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Molecules 2018, 23(11), 2999; https://doi.org/10.3390/molecules23112999

Differential Effects of the Flavonolignans Silybin, Silychristin and 2,3-Dehydrosilybin on Mesocestoides vogae Larvae (Cestoda) under Hypoxic and Aerobic In Vitro Conditions

1
Department of Experimental Pharmacology, Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, SK 040 01 Košice, Slovakia
2
Laboratory of Molecular Structure Characterization, Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ 142 20 Prague, Czech Republic
3
Department of Pharmacognosy, Faculty of Pharmacy, Charles University, Heyrovského 1203, CZ 501 65 Hradec Králové, Czech Republic
4
Laboratory of Biotransformation, Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ 142 20 Prague, Czech Republic
*
Authors to whom correspondence should be addressed.
Received: 17 October 2018 / Revised: 8 November 2018 / Accepted: 15 November 2018 / Published: 16 November 2018
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Abstract

Mesocestoides vogae larvae represent a suitable model for evaluating the larvicidal potential of various compounds. In this study we investigated the in vitro effects of three natural flavonolignans—silybin (SB), 2,3-dehydrosilybin (DHSB) and silychristin (SCH)—on M. vogae larvae at concentrations of 5 and 50 μM under aerobic and hypoxic conditions for 72 h. With both kinds of treatment, the viability and motility of larvae remained unchanged, metabolic activity, neutral red uptake and concentrations of neutral lipids were reduced, in contrast with a significantly elevated glucose content. Incubation conditions modified the effects of individual FLs depending on their concentration. Under both sets of conditions, SB and SCH suppressed metabolic activity, the concentration of glucose, lipids and partially motility more at 50 μM, but neutral red uptake was elevated. DHSB exerted larvicidal activity and affected motility and neutral lipid concentrations differently depending on the cultivation conditions, whereas it decreased glucose concentration. DHSB at the 50 μM concentration caused irreversible morphological alterations along with damage to the microvillus surface of larvae, which was accompanied by unregulated neutral red uptake. In conclusion, SB and SCH suppressed mitochondrial functions and energy stores, inducing a physiological misbalance, whereas DHSB exhibited a direct larvicidal effect due to damage to the tegument and complete disruption of larval physiology and metabolism. View Full-Text
Keywords: silybin; 2,3-dehydrosilybin; silychristin; Mesocestoides vogae larvae; aerobic and hypoxic cultivation silybin; 2,3-dehydrosilybin; silychristin; Mesocestoides vogae larvae; aerobic and hypoxic cultivation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Hrčková, G.; Kubašková, T.M.; Benada, O.; Kofroňová, O.; Tumová, L.; Biedermann, D. Differential Effects of the Flavonolignans Silybin, Silychristin and 2,3-Dehydrosilybin on Mesocestoides vogae Larvae (Cestoda) under Hypoxic and Aerobic In Vitro Conditions. Molecules 2018, 23, 2999.

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