5-Hydroxycyclopenicillone Inhibits β-Amyloid Oligomerization and Produces Anti-β-Amyloid Neuroprotective Effects In Vitro
Abstract
:1. Introduction
2. Results
2.1. 5-Hydroxycyclopenicillone Inhibits Aβ1-42 Oligomer Formation
2.2. 5-Hydroxycyclopenicillone Likely Binds to Aβ1-42 Peptides via Hydrophobic Interactions
2.3. 5-Hydroxycyclopenicillone Decreased the Neurotoxicity of the Aβ1-42 Oligomer in SH-SY5Y Cells
2.4. 5-Hydroxycyclopenicillone Prevents Synaptic Toxicity of Aβ1-42 Oligomer in Primary Hippocampal Neurons
3. Discussion
4. Materials and Methods
4.1. Preparation of 5-Hydroxycyclopenicillone
4.2. Preparation of the Aβ1-42 Oligomer
4.3. Dot Blotting Analysis
4.4. TEM Analysis
4.5. Simulation System
4.6. Molecular Dynamics Simulation
4.7. Molecular Dynamics Simulation Analysis
4.8. Culture of SH-SY5Y Cells
4.9. Cell Viability Measurement
4.10. FDA/PI Double Staining
4.11. Primary Hippocampal Neuronal Cultures
4.12. Immunocyto Chemisty
4.13. Confocal Imaging and Analysis
4.14. Data Analysis and Statistics
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
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Zhao, J.; Liu, F.; Huang, C.; Shentu, J.; Wang, M.; Sun, C.; Chen, L.; Yan, S.; Fang, F.; Wang, Y.; et al. 5-Hydroxycyclopenicillone Inhibits β-Amyloid Oligomerization and Produces Anti-β-Amyloid Neuroprotective Effects In Vitro. Molecules 2017, 22, 1651. https://doi.org/10.3390/molecules22101651
Zhao J, Liu F, Huang C, Shentu J, Wang M, Sun C, Chen L, Yan S, Fang F, Wang Y, et al. 5-Hydroxycyclopenicillone Inhibits β-Amyloid Oligomerization and Produces Anti-β-Amyloid Neuroprotective Effects In Vitro. Molecules. 2017; 22(10):1651. https://doi.org/10.3390/molecules22101651
Chicago/Turabian StyleZhao, Jiaying, Fufeng Liu, Chunhui Huang, Jieyi Shentu, Minjun Wang, Chenkai Sun, Liping Chen, Sicheng Yan, Fang Fang, Yuanyuan Wang, and et al. 2017. "5-Hydroxycyclopenicillone Inhibits β-Amyloid Oligomerization and Produces Anti-β-Amyloid Neuroprotective Effects In Vitro" Molecules 22, no. 10: 1651. https://doi.org/10.3390/molecules22101651