Next Article in Journal
Pratol, an O-Methylated Flavone, Induces Melanogenesis in B16F10 Melanoma Cells via p-p38 and p-JNK Upregulation
Previous Article in Journal
Phosphorus-Sulfur Heterocycles Incorporating an O-P(S)-O or O-P(S)-S-S-P(S)-O Scaffold: One-Pot Synthesis and Crystal Structure Study
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(10), 1659;

Effect of High-Pressure Treatment on Catalytic and Physicochemical Properties of Pepsin

School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China
Author to whom correspondence should be addressed.
Received: 19 September 2017 / Revised: 29 September 2017 / Accepted: 2 October 2017 / Published: 11 October 2017
Full-Text   |   PDF [2075 KB, uploaded 11 October 2017]   |  


For a long time, high-pressure treatment has been used to destroy the compact structures of natural proteins in order to promote subsequent enzymatic hydrolysis. However, there are few reports evaluating the feasibility of directly improving the catalytic capability of proteases by using high-pressure treatments. In this study, the effects of high-pressure treatment on the catalytic capacity and structure of pepsin were investigated, and the relationship between its catalytic properties and changes in its physicochemical properties was explored. It was found that high-pressure treatment could lead to changes of the sulfhydryl group/disulfide bond content, hydrophobicity, hydrodynamic radius, intrinsic viscosity, and subunit composition of pepsin, and the conformational change of pepsin resulted in improvement to its enzymatic activity and hydrolysis efficiency, which had an obvious relationship with the high-pressure treatment conditions. View Full-Text
Keywords: high-pressure treatment; pepsin; enzymatic hydrolysis; physicochemical properties high-pressure treatment; pepsin; enzymatic hydrolysis; physicochemical properties

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Wang, J.; Bai, T.; Ma, Y.; Ma, H. Effect of High-Pressure Treatment on Catalytic and Physicochemical Properties of Pepsin. Molecules 2017, 22, 1659.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top