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Molecules 2018, 23(4), 727; https://doi.org/10.3390/molecules23040727

Heterogeneity of Circulating Tumor Cells in Neoadjuvant Chemotherapy of Breast Cancer

1
Cancer Research Institute, Tomsk National Research Medical Center, Tomsk 634050, Russia
2
Siberian State Medical University, Tomsk 634050, Russia
3
Laboratory for Translational Cellular and Molecular Biomedicine, Tomsk State University, Tomsk 634050, Russia
*
Author to whom correspondence should be addressed.
Academic Editors: Samuel Achilefu and Derek J. McPhee
Received: 16 January 2018 / Revised: 14 March 2018 / Accepted: 18 March 2018 / Published: 22 March 2018
(This article belongs to the Special Issue Molecular Imaging and Treatment Monitoring of Cancer)
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Abstract

The biological properties of circulating tumor cells (CTCs), and their dynamics during neoadjuvant chemotherapy are important, both for disease progression prediction and therapeutic target determination, with the aim of preventing disease progression. The aim of our study was to estimate of different CTC subsets in breast cancer during the NACT (neoadjuvant chemotherapy). The prospective study includes 27 patients with invasive breast cancer, T2-4N0-3M0, aged 32 to 60 years. Venous heparinized blood samples, taken before and after biopsy, after each courses of chemotherapy (on days 3–7), and before surgical intervention, served as the material for this study. Different subsets of circulating tumor cells were determined on the basis of the expression of EpCAM, CD45, CD44, CD24, and N-Cadherin using flow cytometry. As the result of this study, it has been observed that significant changes in the quantity of the different subsets of circulating tumor cells in patients’ blood were observed after carrying out the 3rd course of NACT. NACT causes significant changes in the quantity of six CTC subsets, with various combinations of stemness and epithelial-mesenchymal transition (EMT) properties. View Full-Text
Keywords: heterogeneity of circulating tumor cells; EMT; EpCAM-negative circulating tumor cells; NACT; breast cancer heterogeneity of circulating tumor cells; EMT; EpCAM-negative circulating tumor cells; NACT; breast cancer
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Kaigorodova, E.V.; Savelieva, O.E.; Tashireva, L.A.; Tarabanovskaya, N.A.; Simolina, E.I.; Denisov, E.V.; Slonimskaya, E.M.; Choynzonov, E.L.; Perelmuter, V.M. Heterogeneity of Circulating Tumor Cells in Neoadjuvant Chemotherapy of Breast Cancer. Molecules 2018, 23, 727.

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