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Correction published on 6 January 2020, see Molecules 2020, 25(1), 232.
Open AccessArticle

Dioscin Induces Apoptosis in Human Cervical Carcinoma HeLa and SiHa Cells through ROS-Mediated DNA Damage and the Mitochondrial Signaling Pathway

College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian 116044, China
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Academic Editor: Derek J. McPhee
Molecules 2016, 21(6), 730; https://doi.org/10.3390/molecules21060730
Received: 30 April 2016 / Revised: 24 May 2016 / Accepted: 31 May 2016 / Published: 4 June 2016
Dioscin, a natural product, has activity against glioblastoma multiforme, lung cancer and colon cancer. In this study, the effects of dioscin against human cervical carcinoma HeLa and SiHa cells were further confirmed, and the possible mechanism(s) were investigated. A transmission electron microscopy (TEM) assay and DAPI staining were used to detect the cellular morphology. Flow cytometry was used to assay cell apoptosis, ROS and Ca2+ levels. Single cell gel electrophoresis and immunofluorescence assays were used to test DNA damage and cytochrome C release. The results showed that dioscin significantly inhibited cell proliferation and caused DNA damage in HeLa and SiHa cells. The mechanistic investigation showed that dioscin caused the release of cytochrome C from mitochondria into the cytosol. In addition, dioscin significantly up-regulated the protein levels of Bak, Bax, Bid, p53, caspase-3, caspase-9, and down-regulated the protein levels of Bcl-2 and Bcl-xl. Our work thus demonstrated that dioscin notably induces apoptosis in HeLa and SiHa cells through adjusting ROS-mediated DNA damage and the mitochondrial signaling pathway. View Full-Text
Keywords: apoptosis; dioscin; DNA damage; ROS; cervical carcinoma apoptosis; dioscin; DNA damage; ROS; cervical carcinoma
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MDPI and ACS Style

Zhao, X.; Tao, X.; Xu, L.; Yin, L.; Qi, Y.; Xu, Y.; Han, X.; Peng, J. Dioscin Induces Apoptosis in Human Cervical Carcinoma HeLa and SiHa Cells through ROS-Mediated DNA Damage and the Mitochondrial Signaling Pathway. Molecules 2016, 21, 730.

AMA Style

Zhao X, Tao X, Xu L, Yin L, Qi Y, Xu Y, Han X, Peng J. Dioscin Induces Apoptosis in Human Cervical Carcinoma HeLa and SiHa Cells through ROS-Mediated DNA Damage and the Mitochondrial Signaling Pathway. Molecules. 2016; 21(6):730.

Chicago/Turabian Style

Zhao, Xinwei; Tao, Xufeng; Xu, Lina; Yin, Lianhong; Qi, Yan; Xu, Youwei; Han, Xu; Peng, Jinyong. 2016. "Dioscin Induces Apoptosis in Human Cervical Carcinoma HeLa and SiHa Cells through ROS-Mediated DNA Damage and the Mitochondrial Signaling Pathway" Molecules 21, no. 6: 730.

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Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

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