis (HCA), principal component analysis (PCA), heat map, and boxplot analysis were performed to evaluate the quality of raw, wine-processed, and salt-processed RAB collected from different regions. The chemometrics combined with the quantitative analysis based on UHPLC–MS/MS results indicated that the content of five analytes increased significantly in processed RAB compared to raw RAB. View Full-Text
Keywords: Radix Achyranthis bidentatae; UHPLC–MS/MS; processing; quality assessment; chemometrics Radix Achyranthis bidentatae; UHPLC–MS/MS; processing; quality assessment; chemometrics
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Yang, L.; Jiang, H.; Yan, M.; Xing, X.; Guo, X.; Yang, B.; Wang, Q.; Kuang, H. UHPLC-MS/MS Quantification Combined with Chemometrics for Comparative Analysis of Different Batches of Raw, Wine-Processed, and Salt-Processed Radix Achyranthis Bidentatae. Molecules 2018, 23, 758.

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