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Molecules 2014, 19(9), 14128-14138;

Simultaneous Quantification of Three Polymorphic Forms of Carbamazepine in the Presence of Excipients Using Raman Spectroscopy

Department of Chemistry, Federal University of São Carlos, Rodovia Washington Luiz Km 235, São Carlos, SP 13565-905, Brazil
Author to whom correspondence should be addressed.
Received: 9 July 2014 / Revised: 29 August 2014 / Accepted: 1 September 2014 / Published: 9 September 2014
(This article belongs to the Special Issue Advances of Vibrational Spectroscopic Technologies in Life Sciences)
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The occurrence of polymorphic transitions is a serious problem for pharmaceutical companies, because it can affect the bioavailability of the final product. With several known polymorphic forms carbamazepine is one of the most problematic drugs in this respect. Raman spectroscopy is a vibrational technique that is becoming very important in the pharmaceutical field, mainly due to its highly specific molecular fingerprint capabilities and easy use as a process analytical tool. However, multivariate methods are necessary both for identification and quantification. In this work an analytical methodology using Raman spectroscopy and interval Partial Least Squares Regression (iPLS), was developed in order to quantify mixtures of carbamazepine polymorphs in the presence of the most common excipients. The three polymorphs CBZ I, CBZ III and CBZ DH (which is a dihydrate) were synthesized and characterized by PXRD and DSC. Subsequently, tablets were manufactured using excipients and 15 different mixtures of carbamazepine polymorphs. The iPLS model presented average prediction validation errors of 1.58%, 1.04% and 0.22% wt/wt, for CBZ I, CBZ III and CBZ DH, respectively, considering the whole mass of the tablet. The model presents a good prediction capacity and the proposed methodology could be used to perform quality control in final products. View Full-Text
Keywords: carbamazepine; polymorphism; Raman spectroscopy; iPLS; multivariate calibration carbamazepine; polymorphism; Raman spectroscopy; iPLS; multivariate calibration

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Farias, M.; Carneiro, R. Simultaneous Quantification of Three Polymorphic Forms of Carbamazepine in the Presence of Excipients Using Raman Spectroscopy. Molecules 2014, 19, 14128-14138.

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