Next Article in Journal
Design, Synthesis, and Biological Evaluation of a New Series of Biphenyl/Bibenzyl Derivatives Functioning as Dual Inhibitors of Acetylcholinesterase and Butyrylcholinesterase
Previous Article in Journal
Toxicity of Plant Secondary Metabolites Modulating Detoxification Genes Expression for Natural Red Palm Weevil Pesticide Development
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 22
Issue 1
10.3390/molecules22010170
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Synthesis and Anticancer Activity of New 1-Thia-4-azaspiro[4.5]decane, Their Derived Thiazolopyrimidine and 1,3,4-Thiadiazole Thioglycosides

by
Eman M. Flefel
1,2,
Wael A. El-Sayed
2,*,
Ashraf M. Mohamed
3,4,
Walaa I. El-Sofany
2,* and
Hanem M. Awad
5
1
Department of Chemistry, College of Science, Taibah University, Al-Madinah Al-Monawarah 1343, Saudi Arabia
2
Photochemistry Department, National Research Centre, Dokki 12622, Giza, Egypt
3
Applied Organic Chemistry Department, National Research Centre, Dokki 12622, Giza, Egypt
4
Chemistry Department, College of Science, Aljouf University, Sakaka, Al-Jouf 72341, Saudi Arabia
5
Department of Tanning Materials and Leather Technology, National Research Centre, Dokki 12622, Giza, Egypt
*
Authors to whom correspondence should be addressed.
Molecules 2017, 22(1), 170; https://doi.org/10.3390/molecules22010170
Submission received: 24 December 2016 / Revised: 10 January 2017 / Accepted: 16 January 2017 / Published: 20 January 2017
Browse Figures
Versions Notes

Abstract

:
New 1-thia-azaspiro[4.5]decane derivatives, their derived thiazolopyrimidine and 1,3,4-thiadiazole compounds were synthesized. The thioglycoside derivatives of the synthesized (1,3,4-thiadiazolyl)thiaazaspiro[4.5]decane and thiazolopyrimidinethione compounds were synthesized by glycosylation reactions using acetylated glycosyl bromides. The anticancer activity of synthesized compounds was studied against the cell culture of HepG-2 (human liver hepatocellular carcinoma), PC-3 (human prostate adenocarcinoma) and HCT116 (human colorectal carcinoma) cell lines and a number of compounds showed moderate to high inhibition activities.

1. Introduction

Chemotherapy is considered as an important option [1] among applied strategies for cancer treatment and the major objective of many approved chemotherapeutic agents [2], is the induction of apoptosis of cancer cells. Research for scouting novel selective anticancer agents, with minimal side effects, is a demanding requirement because of toxicity to normal cells, decreased drug activity and development of drug resistance, which are responsible for insufficiency in cancer treatment.
It has been found that the thiazolidine ring system is mainly integrated in important compounds with interesting applications in medicinal and pharmaceutical chemistry. A variety of established drugs and drug candidates such as Pioglitazone, Epalrestat, Letosteine, and Tidiacichave, have in their skeleton, a thiazolidine nucleus. Such a nucleus is described as a wonder moiety because of the wide spectrum of biological activities associated with its derivatives. The N–C–S linkage had been found to be responsible, in active compounds, for exhibiting antimicrobial [3,4,5] and anti-HIV [6,7] activities. The thiazolidine nucleus, notably 1,4-thiazolidinedione (TZD), has been widely employed as a unique scaffold in developing new potent anticancer agents showing cytotoxicity against different human cancer cells [8,9,10] and recently was intensified as a novel onset in cancer chemotherapy [11,12]. Efatutazone, netoglitazone, rosiglitazone and troglitazone, having the thiazolidine system in their basic skeleton, are being studied regarding the mechanism underlying their anticancer activity [11].
Although spiro-compounds allow conformational qualifications and original orientation of the diversity of elements, spirothiazolidines have been poorly investigated. This scaffold is believed to be a precious isostere for known active spiroimidazolidines, spirohydantoines or spirobenzofuranes, with implementations in G protein–coupled receptors (GPCRs) and peptidomimetics. It is incorporated in Spiclomazine, an antipsychotic drug. Simple spirothiazolidines have been revealed for their inhibition activity of metalloprotease which allows their application in the control of malaria infection [13]. Spirothiazolidine derivatives were also found to be of good activity as anticancer and antioxidant [14] agents. Thiazolopyrimidine is an interesting bicyclic system as its derivatives revealed their activity as analgesic and antiparkinsonian agents [15], Transient Receptor Potential Vanilloid–receptor 1 (TRPV1) modulators [16], anticancer agents [17,18,19,20], antioxidants [21], pesticides [22], phosphate inhibitors [23,24], acetylcholinesterase inhibitors [25] and antimicrobial substances [26,27,28]. Structures A–D (Figure 1) with spiro-thiazolidine, thiazolopyrimidine and 1,3,4-thiadiazole ring systems have been reported with their anticancer activity [14,29,30].
Thioglycosides and their analogs incorporating modified glycosyl and/or aglycon parts have prompted extended research as biological inhibitors [31,32,33]. A number of thioglycosides have been reported with their potential biological activities in addition to their use as glycosyl donors [32,33,34,35]. The above findings and our continued research program targeting synthesis of new glycosyl heterocycles [33,35,36,37,38] and their acyclic analogs with anticancer activity have prompted us to synthesize new thia-azaspiro[4.5]decane, their derived thiazolopyrimidine and 1,3,4-thiadiazole thioglycosides with anticancer evaluation studies.

2. Results and Discussion

2.1. Chemistry

The thia-4-azaspiro[4.5]decan compounds 1–3 were prepared via a one-pot three-component reaction involving condensation of ketones namely, 4-methylcyclohexanone and cyclohexanone; aromatic amines namely, 4-bromoaniline or 4-fluoroaniline; and mercaptoacetic acid in dry benzene to afford the corresponding substituted aryl-1-thia-4-azaspiro[4.5]decan-3-one compounds 1–3 [14]. The Infra-Red spectra of the resulting spiro-thiazolidine derivatives 1 and 3 showed characteristic peaks at 1682 and 1677 cm−1 corresponding to the thiazolidinone carbonyl function. The 1H-NMR spectra of compounds 1 and 3 displayed a singlet signal at 0.88 and 0.86 ppm to methyl protons while the signal attributed to methylene protons of the thiazolidinone ring appeared at 3.39 and 3.37 ppm. Their 13C-NMR spectrum showed characteristic peaks corresponding to the C=O group and the signals assigned to the spiro-C atom. Condensation of the fluoro-substituted derivative 2 with 2-methylfurfural in ethanol under reflux in the presence of a base led to the arylidene derivative 4 formation in 74% yield. The IR spectrum of the afforded furyl-arylidene 4 showed the band at 1672 cm−1 corresponding to the C=O group. Its 1H-NMR spectrum revealed the disappearance of the signals of the thiazolidine-CH2 protons and existence of signals assigned to the methine and aryl hydrogens. The 13C-NMR spectrum showed signals at δ 73.35 for the spiro-carbon, 116.4–160.1 corresponding to the sp2 carbons and 167.9 for the carbonyl group. When compound 4 was fused with thiourea in the presence of a catalytic amount of piperidine at 180 °C, it afforded the thiazolo[4,5-d]pyrimidine]-5′(6′H)-thione derivative 5 for which the obtained spectral characteristics confirmed its assigned structure. The 1H-NMR spectrum showed the signal at 13.25 for NH tautomerized with C=S in addition to cyclohexyl and aryl protons.
The reaction of the thiazolopyrimidinethione derivative 5 with 2,3,4,6-tetra-O-acetyl-α-d-gluco- or 2,3,4,-tri-O-acetyl-α-d-xylopyranosyl bromide 6a,b afforded the corresponding glycosylthio-derivatives of the thiazolopyrimidine nucleus 7 and 9, respectively. The 1H-NMR spectra of the resulting thioglycosides showed the signal corresponding to acetyl methyl protons in addition to the other protons of the sugar chain. The formation of these thioglycosides and linkage of the sugar part at a sulfanyl center was confirmed by the NMR data. The relatively low chemical shift of the anomeric proton (H-1) in the 1H-NMR and the absence of the C=S signal in the 13C-NMR spectra indicates such a mode of attachment. In N-glycosides, the anomeric proton is known to be found at relatively higher chemical shift values (5.95–6.15) [39,40] as a result of the deshielding influence of the thione function. The coupling constant of the anomeric proton also revealed the β-thioglycosidic linkage of the sugar moiety in the produced thioglycosides. Deacetylation of the latter glycosides 7 and 8 with methanolic ammonia solution led to the formation of the corresponding free hydroxyl derivatives 9 and 10, respectively (Scheme 1). Their IR spectra indicated the disappearance of acetyl carbonyl groups and the appearance of characteristic peaks in the hydroxyl frequency area attributed to sugar hydroxyls and the 1H-NMR spectra are agreeing with their assigned structure.
Nucleophilic addition between primary amine and carbonyl aldehyde/ketone to prepare Schiff bases is also common for amino-substituted thiadiazoles [41,42]. The pair of electrons of the nitrogen in the NH2 allows the nucleophilic substitution process to be easily performed [43]. Condensation of compounds 2 and 3 with 2-amino-1,3,4-thiadiazole-5-thiol (11) in ethanol gave the corresponding arylidine-1,3,4-thiadiazole-2-thione derivatives 12 and 13, respectively. The absence of the carbonyl band in the IR spectra and the presence of the NH group in their 1H-NMR spectra confirmed the assigned structures. Glycosylation of the afforded latter 1,3,4-thiadiazole derivatives via reaction with O-acetyl-α-d-gluco- or pyranosyl bromide compounds 6a,b, led to the formation of the corresponding 1,3,4-thiadiazole thioglycoside derivatives 14–17 in 65%–75% yields. The IR spectral data showed the bands corresponding to the glycosyl acetyl carbonyl groups in the C=O frequency zone. The chemical shift values and the coupling constant values in their obtained NMR spectral data have revealed the β-glycosidic linkage nature at the sulfur atom in the form of 1,3,4-thiadiazole thioglycosides. The deportation (deacetylation) reaction was carried out for two of the latter synthesized thioglycosides; one has the glucopyranosyl moiety (six carbon sugar part) and the other incorporates a xylopyranosyl unit (five carbon glycosyl). Thus, the free hydroxyl thioglycosides of the (thiadiazolyl)thia-4-azaspiro[4.5]decan incorporating the substituted cyclohexyl ring 18 and 19 were obtained by deacetylation reactions of the their acetylated precursors with methanolic ammonia solution (Scheme 2). The absence of the acetyl carbonyl bands in the IR spectra of the latter unprotected thioglycosides, in addition to the appearance of the hydroxyl absorption bands confirmed the assigned structures. Furthermore, their 1H-NMR spectra showed signals corresponding to the sugar hydroxyls and chain protons, in addition to the disappearance of the acetyl-methyl signals.

2.2. Anti-Tumor Activity

The synthesized compounds were examined in vitro for their anti-tumor activities against human liver hepatocellular carcinoma (HepG-2), human prostate adenocarcinoma (PC-3) and human colorectal carcinoma (HCT-116) cell lines using a MTT assay. The percentage of the intact cells was measured and compared to the control (Figure 2). The IC50 values of tested compounds are shown in Table 1.
The activities of these compounds against the three carcinoma cells were compared with that of Doxorubicin®.
The obtained results showed that all compounds showed dose-dependent anticancer activities against the three cancer cells. Compounds which did not show anticancer activity against the previously mentioned cell lines at concentration 100 ppm or more have not been investigated for IC50 values. The results outlined in Figure 2 and Table 1 indicated that five compounds (7, 9, 14, 18 and 19) showed good anticancer activities against HCT-116 carcinoma cells with IC50 ranging from 92.2 to 120.1 nM. The rest of the compounds showed moderate activities against HCT-116 cells.
The activity results against PC-3 cancer cells revealed that compounds 14 and 18 showed good anticancer activities. In addition, five compounds (1, 3, 7, 9 and 13) showed moderate inhibition activities against this type of cancer cells and the rest of the compounds showed weak activities. Furthermore, four compounds (7, 14, 17 and 9) showed moderate activities and the rest of the compounds showed weak anticancer activities against HepG-2 liver cancer.
Depending on the above results and on their correlation with the structures of highly active compounds, the obtained inhibition activity results of compounds 7, 9, 14, 17–19 revealed the importance of attachment of glycosyl moieties to the thiazolopyrimidine or (1,3,4-thiadiazolyl)thiazolidinone ring systems. It is clear that the activity was raised in the latter compounds in which the polyhydroxy or poly-acetylated cyclic sugar unit was linked via a thioglycosidic linkage to the 1,3,4-thiadiazole or thiazolopyrimidine ring systems compared to the glycosyl free heterocycles 5, 12 and 13. This conclusion was also supported by the obtained results for compound 4, with little activity against the previously mentioned cells, in which the thiazolidine ring was substituted with methylfurane substituent. Furthermore, the attachment of glucopyranosyl moiety to the thiazolopyrimidine or 1,3,4-thiadiazol ring system (compounds 7, 9, 14 and 18) led to an increase in inhibition activity more than the presence of xylopyranosyl moiety.
On the other hand, it can also be concluded that the attachment of the alkyl group at position-4 in the cyclohexyl ring in the tested spiro-compounds resulted in high activity against HCT-116 and PC-3 cancer cells. This is clear as the activity was decreased in compounds 2 and 12 in which the cyclohexyl group is free of substitution with methyl substituent.

3. Materials and Methods

3.1. Chemistry

All melting points are uncorrected and were determined on a Stuart electric melting point apparatus. The IR spectra were recorded on a Thermo Nicolet (Thermo Scientific, Madison, WI, USA) using KBr disks. The NMR spectra (500/125 MHz) were determined by using a Bruker NMR spectrometer (Rheinstetten, Munchen, Germany). A chemical shift was expressed in δ (ppm) downfield from tetramethylsilane (TMS) as an internal standard. Mass spectra were recorded at 70 eV EI Ms-QP 1000 EX (Shimadzu, Kyoto, Japan). The microanalysis was within ±0.4% of the theoretical values and was carried out at the Microanalytical Centre, National Research Centre, Cairo, Egypt. Homogeneity of all compounds synthesized was checked by thin layer chromatography (TLC) which was performed on Merck 60 (Munchen, Germany) ready-to-use silica gel plates to monitor the reactions and test the purity of the new synthesized compounds. The chemical names given for the prepared compounds are according to the International Union of Pure and Applied Chemistry (IUPAC) system.

3.1.1. 8-Substituted-4-aryl-1-thia-4-azaspiro[4.5]decan-3-one (1–3)

A mixture of cyclohexanone or methylcyclohexanone (0.01 mol), aromatic amine, namely, 4-bromoaniline or 4-flouroaniline (0.01 mol), and thioglycolic acid (0.01 mol, 0.92 mL) in dry benzene (50 mL) was refluxed for 10 h. The solution was concentrated; the formed solid was filtered off, dried, and crystallized to give compounds 1–3.
4-(4-Bromophenyl)-8-methyl-1-thia-4-azaspiro[4.5]decan-3-one (1). White powder, Yield: 77%; m.p. 130–131 °C; IR (KBr, υ, cm−1): 3055 (C-H aromatic), 2925 (C-H aliphatic), 1682 (C=O); 1H-NMR (DMSO-d6): δ (ppm) 0.88 (d, J = 6.2 Hz, 3H, CH3), 1.24–1.77 (m, 9H, cyclohexyl-H), 3.39 (s, 2H, CH2), 7.20 (d, J = 7.6 Hz, 2H, Ar-H), 7.32 (d, J = 7.6 Hz, 2H, Ar-H); 13C-NMR: 23.5–39.4 (5CH2), 43.8 (CH2), 74.2 (spiro-C), 115.4–154.1 (Ar-C), 172.2 (C=O); MS, m/z (%): 339 (M+ + 28), 341 (M+ + 2, 26). Analysis calc. for C15H18BrNOS (340.28): C, 52.95; H, 5.33; N, 4.12. Found: C, 52.74; H, 5.19; N, 4.19.
4-(4-Fluorophenyl)-1-thia-4-azaspiro[4.5]decan-3-one (2) [14]. This compound was prepared as reported earlier as pale yellow needle crystals; m.p. 142–143 °C (Reported m.p. 143–145 °C).
4-(4-Fluorophenyl)-8-methyl-1-thia-4-azaspiro[4.5]decan-3-one (3). Pale yellow powder, Yield: 72%; m.p. 125–126 °C; IR (KBr, υ, cm−1): 3058 (C-H aromatic), 2930 (C-H aliphatic), 1677 (C=O); 1H-NMR (DMSO-d6): δ (ppm) 0.86 (d, J = 6.4 Hz, 3H, CH3), 1.24–1.79 (m, 9H,5CH2), 3.37 (s, 2H, CH2), 7.22 (d, J = 7.8 Hz, 2H, Ar-H), 7.34 (d, J = 7.8 Hz, 2H, Ar-H); 13C-NMR: 23.1 (CH3), 24.0–39.5 (cyclohexyl-CH2 and -CH), 43.7 (CH2), 74.1 (spiro-C), 115.4–161.1 (Ar-C), 172.3 (C=O); MS, m/z (%): 279.3 (M+, 35). Analysis calc. for C15H18FNOS (279.37): C, 64.49; H, 6.49; N, 5.01. Found: C, 64.21; H, 6.31; N, 5.22.

3.1.2. 4-(4-Fluorophenyl)-2-((5-methylfuran-2-yl)methylene)-1-thia-4-azaspiro[4.5]decan-3-one (4)

A mixture of compound 2 (0.01 mol, 2.65 g) and 5-methylfurfural (0.01 mol, 1.1 g) in ethanolic sodium hydroxide solution (10%, 25 mL) was heated under reflux for 6 h. The reaction mixture was poured onto ice-cold water (50 mL) and neutralized with dilute HCl; then the formed solid was filtered off and crystallized from ethanol to give the arylidine derivative 4.
Brownish-red powder, Yield: 74%; m.p. 122–123 °C; IR (KBr, υ, cm−1): 3045 (C-H aromatic), 2940 (C-H aliphatic), 1672 (C=O); 1H-NMR (DMSO-d6): δ (ppm) 1.42–1.96 (m, 10H, 5CH2), 2.41 (s, 3H, CH3), 6.30 (d, J = 7.8 Hz, 1H, furyl-H), 6.60 (d, J = 7.8 Hz, 1H, furyl-H), 7.38 (s, 1H, CH=C), 7.20 (d, J = 8.2 Hz, 2H, Ar-H), 7.36 (d, J = 8.2 Hz, 2H, Ar-H); 13C-NMR: 21.4 (CH3), 24.1–39.37 (5CH2), 73.35 (spiro-C), 116.4–160.1 (Ar-C and methine-C and thiazolidine-C5, 167.9 (C=O); MS, m/z (%): 357.12 (M+, 26.5). Anal. calc. for C20H20FNO2S (357.44): C, 67.21; H, 5.64; N, 3.92. Found: C, 67.02; H, 5.51; N, 3.69.

3.1.3. 3′-(4-Fluorophenyl)-7′-(5-methylfuran-2-yl)-3′H-spiro[cyclohexane-1,2′-thiazolo[4,5-d]pyrimidine]-5′(6′H)-thione (5)

A mixture of the compound of the arylidine 4 (0.01 mol, 3.57 g) and thiourea (0.01 mol, 0.76 g) was fused in the presence of 10 drops of piperidine in an oil bath for 3 h at 180 °C. The product was poured onto crushed ice; the solid was filtered off and recrystallized from acetic acid to give compound 5.
Brownish powder; Yield: 74%; m.p. 140–141 °C; IR (KBr, υ, cm−1): 3241 (NH), 3045 (C-H aromatic), 2940 (C-H aliphatic), 1612 (C=N); 1H-NMR (DMSO-d6): δ (ppm) 1.64–1.96 (m, 10H, 5CH2), 2.46 (s, 3H, CH3), 6.70 (d, J = 7.8 Hz, 1H, furyl-H), 6.90 (d, J = 7.8 Hz, 1H, furyl-H), 7.26 (d, J = 8.2 Hz, 2H, Ar-H), 7.60 (d, J = 8.2 Hz, 2H, Ar-H), 13.25 (brs, 1H, NH); 13C-NMR: 21.4 (CH3), 23.8–39.4 (5CH2), 73.35 (spiro-C), 116.38–161.4 (Ar-C and pyrimidine-C5,6), 165.2 (C=N), 182.13 (C=S); MS, m/z (%): 413.2 (M+, 1.37). Anal. calc. for C21H20FN3OS2 (413.53): C, 60.99; H, 4.88; N, 10.16. Found: C, 60.72; H, 4.75; N, 9.90.

3.1.4. 3′-(4-Fluorophenyl)-7′-(5-methylfuran-2-yl)-5′-(O-acetyl-d-glycopyranosyl)-3′H-spiro[cyclohexane-1,2′-thiazolo[4,5-d]pyrimidine] (7,8)

To a well stirred solution of thione derivative 5 (0.005 mol, 2.65 g) in aqueous potassium hydroxide [0.005 mol in distilled water (2 mL)] was added a solution of 2,3,4,6-tetra-O-acetyl-α-d-gluco- or 2,3,4,-tri-O-acetyl-α-d-xylopyranosyl bromide (6a,b) (0.005 mol) in acetone (20 mL)portion wise. The reaction mixture was stirred at room temperature for 6 h at which TLC (pet. ether/EtOAc 3:1) showed completion of the glycosylation. The solvent was evaporated under reduced pressure and the residue was washed with distilled water to afford a product which was dried and crystallized from ethanol to give compound 7 and 8, respectively.
3′-(4-Fluorophenyl)-7′-(5-methylfuran-2-yl)-5′-(2,3,4,6-tetra-O-acetyl-d-glucopyranosyl)-3′H-spiro[cyclohexane-1,2′-thiazolo[4,5-d]pyrimidine] (7). Brownish powder, Yield: 76%; m.p. 129–130 °C; IR (KBr, υ, cm−1): 3055 (C-H aromatic), 2950 (C-H aliphatic), 1748 (C=O), 1614 (C=N); 1H-NMR (DMSO-d6): δ (ppm) 1.51–1.91 (m,10H, 5CH2), 1.97, 2.06, 2.11, 2.18, 2.35 (5s, 15H, 5CH3), 3.70–3.75 (m, 1H, H-5′), 4.02–4.07 (dd, J = 3.8, 10.2 Hz, 1H, H-6′′), 4.15–4.19 (dd, J = 11.3, 3.8 Hz, 1H, H-6′), 4.60 (t, J = 8.6 Hz, 1H, H-4′), 4.95 (dd, J = 9.8, J = 8.6 Hz, 1H, H-2′), 5.33 (d, J = 9.8 Hz, 1H, H-1′), 5.65 (t, J = 9.8 Hz, 1H, H-3′), 6.15 (d, J = 7.8 Hz, 1H, furyl-H), 6.60 (d, J = 7.8 Hz, 1H, furyl-H), 7.11–7.34 (m, 4H, Ar-H); 13C-NMR (DMSO-d6): δ (ppm) 14.1, 20.7, 20.8, 20.9, 21.0 (5 CH3), 27.1, 31.5, 38.2, 39.3 (cyclohexyl-C), 62.1 (C-5), 62.3 (C-6), 69.3 (C-4), 71.1 (C-3), 72.8 (spiro-C), 78.2 (C-2), 89.3 (C-1), 109.1–156.6 (Ar-C), 162.6 (pyrimidine-C2), 169.5, 169.8, 170.5, 172.5 (4 C=O). Analysis calc. for C35H38FN3O10S2 (743.82): C, 56.52; H, 5.15; N, 5.65. Found: C, 56.29; H, 5.05; N, 5.88.
3′-(4-Fluorophenyl)-7′-(5-methylfuran-2-yl)-5′-(2,3,4-tri-O-acetyl-d-xylopyranosyl)-3′H-spiro[cyclohexane-1,2′-thiazolo[4,5-d]pyrimidine] (8). Brownish powder, Yield: 72%; m.p. 133–134 °C; IR (KBr, υ, cm−1): 3045 (C-H aromatic), 2950 (C-H aliphatic), 1745 (C=O), 1615 (C=N); 1H-NMR (DMSO-d6): δ (ppm) 1.50–1.92 (m, 10H, 5CH2), 1.98, 2.05, 2.14, 2.34 (4s, 12H, 4CH3),4.12–4.16 (dd, J = 3.8, 10.2 Hz, 1H, H-5′′), 4.20–4.25 (dd, J = 11.3, 3.8 Hz, 1H, H-5′), 4.69 (m, 1H, H-4′), 5.05 (dd, J = 5.2, 8.8 Hz, 1H, H-2′), 5.33 (d, J = 9.6 Hz, 1H, H-1′), 5.59 (t, J = 8.8 Hz, 1H, H-3′), 6.17 (d, J = 7.8 Hz, 1H, furyl-H), 6.62 (d, J = 7.8 Hz, 1H, furyl-H), 7.15–7.38 (m, 4H, Ar-H); 13C-NMR (DMSO-d6): δ (ppm) 14.9, 20.2, 20.7, 20.9 (4 CH3), 27.5, 27.7, 40.6, 41.5 (cyclohexyl-C), 61.9 (C-5), 67.5 (C-4), 70.6 (C-3), 73.6 (spiro-C), 73.8 (C-2), 89.3 (C-1), 111.9–153.2 (Ar-C), 163.4 (pyrimidine-C2), 168.6, 169.9, 170.4 (3 C=O). Analysis calc. for C32H34FN3O8S2 (671.76): C, 57.22; H, 5.10; N, 6.26. Found: C, 57.51; H, 5.29 N, 6.11.

3.1.5. 3′-(4-Fluorophenyl)-7′-(5-methylfuran-2-yl)-5′-(d-glycopyranosyl)-3′H-spiro[cyclohexane-1,2′-thiazolo[4,5-d]pyrimidine] (9,10)

The acetylated glycoside 7 or 8 (0.01 mol) was dissolved in dry methanol saturated with ammonia gas (20 mL) at 0 °C and the solution was stirred at room temperature for 8 h. The solvent was evaporated under reduced pressure at 40 °C and the residue was treated with diethyl ether (15 mL) to afford compound 9 or 10, respectively.
5′-(d-Glucopyranosyl)-3′-(4-fluorophenyl)-7′-(5-methylfuran-2-yl)-3′H-spiro[cyclohex-ane-1,2′-thiazolo[4,5-d]pyrimidine] (9) Brownish powder, Yield: 70%; m.p. 143–145 °C; IR (KBr, υ, cm−1): 3488–3390 (OH), 3038 (C-H aromatic), 2948 (C-H aloiphatic), 1610 (C=N). 1H-NMR (DMSO-d6, δ, ppm): 1.55–1.92 (m, 10H, 5CH2), 2.31 (s, 3H, CH3),3.47–3.75 (m, 2H, H-6′, H-6′′), 3.90–4.07 (m, 1H, H-5′), 4.15–4.119 (m, 1H, H-4′), 4.72–4.89 (m, 3H, H-3′, H-2′, OH), 5.12–5.18 (m, 2H, 2OH), 5.40 (m, 1H, OH), 5.77 (d, J = 9.8 Hz, 1H, H-1′), 6.15 (d, J = 7.8 Hz, 1H, furyl-H), 6.60 (d, J = 7.8 Hz, 1H, furyl-H), 7.15 (d, J = 8.4 Hz, 2H, Ar-H), 7.38 (d, J = 8.4 Hz, 2H, Ar-H); Analysis calc. for C27H30FN3O6S2 (575.67): C, 56.33; H, 5.25; N, 7.30. Found: C, 56.02; H, 5.05; N, 7.61.
3′-(4-Fluorophenyl)-7′-(5-methylfuran-2-yl)-5′-(d-xylo)-3′H-spiro[cyclohexane-1,2′-thiazolo[4,5-d]pyrimidine] (10). Brownish powder, Yield: 69%; m.p. 143–145 °C; IR (KBr, υ, cm−1): 3475–3405 (OH), 3060 (C-H aromatic), 2935 (C-H aloiphatic), 1612 (C=N). 1H-NMR (DMSO-d6, δ, ppm): 1.54–1.93 (m, 10H, 5CH2), 2.32 (s, 3H, CH3), 3.59–3.79 (m, 2H, H-5′, H-5′′), 4.22–4.27 (m, 1H, H-4′), 4.75–4.92 (m, 3H, H-3′, H-2′, OH), 5.12–5.18 (m, 1H, OH), 5.35 (m, 1H, OH), 5.79 (d, J = 9.8 Hz, 1H, H-1′), 6.18 (d, J = 7.8 Hz, 1H, furyl-H), 6.61 (d, J = 7.8 Hz, 1H, furyl-H), 7.16 (d, J = 8.4 Hz, 2H, Ar-H), 7.39 (d, J = 8.4 Hz, 2H, Ar-H); Analysis calc. for C26H28FN3O5S2 (545.64): C, 57.23; H, 5.17; N, 7.70. Found: C, 57.44; H, 5.27; N, 7.92.

3.1.6. Synthesis of Aryl 1-Thia-4-azaspiro[4.5]decan-3-ylidene)amino)-1,3,4-thiadiazole-2-thiol (12,13)

To a solution of compound 2 or 3 (0.01 mol) and 2-amino-1,3,4-thiadiazole-5-thiol (11) (0.01 mol) in absolute ethanol (30 mL) was added 2 mL of glacial acetic acid. The reaction mixture was refluxed for 5 h, then left to cool; the formed solid was filtered off, washed with water, and crystallized to give compounds 12 or 13, respectively.
5-((4-(4-Fluorophenyl)-1-thia-4-azaspiro[4.5]decan-3-ylidene)amino)-1,3,4-thiadiazole-2(3H)-thione (12). Pale brown powder, Yield: 71%; m.p. 156–157 °C; IR (KBr, υ, cm−1): 3250 (NH), 3055 (C-H aromatic), 2930 (C-H aliphatic), 1610 (C=N). 1H-NMR (DMSO-d6, δ, ppm): 1.45–1.95 (m, 10H, 5CH2), 3.60 (s, 2H, CH2), 7.13–7.60 (m, 4H, Ar-H), 13.25 (s, 1H, NH); 13C-NMR: 23.4, 28.2, 32.0, 39.1 (cyclohexyl-C and thiadiazole CH2), 74.05 (spiro-C), 116.4–132.2 (Ar-C), 161.6–162.5 (thiazole-C4 and thiadiazole-C), 182.13 (C=S); MS, m/z (%): 381 (M+ + 1, 22); Analysis calc. for C16H17FN4S3 (380.52): C, 50.50; H, 4.50; N, 14.72. Found: C, 50.28; H, 4.37; N, 14.98.
5-((4-(4-Fluorophenyl)-8-methyl-1-thia-4-azaspiro[4.5]decan-3-ylidene)amino)-1,3,4-thiadiazole-2(3H)-thione (13). Yellow powder, Yield: 71%; m.p. 145–146 °C; IR (KBr, υ, cm−1): 3275 (NH), 3050 (C-H aromatic), 2938 (C-H aliphatic), 1612 (C=N). 1H-NMR (DMSO-d6, δ, ppm): 0.82 (s, 3H, CH3), 1.52–1.99 (m, 9H, cyclohexyl-H), 3.65 (s, 2H,CH2), 7.06–7.32 (m, 4H, Ar-H), 13.11 (s, 1H, NH); 13C-NMR: 15.3 (CH3), 22.7, 32.5, 33.6, 39.2 (cyclohexyl-C and thiadiazole CH2), 74.1 (spiro-C), 112.3–148.9 (Ar-C), 162.6–163.1 (thiazole-C4 and thiadiazole-C), 182.1 (C=S); MS, m/z (%): 394 (M+, 18); Analysis calc. for C17H19FN4S3 (394.55): C, 51.75; H, 4.85; N, 14.20. Found: C, 51.49; H, 4.77; N, 14.03.

3.1.7. Synthesis of N-(5-(Acetylated sugar)-1,3,4-thiadiazol-2-yl)-4-(4-fluorophenyl)-1-thia-4-azaspiro[4.5]decan-3-imine (14–17)

A stirred solution of 2,3,4,6-tetra-O-acetyl-α-d-gluco- or 2,3,4-tri-O-acetyl-d-xylopyranosyl bromide (0.005 mol) in acetone (20 mL) was added portion wise to a solution of thione derivative 12 or 13 (0.005 mol) in aqueous potassium hydroxide [0.01 mol in distilled water (2 mL)]. The resulting mixture was stirred at room temperature for 5–6 h (TLC, Pet. ether/EtOAc 3:1). The solvent was evaporated under reduced pressure then ice cold water was added to the residue with stirring for 1 h. It was then left to stand overnight in a refrigerator to afford a product which was dried and crystallized from ethanol to give compound 14–17.
4-(4-Fluorophenyl)-N-(5-(2,3,4,6-tetra-O-acetyl-d-glucopyranosyl)-1,3,4-thiadiazol-2-yl)-1-thia-4-azaspiro[4.5]decan-3-imine (14). Brownish solid, Yield: 68%, m.p. 138–139 °C; IR (KBr, cm−1): 3062 (C-H aromatic), 2936 (C-H aliphatic), 1752 (C=O), 1618 (C=N). 1H-NMR (DMSO-d6, δ, ppm): 1.28–1.93 (m, 10H, 5CH2), 1.96, 1.97, 1.99, 2.02 (4s, 12H, 4CH3), 3.54 (s, 2H, CH2), 3.67–3.71 (m, 1H, H-5′), 4.01–4.04 (dd, 1H, J = 3.3, J = 11.6 Hz, H-6′), 4.18–4.21 (dd, 1H, J = 11.6, J = 2.8 Hz, H-6′′), 4.35 (m, 1H, H-4′), 4.90–4.96 (t, 1H, J = 10.8 H-2′), 5.29 (d, 1H, J = 10.8 Hz, H-1′), 5.34 (t, 1H, J = 9.2 Hz, H-3′), 7.12–7.27 (m, 4H, Ar-H); 13C-NMR (DMSO-d6): 20.2, 20.7, 20.9, 21.1 (4 CH3), 23.5, 28.9, 32.0, 38.1 (cyclohexyl-C and thiadiazole CH2), 61.7 (C-5), 67.8 (C-6), 69.1 (C-4), 70.5 (C-3), 72.9 (spiro-C), 73.6 (C-2), 91.2 (C-1), 116.2–152.2 (Ar-C), 162.9–163.2 (thiazole-C4 and thiadiazole-C), 169.4, 169.7, 170.0, 170.5 (4 C=O). Analysis calc. for C30H35FN4O9S3 (710.81): C, 50.69; H, 4.96; N, 7.88. Found: C, 50.49; H, 4.82; N, 7.60.
4-(4-Fluorophenyl)-N-(5-(2,3,4,6-tri-O-acetyl-d-xylopyranosyl)-1,3,4-thiadiazol-2-yl)-1-thia-4-azaspiro[4.5]decan-3-imine (15). Gray powder Yield: 74%, m.p. 145–146 °C; IR (KBr, cm−1): IR (KBr, cm−1): 3048 (C-H aromatic), 2935 (C-H aliphatic), 1752 (C=O), 1612 (C=N); 1H-NMR (DMSO-d6, δ, ppm): 1.26–1.94 (m, 10H, 5CH2), 1.97, 1.99, 2.02 (3s, 9H, 3CH3), 3.52 (s, 2H, CH2), 4.04–4.14 (m, 2H, H-5′′, H-5′), 4.49–4.52 (dd, 1H, J = 6.6 Hz, J = 7.8 Hz, H-4′), 4.99–5.02 (dd, 1H, J = 7.8, J = 10.2 Hz Hz, H-2′), 5.29 (d, 1H, J = 10.2 Hz, H-1′), 5.46 (t, 1H, J = 7.8 Hz, H-3′), 7.27 (m, 4H, Ar-H); Analysis calc. for C27H31FN4O7S3 (638.74): C, 50.77; H, 4.89; N, 8.77; Found: C, 50.48; H, 4.69; N, 8.60.
4-(4-Fluorophenyl)-N-(5-(2,3,4,6-tetra-O-acetyl-d-glucopyranosyl)-1,3,4-thiadiazol-2-yl)-8-methyl-1-thia-4-azaspiro[4.5]decan-3-imine (16). Brownish solid, Yield: 65%, m.p. 136–137 °C; IR (KBr, cm−1): 3055 (C-H aromatic), 2940 (C-H aliphatic), 1748 (C=O), 1616 (C=N). 1H-NMR (DMSO-d6, δ, ppm): 0.83 (d, 3H, J = 5.8 Hz, CH3), 1.28–1.89 (m, 9H, cyclohexyl-H), 1.95, 1.97, 2.00, 2.06 (4s, 12H, 4CH3), 3.50 (s, 2H, CH2), 3.71–3.75 (m, 1H, H-5′), 4.15–4.19 (m, 1H, H-6′′), 4.25–4.31 (dd, 1H, J = 3.4, J = 10.8 Hz, H-6′), 4.40 (dd, 1H, J = 8.2 Hz, J = 9.8 Hz, H-4′), 5.08 (t, 1H, J = 10.8 H-2′), 5.31 (d, 1H, J = 10.4 Hz, H-1′), 5.66 (t, 1H, J = 9.2 Hz, H-3′), 7.11–7.23 (m, 4H, Ar-H); 13C-NMR (DMSO-d6): 17.7, 20.8, 20.8, 21.1, 21.1 (5 CH3), 27.3, 31.1, 38.5, 38.7 (cyclohexyl-C and CH2), 62.1 (C-5), 67.7 (C-6), 68.8 (C-4), 71.1 (C-3), 72.8 (spiro-C), 73.8 (C-2), 89.2 (C-1), 116.3–152.2 (Ar-C), 162.1–163.4 (thiazole-C4 and thiadiazole-C), 169.5, 169.7, 170.5, 171.8 (4 C=O). Analysis calc. for C31H37FN4O9S3 (724.83): C, 51.37; H, 5.15; N, 7.73. Found: C, 51.05; H, 4.96; N, 7.98.
4-(4-Fluorophenyl)-N-(5-(2,3,4,6-tri-O-acetyl-d-xylopyranosyl)-1,3,4-thiadiazol-2-yl)-8-methyl-1-thia-4-azaspiro[4.5]decan-3-imine (17). Pale yellow powder, Yield: 75%, m.p. 136–137 °C; IR (KBr, cm−1): 3040 (C-H aromatic), 2938 (C-H aliphatic), 1750 (C=O), 1617 (C=N); 1H-NMR (DMSO-d6, δ, ppm): 0.89 (d, 3H, J = 5.6 Hz, CH3), 1.29–1.95 (m, 9H, cyclohexyl-H), 1.99, 2.03, 2.05 (3s, 9H, 3CH3), 3.53 (s, 2H, CH2), 4.02–4.14 (m, 2H, H-5′′, H-5′), 4.76–4.79 (dd, 1H, J = 7.0 Hz, J = 7.6 Hz, H-4′), 5.15 (dd, 1H, J = 7.5, J = 9.8 Hz, H-2′), 5.30 (d, 1H, J = 9.8 Hz, H-1′), 5.39 (t, 1H, J = 7.5 Hz, H-3′), 7.14–7.27 (m, 4H, Ar-H); 13C-NMR (DMSO-d6): 17.0, 20.9, 21.1, 21.9 (4 CH3), 24.5, 28.8, 32.8, 38.4 (cyclohexyl-C and CH2), 67.8 (C-5), 68.1 (C-4), 70.4 (C-3), 73.6 (spiro-C), 74.1 (C-2), 89.5 (C-1), 116.4–154.9 (Ar-C), 160.9–163.4 (thiazole-C4 and thiadiazole-C), 169.7, 170.4, 171.8 (3 C=O); Analysis calc. for C28H33FN4O7S3 (652.77): C, 51.52; H, 5.10; N, 8.58. Found: C, 51.29; H, 5.02; N, 8.39.

3.1.8. 4-(4-Fluorophenyl)-N-(5-(d-glycopyranosyl)-1,3,4-thiadiazol-2-yl)-8-methyl-1-thia-4-azaspiro[4.5]decan-3-imine (18,19)

The acetylated 1,3,4-thiadiazolyl glycoside 16 or 17 ((0.005 mol)) was dissolved with stirring in anhydrous methanol saturated with ammonia gas (20 mL) at 0 °C and the solution was then further stirred at room temperature for 7 h. The solvent was evaporated under reduced pressure at 40 °C, then a mixture of diethyl ether and pet. ether (1:1) was added to the residue with stirring for about 30 min. The formed solid substance was filtered, washed with cold ethanol and crystallized to give 18 or 19, respectively.
4-(4-Fluorophenyl)-N-(5-(d-glucopyranosyl)-1,3,4-thiadiazol-2-yl)-8-methyl-1-thia-4-azaspiro[4.5]decan-3-imine (18). Pale yellow powder, Yield: 69%, m.p. 180–181 °C; IR (KBr, cm−1): 3483–3428 (OH), 3058 (C-H aromatic), 2925 (C-H aliphatic), 1615 (C=N); 1H-NMR (DMSO-d6, δ, ppm): 0.88 (d, 3H, J = 5.8 Hz, CH3),1.47–2.07 (m, 9H, cyclohexyl-H), 3.43–3.51 (m, 4H, H-6′′, H-6′ and CH2), 3.64–3.69 (m, 1H, H-5′), 3.75–4.18 (m, 3H, H-4′, H-3′ and OH), 4.21–4.23 (m, 1H, OH), 5.11 (m, 2H, OH and H-2′), 5.33 (m, 1H, OH), 5.65 (d, 1H, J = 9.8 Hz, H-1′), 7.16–7.61 (m, 4H, Ar-H); 13C-NMR (DMSO-d6): 16.4 (CH3), 25.9, 28.8, 39.1 (cyclohexyl-C and CH2), 62.1 (C-6), 67.8 (C-5), 68.9 (C-4), 71.1 (C-3), 72.5 (spiro-C), 78.2 (C-2), 90.2 (C-1), 109.2–154.2 (Ar-C), 160.5–163.1 (thiazole-C4 and thiadiazole-C); Analysis calc. for C23H29FN4O5S3 (556.69): C, 49.62; H, 5.25; N, 10.06. Found: C, 49.89; H, 4.99; N, 9.95.
4-(4-Fluorophenyl)-N-(5-(d-xylopyranosyl)-1,3,4-thiadiazol-2-yl)-8-methyl-1-thia-4-azaspiro[4.5]decan-3-imine (19). Pale yellow powder, Yield: 63%, m.p.170–171 °C; IR (KBr, cm−1): 3495–3446 (OH), 3060 (C-H aromatic), 2918 (C-H aliphatic), 1619 (C=N); 1H-NMR (DMSO-d6, δ, ppm): 0.89 (d, 3H, J = 5.8 Hz, CH3), 1.47–2.14 (m, 9H, cyclohexyl-H), 3.39–3.44 (m, 2H, H-5′′, H-5′), 3.52 (s, 2H, CH2), 3.65–3.88 (m, 2H, H-4′, H-3′), 4.23–4.27 (m, 2H, 2OH), 4.90–5.12 (m, 2H, H-2′, OH), 5.65 (d, 1H, J = 9.8 Hz, H-1′), 6.87–7.58 (m, 4H, Ar-H); Analysis calc. for C22H27FN4O4S3 (526.66): C, 50.17; H, 5.17; N, 10.64. Found: C, 49.98; H, 5.02; N, 10.50.

3.2. Anticancer Screening

3.2.1. Cells

Cell culture of HepG-2 (human liver carcinoma), PC-3 (human prostate adenocarcinoma) and HCT116 (human colorectal carcinoma) cell lines were purchased from the American Type Culture Collection (Rockville, MD, USA) and maintained in Roswell Park Memorial Institute (RPMI-1640) medium which was supplemented with 10% heat-inactivated FBS (fetal bovine serum), 100 U/mL penicillin and 100 U/mL streptomycin. The cells were grown at 37 °C in a humidified atmosphere of 5% CO2.

3.2.2. MTT Cytotoxicity Assay

The antitumor activity against liver HepG-2, prostate PC-3 and colon HCT-116 human cancer cell lines was estimated using the 3-[4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay, which is based on the cleavage of the tetrazolium salt by mitochondrial dehydrogenases in viable cells [44,45,46]. Cells were dispensed in a 96-well sterile microplate (5 × 104 cells/well), and incubated at 37 °C with series of different concentrations, in DMSO, of each tested compound or Doxorubicin® (positive control) for 48 h in a serum-free medium prior to the MTT assay. After incubation, media were carefully removed, 40 µL of MTT (2.5 mg/mL) was added to each well and then incubated for an additional 4 h. The purple formazan dye crystals were solubilized by the addition of 200 µL of DMSO. The absorbance was measured at 590 nm using a SpectraMax® Paradigm® Multi-Mode microplate reader. The relative cell viability was expressed as the mean percentage of viable cells compared to the untreated control cells.

3.2.3. Statistical Analysis

All experiments were conducted in triplicate and repeated on three different days. All the values were represented as mean ± SD. IC50s were determined by probit analysis using the SPSS software program (version 20, SPSS Inc., Chicago, IL, USA).

4. Conclusions

The thioglycoside derivatives of novel 1,3,4-thiadiazole and thiazolopyrimidine compounds incorporating 1-thia-azaspiro[4.5]decane ring system were synthesized. The anticancer activity against human liver hepatocellular carcinoma (HepG-2), human prostate adenocarcinoma (PC-3) and human colorectal carcinoma (HCT-116) cell lines was investigated. A number of compounds showed moderate to high anticancer activity and the results revealed the importance of attachment of glycosyl moieties to the thiazolopyrimidine or (1,3,4-thiadiazolyl)thiazolidinone ring systems.

Acknowledgments

Authors would like to thank and acknowledge Taibah University for the support in the publication process of the research.

Author Contributions

The research group from the National Research Centre, Dokki, Giza, Egypt including Wael A. El-Sayed, Walaa I. El-Sofany, Eman M. Flefel (Photochemistry Department) and Ashraf M. Mohamed (Applied Organic Chemistry Department) conceived the research project, participated in the research steps, interpreted the results, discussed the experimental data and prepared the manuscript. Hanem M. Awad (Department of Tanning Materials and Leather Technology, National Research Centre) conducted the biological assays and provided the experimental procedures and results of biological part.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. De Martino, J.K.; Boger, D.L. Glycinamide ribonucleotide transformylase (GAR TFase) as a target for cancer therapy. Drug Future 2008, 33, 969–979. [Google Scholar] [CrossRef]
  2. Curran, W.J. New chemotherapeutic agents: Update of major chemoradiation trials in solid tumors. Oncology 2002, 63, 29–38. [Google Scholar] [CrossRef] [PubMed]
  3. El-Sayed, W.A.; Abd El-Monem, Y.A.; Yousif, N.M.; Tawfek, N.; Shaaban, M.T.; Abdel Rahman, A.A.-H. Antimicrobial Activity of New 2,4-Disubstituted Thiazolidinone Derivatives. Z. Naturforsch. 2009, 64C, 785–790. [Google Scholar] [CrossRef]
  4. Mishra, R.; Tomar, I.; Singhal, S.; Jha, K.K. Facile synthesis of thiazolidinones bearing thiophene nucleus as antimicrobial agents. Der. Pharm. Chem. 2012, 4, 489–496. [Google Scholar]
  5. Patela, D.; Patela, R.; Kumaria, P.; Patelb, N.B. Synthesis of s-triazine-based thiazolidinones as antimicrobial agents. Z. Naturforsch. 2012, 67c, 108–122. [Google Scholar] [CrossRef]
  6. Abhinit, M.; Ghodke, M.; Pratima, N.A. Exploring potential of 4-thiazolidinone: A brief review. Int. J. Pharm. Pharm. Sci. 2009, 1, 47–64. [Google Scholar]
  7. Balzarini, J.; Orzeszko, B.; Maurin, J.K.; Orzeszko, A. Synthesis and anti-HIV studies of 2-adamantyl-substituted thiazolidin-4-ones. Eur. J. Med. Chem. 2007, 42, 993–1003. [Google Scholar] [CrossRef] [PubMed]
  8. Li, W.; Lu, Y.; Wang, Z.; Dalton, J.T.; Miller, D.D. Synthesis and antiproliferative activity of thiazolidine analogs for melanoma. Bioorg. Med. Chem. Lett. 2007, 17, 4113–4117. [Google Scholar] [CrossRef] [PubMed]
  9. Chandrappa, S.; Benaka Prasad, S.B.; Vinaya, K.; Ananda Kumar, C.S.; Thimmegowda, N.R.; Rangappa, K.S. Synthesis and in vitro antiproliferative activity against human cancer cell lines o novel 5-(4-methyl-benzylidene)-thiazolidine-2,4-diones. Investig. New Drugs 2008, 26, 437–444. [Google Scholar] [CrossRef] [PubMed]
  10. Anh, H.L.T.; Cuc, N.T.; Tai, B.H.; Yen, P.H.; Nhiem, N.X.; Thao, D.T.; Na, N.H.; Minh, C.V.; Kiem, P.V.; Kim, Y.H. Synthesis of Chromonylthiazolidines and Their Cytotoxicity to Human Cancer Cell Lines. Molecules 2015, 20, 1151–1160. [Google Scholar] [CrossRef] [PubMed]
  11. Joshi, H.; Pal, T.; Ramaa, C.S. A new dawn for the use of thiazolidinediones in cancer therapy. Expert Opin. Investig. Drugs 2014, 23, 501–510. [Google Scholar] [CrossRef] [PubMed]
  12. Blanquicett, C.; Roman, J.; Hart, C.M. Thiazolidinediones as anti-cancer agents. Cancer Ther. 2008, 6, 25–34. [Google Scholar] [PubMed]
  13. Chen, X.; Chong, C.R.; Shi, L.; Yoshimoto, T.; Sullivan, D.J.; Liu, J.O. Inhibitors of Plasmodium falciparum methionine aminopeptidase 1b possess antimalarial activity. Proc. Natl. Acad. Sci. USA 2006, 103, 14548–14553. [Google Scholar] [CrossRef] [PubMed]
  14. Flefel, E.M.; Sayed, H.H.; Hashem, A.I.; Shalaby, E.A.; El-Sofany, W.; Abdel-Megeid, F.M.E. Pharmacological evaluation of some novel synthesized compounds derived from spiro(cyclohexane-1,20-thiazolidines). Med. Chem. Res. 2014, 23, 2515–2527. [Google Scholar] [CrossRef]
  15. Amr, A.-E.-G.; Maigali, S.S.; Abdulla, M.M. Synthesis, and analgesic and antiparkinsonian activities of thiopyrimidine, pyrane, pyrazoline, and thiazolopyrimidine derivatives from 2-chloro-6-ethoxy-4-acetylpyridine. Monatsh. Chem. 2008, 139, 1409–1415. [Google Scholar] [CrossRef]
  16. Branstetter, B.J.; Breitenbucher, J.G.; Lebsack, A.D.; Xiao, W. Thiazolopyrimidine Modulators of TRPV1. U.S. Patent WO 005303, 10 January 2008. [Google Scholar]
  17. Mohamed, A.M.; Amr, A.E.G.; Alsharari, M.A.; Al-Qalawi, H.R.M.; Germoush, M.O.; Al-Omar, M.A. Anticancer Activities of Some New Synthesized Thiazolo[3,2-a]Pyrido[4,3-d]Pyrimidine Derivatives. Am. J. Biochem. Biotechnol. 2011, 7, 43–54. [Google Scholar] [CrossRef]
  18. Hammam, A.G.; El-Salam, O.I.A.; Mohamed, A.M.; Hafez, N.A. Novel fluoro substituted benzo[o]pyranwith anti-lung cancer activity. Ind. J. Chem. 2005, 44B, 1887–1893. [Google Scholar]
  19. Flefel, E.E.; Salama, M.A.; El-Shahat, M.; El-Hashash, M.A.; El-Farargy, A.F. A novel synthesis of some new pyrimidine and thiazolopyrimidine derivatives for anticancer evaluation. Phosphorus Sulfur Silicon Relat. Elem. 2007, 182, 1739–1756. [Google Scholar] [CrossRef]
  20. Youssef, M.M.; Amin, M.A. Microwave Assisted Synthesis of Some New Thiazolopyrimidine, Thiazolodipyrimidine and Thiazolopyrimidothiazolopyrimidine Derivatives with Potential Antioxidant and Antimicrobial Activity. Molecules 2012, 17, 9652–9667. [Google Scholar] [CrossRef] [PubMed]
  21. Said, M.; Abouzid, K.; Mouneer, A.; Ahmedy, A.; Osman, A.-M. Synthesis and biological evaluation of new thiazolopyrimidines. Arch. Pharm. Res. 2004, 27, 471–477. [Google Scholar] [CrossRef] [PubMed]
  22. Linder, W.; Brandes, W. Pesticidal Thiazolopyrimidine Derivatives. U.S. Patent 4996208, 26 February 1991. [Google Scholar]
  23. Duval, R.; Kolb, S.; Braud, E.; Genest, D.; Garbay, C. Rapid discovery of triazolobenzylidenethiazolopyrimidines (TBTP) as CDC25 phosphatase inhibitors by parallel click chemistry and in situ screening. J. Comb. Chem. 2009, 11, 947–950. [Google Scholar] [CrossRef] [PubMed]
  24. Kolb, S.; Mondésert, O.; Goddard, M.L.; Jullien, D.; Villoutreix, B.O.; Ducommun, B.; Garbay, C.; Braud, E. Development of novel thiazolopyrimidines as CDC25B phosphatase inhibitors. ChemMedChem 2009, 4, 633–648. [Google Scholar] [CrossRef] [PubMed]
  25. Zhi, H.; Chen, L.; Zhang, L.; Liu, S.; Wan, D.C.C.; Lin, H.; Hu, C. Design, synthesis, and biological evaluation of 5H-thiazolo[3,2-a]pyrimidine derivatives as a new type of acetylcholinesterase inhibitors. ARKIVOC 2008, xiii, 266–277. [Google Scholar]
  26. Rashad, A.E.; Shamroukh, A.H.; Abdel-Megeid, R.E.; El-Sayed, W.A. Synthesis, reactions and antimicrobial evaluation of some polycondensedthieno-pyrimidine derivatives. Synth. Commun. 2010, 40, 1149–1160. [Google Scholar] [CrossRef]
  27. El-Emary, T.I.; Abdel-Mohsen, S.A. Synthesis and antimicrobial activity of some new 1,3-diphenylpyrazoles bearing pyrimidine, Pyrimidinethione, thiazolopyrimidine, triazolopyrimidine, thio- and alkylthiotriazolopyrimidinone moieties at the 4-position. Phosphorus Sulfur 2006, 181, 2459–2474. [Google Scholar] [CrossRef]
  28. Maddila, S.; Damu, G.L.V.; Oseghe, E.O.; Abafe, O.A.; Venakata, R.C.; Lavanya, P. Synthesis and biological studies of novel biphenyl-3,5-dihydro-2H-thiazolo-pyrimidines derivatives. J. Korean Chem. Soc. 2012, 56, 334–340. [Google Scholar] [CrossRef]
  29. Fahmy, H.T.Y.; Rostom, S.A.F.; Saudi, M.N.; Zjawiony, J.K.; Robins, D.J. Synthesis and in vitro evaluation of the anticancer activity of novel fluorinated thiazolo[4,5-d]pyrimidines. Arch. Pharm. Pharm. Med. Chem. 2003, 336, 216–225. [Google Scholar] [CrossRef] [PubMed]
  30. Dawood, K.M.; Gomha, S.M. Synthesis and Anti-cancer Activity of 1,3,4-Thiadiazole and 1,3-Thiazole Derivatives Having 1,3,4-Oxadiazole Moiety. J. Heterocycl. Chem. 2015, 52, 1400–1405. [Google Scholar] [CrossRef]
  31. Blanc-Muesser, M.; Vigne, L.; Driguez, H.; Lehmann, J.; Steck, J.; Urbahns, K. Spacer-modified disaccharide and pseudo trisaccharide methyl glycosides that mimic maltotriose, as competitive inhibitors for pancreatic alpha-amylase: A demonstration of the “clustering effect”. Carbohydr. Res. 1992, 224, 59–71. [Google Scholar] [CrossRef]
  32. El Ashry, E.S.H.; Awad, L.F.; Atta, I.A. Synthesis and role of glycosylthio heterocycles in carbohydrate chemistry. Tetrahedron 2006, 62, 2943–2998. [Google Scholar] [CrossRef]
  33. El-Sayed, W.A.; Abbas, H.S.; Abdel Magid, R.E.; Magdziarz, T. Synthesis, Antimicrobial Activity and Docking Studies of New 3-(Pyrimidin-4-yl)-1H-indole Derivatives and Their Derived N- and S-glycoside Analogs. Med. Chem. Res. 2016, 25, 339–355. [Google Scholar] [CrossRef]
  34. Mereyala, H.B.; Gurijala, V.R. Use of 2-pyridyl-2-acetamido-3,4,6-tri-O-acetyl-2- deoxy-1-thio-b-d-gluco-pyranoside as a glycosyl donor and methyl iodide as anactivator for the synthesis of 1,2-trans-linked saccharides. Carbohydr. Res. 1993, 242, 277–280. [Google Scholar] [CrossRef]
  35. Abbas, H.S.; El-Sayed, W.A.; Fathy, N.M. Synthesis and antitumor activity of new dihydropyridine thioglycosides and their corresponding dehydrogenated forms. Eur. J. Med. Chem. 2010, 45, 973–982. [Google Scholar] [CrossRef] [PubMed]
  36. Mohamed, A.M.; El-Sayed, W.A.; Al-Qallawi, H.R.M.; Germoush, M.O. Synthesis and antimicrobial activity of New Norbornyl system based Oxadiazole Thioglycosides and Acyclic Nucleoside Analogs. Acta Pol. Pharm. Drug Res. 2014, 71, 771–780. [Google Scholar]
  37. Mohamed, A.M.; Al-Qallawi, H.R.M.; El-Sayed, W.A.; Arafa, W.A.A.; Alhumaimus, M.S.; Hassan, A.K. Anticancer Activity of Newly Synthesized Triazolopyrimidine Derivatives and Their Nucleoside Analogs. Acta Pol. Pharm. Drug Res. 2015, 72, 307–318. [Google Scholar]
  38. El-Sayed, W.A.; Fathi, N.M.; Gad, W.A.; El-Ashry, E.S.H. Synthesis, and Antiviral Evaluation of Some 5-N-Arylaminomethyl-2-glycosylsulphanyl-1,3,4-oxadiazoles and their analogues against Hepatitis A and Herpes simplex viruses. J. Carbohydr. Chem. 2008, 27, 357–372. [Google Scholar] [CrossRef]
  39. Ibrahim, Y.A.; Abbas, A.A.; Elwahy, A.H.M. Selective synthesis and structure of 2-N- and 3-S-glucosyl-1,2,4-triazoles of potential biological interest. Carbohydr. Lett. 1999, 3, 331–338. [Google Scholar]
  40. Mansour, A.K.; Ibrahim, Y.A.; Khalil, N.S.A.M. Selective synthesis and structure of 6-arylvinyl-2- and 4-glucosyl-1,2,4-triazines of expected interesting biological activity. Nucleosides Nucleotides Nucleic Acids 1999, 18, 2256–2283. [Google Scholar] [CrossRef]
  41. Salih, N.A. Synthesis and Characterization of Novel Azole Heterocycles Based on 2,5-Disubstituted Thiadiazole. Turk. J. Chem. 2008, 32, 229–235. [Google Scholar]
  42. Yusuf, M.; Khan, R.A.; Ahmed, B. Syntheses and anti-depressant activity of 5-amino-1,3,4-thiadiazole-2-thiol imines and thiobenzyl derivatives. Bioorg. Med. Chem. 2008, 16, 8029–8034. [Google Scholar] [CrossRef] [PubMed]
  43. Hu, Y.; Li, C.-Y.; Wang, X.-M.; Yang, Y.-H.; Zhu, H.-L. 1,3,4-Thiadiazole: Synthesis, Reactions, and Applications in Medicinal, Agricultural, and Materials Chemistry. Chem. Rev. 2014, 114, 5583–5584. [Google Scholar] [CrossRef] [PubMed]
  44. Hamdy, N.A.; Anwar, M.M.; Abu-Zied, K.M.; Awad, H.M. Synthesis, tumor inhibitory and antioxidant activity of new polyfunctionally 2-substituted 5,6,7,8-tetrahydronaphthalene derivatives containing pyridine, thioxopyridine and pyrazolopyridine moieties. Acta. Pol. Pharm. Drug Res. 2013, 70, 987–1001. [Google Scholar]
  45. Soliman, H.A.; Yousif, M.N.M.; Said, M.M.; Hassan, N.A.; Ali, M.M.; Awad, H.M.; Abdel-Megeid, F.M.E. Synthesis of novel 1,6-naphthyridines, pyrano[3,2-c]pyridines and pyrido[4,3-d]pyrimidines derived from 2,2,6,6-tetramethylpiperidin-4-one for in vitro anticancer and antioxidant evaluation. Der. Pharma. Chem. 2014, 6, 394–410. [Google Scholar]
  46. Awad, H.M.; Abd-Alla, H.I.; Mahmoud, K.H.; El-Toumy, S.A. In vitro anti-nitrosative, antioxidant, and cytotoxicity activities of plant flavonoids: A comparative study. Med. Chem. Res. 2014, 23, 3298–3307. [Google Scholar] [CrossRef]
  • Sample Availability: Samples of the compounds are available from the authors.
Molecules 22 00170 g001 550
Figure 1. Anticancer spiro-thiazolidine (A and B), thiazolopyrimidine (C) and 1,3,4-thiadiazole (D) compounds.
Figure 1. Anticancer spiro-thiazolidine (A and B), thiazolopyrimidine (C) and 1,3,4-thiadiazole (D) compounds.
Molecules 22 00170 g001
Molecules 22 00170 sch001 550
Scheme 1. Synthesis of spiro[cyclohexane-1,2′-thiazolo[4,5-d]pyrimidine] glycosides.
Scheme 1. Synthesis of spiro[cyclohexane-1,2′-thiazolo[4,5-d]pyrimidine] glycosides.
Molecules 22 00170 sch001
Molecules 22 00170 sch002 550
Scheme 2. Synthesis of 1,3,4-thiadiazole thioglycoside.
Scheme 2. Synthesis of 1,3,4-thiadiazole thioglycoside.
Molecules 22 00170 sch002
Molecules 22 00170 g002 550
Figure 2. Anticancer activity of compounds against human liver hepatocellular carcinoma (HepG-2), human prostate adenocarcinoma (PC-3) and human colorectal carcinoma (HCT-116) cell lines using a 3-[4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay at 100 ppm.
Figure 2. Anticancer activity of compounds against human liver hepatocellular carcinoma (HepG-2), human prostate adenocarcinoma (PC-3) and human colorectal carcinoma (HCT-116) cell lines using a 3-[4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay at 100 ppm.
Molecules 22 00170 g002
Table
Table 1. The anticancer IC50 values of compounds using a MTT assay against HepG-2, PC-3 and HCT116 cell lines.
Table 1. The anticancer IC50 values of compounds using a MTT assay against HepG-2, PC-3 and HCT116 cell lines.
CompoundHCT-116PC-3HepG-2
IC50 (nM) ± SD
1178.1 ± 5.3184.1 ± 6.3509.5 ± 9.2
3201.3 ± 3.8210.2 ± 4.7541.4 ± 7.2
7120.1 ± 4.1223.9 ± 6.4134.5 ± 4.9
995.8 ± 3.7281.8 ±3.6285.1 ± 3.9
12250.3 ± 5.7437.4 ± 5.3425.6 ± 8.6
13204.6 ± 3.8217.7 ± 8.1581.5 ± 9.3
1492.2 ± 1.8120.8 ± 3.9176.1 ± 2.9
15204.6 ± 3.5331.3 ± 9.2348.5 ± 7.4
17127.4 ± 5.6693.9 ± 8.9209.7 ± 10.1
18106.3 ± 2.9111.6 ± 3.8334.1 ± 9.9
19106.6 ± 4.29493.8 ± 20.9599.0 ± 11.5
Doxorubicin126.7 ± 8.9129.7 ± 2.4117.1 ± 5.8

Share and Cite

MDPI and ACS Style

Flefel, E.M.; El-Sayed, W.A.; Mohamed, A.M.; El-Sofany, W.I.; Awad, H.M. Synthesis and Anticancer Activity of New 1-Thia-4-azaspiro[4.5]decane, Their Derived Thiazolopyrimidine and 1,3,4-Thiadiazole Thioglycosides. Molecules 2017, 22, 170. https://doi.org/10.3390/molecules22010170

AMA Style

Flefel EM, El-Sayed WA, Mohamed AM, El-Sofany WI, Awad HM. Synthesis and Anticancer Activity of New 1-Thia-4-azaspiro[4.5]decane, Their Derived Thiazolopyrimidine and 1,3,4-Thiadiazole Thioglycosides. Molecules. 2017; 22(1):170. https://doi.org/10.3390/molecules22010170

Chicago/Turabian Style

Flefel, Eman M., Wael A. El-Sayed, Ashraf M. Mohamed, Walaa I. El-Sofany, and Hanem M. Awad. 2017. "Synthesis and Anticancer Activity of New 1-Thia-4-azaspiro[4.5]decane, Their Derived Thiazolopyrimidine and 1,3,4-Thiadiazole Thioglycosides" Molecules 22, no. 1: 170. https://doi.org/10.3390/molecules22010170

Article Metrics

Back to TopTop