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Molecules 2014, 19(9), 14417-14445;

Enzyme Molecules in Solitary Confinement

Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Regensburg 93040, Germany
Author to whom correspondence should be addressed.
Received: 4 August 2014 / Revised: 3 September 2014 / Accepted: 3 September 2014 / Published: 12 September 2014
(This article belongs to the Special Issue Single Molecule Techniques)
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Large arrays of homogeneous microwells each defining a femtoliter volume are a versatile platform for monitoring the substrate turnover of many individual enzyme molecules in parallel. The high degree of parallelization enables the analysis of a statistically representative enzyme population. Enclosing individual enzyme molecules in microwells does not require any surface immobilization step and enables the kinetic investigation of enzymes free in solution. This review describes various microwell array formats and explores their applications for the detection and investigation of single enzyme molecules. The development of new fabrication techniques and sensitive detection methods drives the field of single molecule enzymology. Here, we introduce recent progress in single enzyme molecule analysis in microwell arrays and discuss the challenges and opportunities. View Full-Text
Keywords: enzyme kinetics; single molecule enzymology; fluorescence microscopy; femtoliter array enzyme kinetics; single molecule enzymology; fluorescence microscopy; femtoliter array

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Liebherr, R.B.; Gorris, H.H. Enzyme Molecules in Solitary Confinement. Molecules 2014, 19, 14417-14445.

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