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Molecules 2016, 21(6), 779;

Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour

Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao 999078, China
Department of Chemistry, Hong Kong Baptist University, Hong Kong 999077, China
These authors contributed equally to this work.
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 3 May 2016 / Revised: 3 June 2016 / Accepted: 7 June 2016 / Published: 15 June 2016
(This article belongs to the Special Issue Natural Polysaccharides)
Full-Text   |   PDF [3349 KB, uploaded 15 June 2016]   |  


A neutral α-glucan, named BP1, with a molecular mass of approximately 9.45 kDa, was isolated from Lobelia chinensis by hot-water extraction, a Q-Sepharose Fast Flow column and Superdex-75 column chromatography. Its chemical structure was characterized by monosaccharide analysis, methylation analysis and analysis of its FT-IR, high performance gel permeation chromatography (HPGPC) and 1D/2D-NMR spectra data. The backbone of BP1 consists of →6α-d-Glcp16,3α-d-Glcp1→(6α-d-Glcp1)x-6,3α-d-Glcp1-(6α-d-Glcp1)y→. The side chains were terminal α-d-Glcp1→ and α-d-Glcp1→ (6α-d-Glcp1)z→4α-d-Glcp13α-d-Glcp14α-d-Glcp1→ (x + y + z = 5), which are attached to the backbone at O-3 of 3,6α-d-Glcp1. The results of the effect of BP1 on mouse macrophage cell line RAW 264.7 indicate that BP1 enhances the cell proliferation, phagocytosis, nitric oxide production and cytokine secretion in a dose-dependent manner. Because the inhibitor of Toll-like receptor 4 blocks the BP1-induced secretion of TNF-α and IL-6, we hypothesize that α-glucan BP1 activates TLR4, which mediates the above-mentioned immunomodulating effects. View Full-Text
Keywords: Lobelia chinensis; NMR; RAW 264.7; immunomodulating; TLR4 Lobelia chinensis; NMR; RAW 264.7; immunomodulating; TLR4

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Li, X.-J.; Bao, W.-R.; Leung, C.-H.; Ma, D.-L.; Zhang, G.; Lu, A.-P.; Wang, S.-C.; Han, Q.-B. Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour. Molecules 2016, 21, 779.

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