Next Article in Journal
Photobleaching Kinetics and Time-Integrated Emission of Fluorescent Probes in Cellular Membranes
Next Article in Special Issue
Synthesis and in Vitro Antimicrobial Evaluation of New N-Heterocyclic Diquaternary Pyridinium Compounds
Previous Article in Journal
Bifunctionalized Allenes. Part XV. Synthesis of 2,5-dihydro-1,2-oxaphospholes by Electrophilic Cyclization Reaction of Phosphorylated α-Hydroxyallenes
Previous Article in Special Issue
Synthesis and Biological Evaluation of 2,4-Diaminopyrimidine-Based Antifolate Drugs against Bacillus anthracis
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 19
Issue 8
10.3390/molecules190811077
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Activity of Antifungal Organobismuth(III) Compounds Derived from Alkyl Aryl Ketones against S. cerevisiae: Comparison with a Heterocyclic Bismuth Scaffold Consisting of a Diphenyl Sulfone

by
Toshihiro Murafuji
1,*,
Mai Tomura
1,
Katsuya Ishiguro
2 and
Isamu Miyakawa
2
1
Graduate School of Medicine, Yamaguchi University, Yamaguchi 753-8512, Japan
2
Graduate School of Science and Engineering, Yamaguchi University, Yamaguchi 753-8512, Japan
*
Author to whom correspondence should be addressed.
Molecules 2014, 19(8), 11077-11095; https://doi.org/10.3390/molecules190811077
Submission received: 27 May 2014 / Revised: 19 July 2014 / Accepted: 21 July 2014 / Published: 29 July 2014
(This article belongs to the Special Issue Heterocyclic and Medicinal Chemistry)
Browse Figures
Versions Notes

Abstract

:
A series of hypervalent organobismuth(III) compounds derived from alkyl aryl ketones [XBi(5-R'C6H3-2-COR)(Ar)] was synthesized to investigate the effect of the compounds’ structural features on their antifungal activity against the yeast Saccharomyces cerevisiae. In contrast to bismuth heterocycles [XBi(5-RC6H3-2-SO2C6H4-1'-)] derived from diphenyl sulfones, a systematic quantitative structure-activity relationship study was possible. The activity depended on the Ar group and increased for heavier X atoms, whereas lengthening the alkyl chain (R) or introducing a substituent (R') reduced the activity. IBi(C6H4-2-COCH3)(4-FC6H4) was the most active. Its activity was superior to that of the related acyclic analogues ClBi[C6H4-2-CH2N(CH3)2](Ar) and ClBi(C6H4-2-SO2tert-Bu)(Ar) and also comparable to that of heterocyclic ClBi(C6H4-2-SO2C6H4-1'-), which was the most active compound in our previous studies. Density function theory calculations suggested that hypervalent bismuthanes undergo nucleophilic addition with a biomolecule at the bismuth atom to give an intermediate ate complex. For higher antifungal activity, adjusting the lipophilicity-hydrophilicity balance, modeling the three-dimensional molecular structure around the bismuth atom, and stabilizing the ate complex appear to be more important than tuning the Lewis acidity at the bismuth atom.

Graphical Abstract

1. Introduction

Biologically active bismuth compounds are the subject of considerable interest, although there have been only a few biological studies of organobismuth compounds [1,2,3,4,5,6]. This is in contrast to their growing use in synthetic reactions [7,8,9]. We recently reported the antifungal activity of hypervalent organobismuth(III) and (V) compounds against the yeast Saccharomyces cerevisiae [10,11,12]. The Lewis acidity at the bismuth center was essential for the activity. Compounds A–D are hypervalent organobismuth(III) derivatives, which are generally expressed by a “10-Bi-4” notation based on the number of total electrons in the outermost shell and bonds to the relevant heteroatom center [13,14] (Figure 1). Compounds A and B showed moderate activity but C1 was much more active [10] (Table 1). The higher activity of C1 can be attributed to its structural characteristics as well as the biopharmaceutical properties. Its lower ClogP value (1.18) indicates lower lipophilicity, the lack of bulky alkyl substituents on the sulfonyl group reduces steric crowding around the tetracoordinate bismuth atom, and weaker intramolecular coordination of the sulfonyl substituent arises from the longer intramolecular Bi–O atomic distance [2.775(6) Å] [10] because of the rigid heterocyclic structure and the highly coordinated geometry of the bismuth atom.
Molecules 19 11077 g001 550
Figure 1. Molecular structure of hypervalent organobismuth compounds.
Figure 1. Molecular structure of hypervalent organobismuth compounds.
Molecules 19 11077 g001
Table
Table 1. Antifungal assay for bismuthanes.
Table 1. Antifungal assay for bismuthanes.
CompoundInhibition Zone (mm)ClogPCompoundInhibition Zone (mm)ClogP
A1133.526a82.54
A2123.026b123.12
B183.286c162.63
B2122.786d172.77
C1181.186e153.34
C280.816f04.12
D1173.497a92.54
D2124.667b153.12
7c172.63
4b05.817d192.77
4c04.827e143.34
4d05.107f04.12
4e06.249a103.62
5a82.549b74.55
5b133.129c103.34
5c152.6311a94.18
5d162.7711b84.71
5e143.3411c103.96
5f04.12Nystatin30
In particular, the antifungal activity of monosubstituted C (R2 = H) decreases as ClogP increases, which in turn depends on substituent R1 [11]. On the basis of this relationship, we synthesized C2, which has hydrophilic substituents, but its activity was less than that of parent C1 [11]. Yet, compared with C1, bismuth carboxylates D showed moderate to high activity despite their much higher lipophilicity. To explain this, we proposed that in the yeast cell the bismuth-carboxylate or bismuth-halogen bonds are cleaved into a cationic bismuth scaffold and a carboxylate or halide anion, and that the bismuth scaffold plays an important role in the inhibition activity [12]. Stable cationic organobismuth complexes have been isolated and characterized [15,16]. These findings indicate that antifungal activity is not just affected by the hydrophilicity or lipophilicity.
To design bismuthanes that are more active than C1, it is important to understand how their structural features affect their antifungal activity. Toward this end, we studied a series of halobismuthanes (5–7, 9 and 11) derived from alkyl aryl ketone (Scheme 1, Scheme 2 and Scheme 3). Unlike the diphenyl sulfone structure of the heterocyclic bismuth scaffold C, the structures in this series could be systematically modified by changing the alkyl and aryl groups of the ketone scaffold as well as the aryl and halo groups attached to the bismuth atom. Furthermore, the various biological activities of acetophenone derivatives were attractive from the viewpoint of bioavailability [17,18,19]. Through this study, we aimed to better elucidate the effect of structural characteristics on the antifungal activity and also to design a halobismuthane (7d) that was comparable to C1 in activity.
Molecules 19 11077 g005 550
Scheme 1. Synthesis of silyl enol ethers.
Scheme 1. Synthesis of silyl enol ethers.
Molecules 19 11077 g005
Molecules 19 11077 g006 550
Scheme 2. Synthesis of bismuthanes 4 and their transformation.
Scheme 2. Synthesis of bismuthanes 4 and their transformation.
Molecules 19 11077 g006
Molecules 19 11077 g007 550
Scheme 3. Synthesis of bismuthanes 8 and 10 and their transformation.
Scheme 3. Synthesis of bismuthanes 8 and 10 and their transformation.
Molecules 19 11077 g007

2. Results and Discussion

2.1. Synthesis

Silyl enol ethers 1–3 were prepared from the parent ketones [20,21] and used as starting materials (Scheme 1). We have previously reported the synthesis of 4a–c and 4e, their corresponding bromides 6, and 5b and 7b, but the yield of parent compound 4 is very low [22] because the lithiated silyl enol ethers [23] and chlorobismuthanes are reactive. Hence, 4 and its homologues 8 and 10 were synthesized by modifying our previous method (Scheme 2 and Scheme 3). The reactive lithiated silyl enol ethers and chlorobismuthanes (Ar2BiCl) were replaced by the less reactive magnesiated derivatives and iodobismuthanes (Ar2BiI), respectively. This made the synthesis of triarylbismuthanes 4, 8, and 10 more reliable. The triarylbismuthanes were converted into the corresponding halobismuthanes 5–7, 9, and 11 by treatment with boron trifluoride diethyl ether complex and subsequent halogenation. Because 4a, 4f, and 10 decomposed during purification by column chromatography, they were isolated as the corresponding halobismuthanes. Compound 10c was isolated as bromobismuthane 11c because the corresponding chlorobismuthane was unstable. In the 1H-NMR spectra of the halobismuthanes, the ortho proton adjacent to the bismuth atom in the aryl ketone scaffold underwent anisotropic deshielding because of its close proximity to the electronegative chlorine, bromine or iodine atom attached to the bismuth center [24]. The ortho proton signal for 5b, 6b, and 7b appeared at δ 9.06, 9.21 and 9.42 ppm, respectively. Furthermore, compared with parent bismuthane 4b, the carbonyl carbon signal in the 13C-NMR spectra of 5b, 6b, and 7b was shifted downfield by 6–8 ppm. The carbonyl stretching vibration in the IR spectrum of the halobismuthanes also showed a shift to a lower frequency by 40 cm−1. These observations indicate that the carbonyl oxygen atom was coordinated to the bismuth atom to form a hypervalent C=O•••Bi–Cl, C=O•••Bi–Br, or C=O•••Bi–I bonds. Hypervalent bond formation in 6b has been confirmed by X-ray crystallography [22]. Halobismuthanes 5–7, 9, and 11 were stable in water and DMSO, indicating that the hypervalent bond formation suppresses hydrolysis and redistribution reactions of these compounds.

2.2. Growth Inhibition Tests against S. cerevisiae

Initially, we tested the inhibition activity of 4–7 ( Experimental Subsection 3.6, Table 1, and Figure 2). Triarylbismuthanes 4b–e were inactive, but halobismuthanes 5–7 showed moderate to high antifungal activity. This is consistent with our previous finding that the Lewis acidic bismuth center is the active site [10,11,12]. Iodobismuthanes 7 showed higher activity than bromobismuthanes 6, which were slightly more active than chlorobismuthanes 5. This suggests that the activity increases with decreasing the electronegativity of the halo substituent. It should be noted that halobismuthanes 5d, 6d, and 7d bearing a p-fluorophenyl group showed the highest activity among each compound’s halogen homologues a–f. Although 5a, 6a, and 7a bearing a p-methoxyphenyl group were the most hydrophilic among each compound’s homologues, they were not very active because their solubility was reduced by the aryl group [25]. Halobismuthanes 5f, 6f, and 7f bearing a mesityl group were inactive. Next, we determined the inhibition activity of 9a–c, which were prepared from p-substituted acetophenones. The activity of 9a–c was reduced by the p-substituent. This trend is similar to that observed in the quantitative structure-activity relationship study of C. In view of the higher activity of halobismuthanes 5d, 6d, and 7d, an attempt to synthesize 9 bearing a p-fluoro substituent in the acetophenone scaffold failed: It was difficult to control the lithiation of the corresponding silyl enol ether because the electronegativity of the fluoro substituent increased the reactivity of the ether toward the lithiation. We replaced the methyl substituent of the acetyl group in 5b with various alkyl substituents to obtain 11a–c, but they were less active. Halobismuthane 7d showed the highest activity of the compounds in this study, and its activity was also comparable to that of C1. Although the activity of 7d was not as high as the standard antifungal drug, nystatin, this finding demonstrates the high antifungal activity of organobismuth compounds. A certain organobismuth compound has been reported to exhibit selective cytotoxicity to vascular endothelial cells, which may be applicable in antivascular cancer therapy [26]. However, it remains to be determined whether 7d and its related compounds exhibit specific cytotoxicity against fungi.
Molecules 19 11077 g002 550
Figure 2. Plot of the antifungal assay for bismuthanes 5–7 and 9.
Figure 2. Plot of the antifungal assay for bismuthanes 5–7 and 9.
Molecules 19 11077 g002

2.3. Structure-Activity Relationship

Based on this study, the structure-activity relationship of the hypervalent bismuthanes may be summarized as follows. Firstly, steric crowding around the tetracoordinate bismuth atom substantially lowers antifungal activity. The lack of activity of 5f, 6f, and 7f was attributed to the bulky mesityl group, which possibly blocks the interaction of the bismuth atom with the biomolecules in the yeast. The long chain or branched RCO group in 11a–c may also play a similar role, as suggested by A and B bearing Me2N and tert-BuSO2 groups, respectively. Secondly, the hydrophilicity of the hypervalent bismuthanes is not always proportional to their antifungal activity, as was observed in 5a, 6a, and 7a, and C2. In particular, 7d is less hydrophilic (ClogP = 2.77) than C1 (ClogP = 1.18) but comparably more active. This suggests that the relationship in homologous series C between activity and ClogP does not apply to 7d, which contains acetophenone, and is completely different from diphenyl sulfone in terms of structure and properties. Thirdly, the heterocyclic structure in C1 is not essential for high antifungal activity. Fourthly, introducing a fluoro substituent at an appropriate position in the hypervalent bismuthanes enhances the antifungal activity. The enhancement of biological activity by fluoro substituents is well known in drugs and pesticides [27,28,29]. Fifthly, the Lewis acidity at the bismuth atom, although essential, does not correspond directly to the antifungal activity. Thus, 5a, 5f, 6b, and 7d showed a wide variation in activity depending on the aryl and halo groups (Table 1), although the Lewis acidity of the bismuth atoms of these compounds were similar as assessed by the carbonyl carbon signals (δ ppm) and the carbonyl stretching bands (ν cm−1) in the 13C-NMR and IR spectra, respectively (5a, 209.2, 1620; 5f, 208.1, 1630; 6b, 208.7, 1625; and 7d, 207.7, 1630). Therefore, we compared the Lewis acidity at the tetracoordinate bismuth atom between different scaffolds by using the intramolecular Bi–O and Bi–N distances and examined their relationship to the antifungal activity. The length of the metal-ligand bond has been used to quantify the Lewis acidity at the metal center [30,31]. We have previously determined the Bi–O or Bi–N intramolecular distance in 6b [22], A1 [32], B1 [24], C1 [10], C2 [11], and D2 [12] by X-ray crystallography. We expected that the metal-ligand interactions would decrease as the intramolecular distance is shortened because the metal-ligand interactions would reduce the Lewis acidity at the metal center [33]. Hence, the Lewis acidity in these compounds should be in the order C1 [2.775(6) Å] > D2 [2.754(2) Å] > C2 [2.634(7) Å] > B1 [2.592(5) Å] > A1 [2.525(6) Å] > 6b [2.519(7) Å]. However, the antifungal activity decreased in the order C1 > A1 > D2, 6b > B1, C2, which did not correspond to the order of the intramolecular distance. Although hypervalent bond formation is known to enhance the Lewis acidity at the metal center [34,35,36,37,38], the enhancement of the Lewis acidity at the bismuth atom in these bismuthanes, even if it does occur, appears to be very slight because only one electron-accepting Bi–X bond is present.

2.4. Plausible Mechanism of Action for Hypervalent Bismuthanes

We have proposed that C and D undergo cleavage of the Bi–Cl or Bi–O bond in the yeast cell to form a cationic bismuth scaffold, which plays an important role in the inhibition activity [12]. A plausible pathway for this reaction is shown as Path 1 in Scheme 4. Although we have not identified the biomolecules to which these bismuthanes bind, we expect that the Lewis acidic bismuth atom should have a high affinity for thiol groups [39]. Finally, we examined how the dissociation energy of the bismuth-halogen or bismuth-oxygen bond in Path 1 affects the antifungal activity. Using density functional theory [40], we calculated the dissociation energy (enthalpies) of these bonds in A–D, 5–7, and 9 (Table 2, Path 1).
Molecules 19 11077 g008 550
Scheme 4. Plausible reaction pathway of the bismuthane.
Scheme 4. Plausible reaction pathway of the bismuthane.
Molecules 19 11077 g008
Table
Table 2. Dissociation and association energy.
Table 2. Dissociation and association energy.
Ar2BiXInhibition Zone (mm)Path 1 (kcal/mol)Path 2 (kcal/mol)Ar2BiXInhibition Zone (mm)Path 1 (kcal/mol)Path 2 (kcal/mol)
A11312.82–8.186a817.93–12.29
A21213.12–8.466b1218.38–12.33
B1819.33–13.516c1618.73–12.65
B21219.66–13.856d1719.06–13.29
C11825.22–17.226e1519.37–13.38
C2824.75–17.156f018.15–
D11736.93–10.097a915.81–15.18
D21235.49–10.977b1516.22–15.13
5a816.93–12.087c1716.59–15.43
5b1317.37–12.007d1916.89–16.00
5c1517.75–12.337e1417.14–16.26
5d1618.07–13.027f018.15-
5e1418.36–13.149a1016.83–11.71
5f018.15-9c1016.54–11.32
Contrary to our expectation, very active C1 and D1 had highly endothermic dissociation energy (25.22 and 36.93 kcal/mol, respectively), indicating that generating the bismuth cation requires high energy. Furthermore, the dissociation energy of moderately active A1 was quite low owing to the stabilization of the bismuth cation by a strong coordination with the dimethylamino group. However, the most active compound, 7d, was not as endothermic (16.89 kcal/mol), which is consistent with our proposed mechanism. Comparing the dissociation energy of 7a, 7c, and 7d, and of 5a, 5c, and 5d, revealed that the p-fluorophenyl group in 5d and 7d destabilizes the bismuth cation. No correlation was observed between dissociation energy and antifungal activity (Figure 3). Hence, we concluded that the bismuth cations are not generated from hypervalent bismuthanes in yeast cells. Alternatively, we propose that an intermediate ate complex [Ar2BiXNu]− is formed by the bismuthanes undergoing addition at the bismuth atom with biomolecules such as hydrolases (Scheme 4, Path 2). After the release of X−, the resulting Ar2BiNu species is delivered to the organelle responsible for the antifungal activity via a further biological process. We calculated the association energy (enthalpies) by using methanethiolate anion as a model nucleophile (Nu−) (Table 2, Path 2). Among all the bismuthanes, C1 and 7d had some of the most exothermic association reaction, indicating that this path is preferable. Unlike Path 1, a weak but positive correlation between association energy and antifungal activity was observed (Figure 4). It should be stressed that in 5f, 6f, and 7f, which contained a mesityl group, no minimum energy path connects the reactants and the product. This is in good agreement with their lack of activity. The activity of 7 was higher than that of 5 and 6, although the activity of C1 and its bromo and iodo analogues did not depend on the halo group [10]. This may be because the intramolecular Bi–O interaction of the carbonyl group is stronger than that of the sulfonyl group, which slightly enhances the Lewis acidity [34,35,36,37,38] at the bismuth atom and promotes the nucleophilic addition of Nu−. Such enhancement of the Lewis acidity has been observed in triorganotin halides bearing an intramolecular coordination with a carbonyl group [41].
Molecules 19 11077 g003 550
Figure 3. Plot of the dissociation energy against the antifungal activity.
Figure 3. Plot of the dissociation energy against the antifungal activity.
Molecules 19 11077 g003
Molecules 19 11077 g004 550
Figure 4. Plot of the association energy against the antifungal activity.
Figure 4. Plot of the association energy against the antifungal activity.
Molecules 19 11077 g004

3. Experimental Section

3.1. General Information

All reactions were carried out under argon unless otherwise noted. Hexane, diethyl ether and dichloromethane were distilled from calcium hydride before use. Melting points were determined on a Yanagimoto melting point apparatus without correction. 1H- and 13C-NMR spectra were recorded in CDCl3 or DMSO-d6 on a Bruker Avance 400S spectrometer with TMS as an internal standard. IR spectra were obtained as KBr pellets on a Nicolet FT-IR Impact 410 spectrophotometer. Elemental analyses were performed on a Micro Corder JM10 apparatus (J-Science Lab. Co., Kyoto, Japan).

3.2. Synthesis of 4, 8 and 10

A typical example is exemplified by the synthesis of 4b: To a stirred solution of TMEDA (1.36 mL, 9 mmol) in hexane (5 mL) was added dropwise at ice bath temperature n-butyllithium (9 mmol) in hexane followed by acetophenone trimethylsilyl enol ether 1 (576 mg, 3 mmol), and the mixture was stirred for 24 h at room temperature. In a separate flask, a mixture of bismuth(III) chloride (315.5 mg, 1 mmol) and tris(4-methylphenyl)bismuthane (964 mg, 2 mmol) was stirred in ether (10 mL) at room temperature for 1 h. To the suspension of chlorobis(4-methylphenyl)bismuthane (ca. 3 mmol) thus formed was added sodium iodide (450 mg, 3 mmol) and a few drops of 15-crown-5 ether and the resulting yellowish mixture was stirred for 3 h at room temperature. To a suspension of the lithium compound previously prepared was added at room temperature magnesium dibromide diethyl etherate (775 mg, 3 mmol) followed by, at −30 °C, a suspension of iodobis(4-methylphenyl)bismuthane (ca. 9 mmol), and the resulting mixture was stirred for 1 h, during which time the temperature was raised to ambient temperature. The reaction mixture was poured into brine (50 mL) and extracted with ethyl acetate (50 mL × 3). The combined extracts were concentrated to leave an oily residue, which was purified by chromatography (silica gel) using hexane-ethyl acetate (5:1) as the eluent to afford 4b in 30% yield (459 mg, 0.9 mmol). Because 4a, 4f and 10 underwent decomposition when purified by chromatography on silica gel, these compounds were converted into the corresponding halobismuthanes 5a, 5f and 11, respectively, without isolation.
(2-Acetylphenyl)bis(4-methylphenyl)bismuthane (4b). Yield: 30%; mp 114–116 °C; 1H-NMR (400 MHz, CDCl3): δ 2.30 (6H, s), 2.62 (3H, s), 7.17 (4H, d, J = 7.8 Hz), 7.41 (1H, dt, J = 1.4, 7.6 Hz), 7.47 (1H, dt, J = 1.4, 7.6 Hz), 7.57 (4H, d, J = 7.8 Hz), 7.93 (1H, dd, J = 1.4, 7.6 Hz), 8.14 (1H, dd, J = 1.4, 7.6 Hz); 13C-NMR (100 MHz, CDCl3): δ 21.5, 27.2, 127.5, 131.2, 132.2, 135.0, 136.8, 137.7, 140.3, 141.5, 158.5, 159.8, 201.1. IR (KBr): ν = 1665, 1260, 790, 765, 600 and 480 cm−1. Anal. Calc. for C22H21BiO: C, 51.77; H, 4.15. Found: C, 51.70; H, 4.00%.
(2-Acetylphenyl)diphenylbismuthane (4c). Yield: 16%; mp 96–98 °C; 1H-NMR (400 MHz, CDCl3): δ 2.63 (3H, s), 7.29 (2H, t, J = 7.6 Hz), 7.36 (4H, t, J = 7.6 Hz), 7.42 (1H, dt, J = 1.2, 7.6 Hz), 7.49 (1H, dt, J = 1.4, 7.6 Hz), 7.69 (4H, d, J = 7.6 Hz), 7.91 (1H, dd, J = 1.2, 7.6 Hz), 8.16 (1H, dd, J = 1.2, 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.2, 127.3, 127.6, 130.3, 132.3, 135.2, 137.7, 140.4, 141.5, 201.2; ipso carbon signals were too weak to be observed. IR (KBr): ν = 1660, 1260, 1090, 1000, 800, 760, 725, 700 and 600 cm−1. Anal. Calc. for C20H17BiO: C, 49.80; H, 3.55. Found: C, 49.60; H, 3.50%.
(2-Acetylphenyl)bis(4-fluorophenyl)bismuthane (4d). Yield: 11%; mp 147–149 °C; 1H-NMR (400 MHz, CDCl3): δ 2.64 (3H, s), 7.00–7.05 (4H, m), 7.46 (1H, dt, J = 1.6, 7.6 Hz), 7.52 (1H, dt, J = 1.6, 7.6 Hz), 7.58–7.62 (4H, m), 7.84 (1H, dd, J = 1.2, 7.2 Hz), 8.18 (1H, dd, J = 1.2, 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.2, 117.7 (d, JCF = 19 Hz), 127.9, 132.5, 135.3, 139.3 (d, JCF = 6.0 Hz), 140.1, 141.3, 156.8 (br), 160.3 (br), 162.5 (d, JCF = 245 Hz), 201.4. IR (KBr): ν = 1660, 1570, 1480, 1210, 1160, 820, 770, 600 and 500 cm−1. Anal. Calc. for C20H15BiF2O: C, 46.35; H, 2.92. Found: C, 46.37; H, 2.88%.
(2-Acetylphenyl)bis(4-chlorophenyl)bismuthane (4e). Yield: 23%; mp 129–131 °C; 1H-NMR (400 MHz, CDCl3): δ 2.64 (3H, s), 7.30 (4H, d, J = 8.0 Hz), 7.46 (1H, dt, J = 1.2, 7.6 Hz), 7.52 (1H, dt, J = 1.2, 7.5 Hz), 7.57 (4H, d, J = 8.0 Hz), 7.84 (1H, dd, J = 1.2, 7.2 Hz), 8.19 (1H, dd, J = 1.2, 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.2, 128.0, 130.6, 132.6, 133.7, 135.5, 139.0, 140.2, 141.3, 201.4; ipso carbon signals were too weak to be observed. IR (KBr): ν = 1660, 1260, 1090, 1000, 800, 765 and 710 cm−1. Anal. Calc. for C20H15BiCl2O: C, 43.58; H, 2.74. Found: C, 43.40; H, 2.70%.
(2-Acetyl-4-methylphenyl)bis(4-methylphenyl)bismuthane (8a). Yield: 18%; mp 148–150 °C; 1H-NMR (400 MHz, CDCl3): δ 2.22 (3H, s), 2.30 (6H, s), 2.59 (3H, s), 7.16 (4H, d, J = 7.6 Hz), 7.26 (1H, d, J = 7.6 Hz), 7.57 (4H, d, J = 7.6 Hz), 7.74 (1H, d, J = 1.3 Hz), 8.03 (1H, d, J = 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 21.5, 21.6, 27.1, 128.2, 131.1, 132.2, 136.7, 137.7, 139.2, 141.03, 145.54, 158.48, 159.96, 200.6. IR (KBr): ν = 1660, 1580, 1290, 830, 790 and 480 cm−1. Anal. Calc. for C23H23BiO: C, 52.68; H, 4.42. Found: C, 52.32; H, 4.38%.
(2-Acetyl-4-isopropylphenyl)bis(4-methylphenyl)bismuthane (8b). Yield: 22%; mp 145–147 °C; 1H-NMR (400 MHz, CDCl3): δ 1.07 (6H, d, J = 7.2 Hz), 2.30 (6H, s), 2.60 (3H, s), 2.77 (1H, sep, J = 7.2 Hz), 7.16 (4H, d, J = 7.6 Hz), 7.30 (1H, dd, J = 1.6, 8.0 Hz), 7.58 (4H, d, J = 7.6 Hz), 7.78 (1H, d, J = 1.6 Hz), 8.06 (1H, d, J = 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ21.5, 23.4, 27.1, 34.0, 125.5, 131.0, 132.3, 136.7, 137.7, 139.5, 155.7, 158.6, 160.0, 200.5. IR (KBr): ν = 1650, 1580, 1350, 1190, 1050, 830, 790, 590 and 480 cm−1. Anal. Calc. for C25H27BiO: C, 54.35; H, 4.93. Found: C, 54.28; H, 4.84%.
(2-Acetyl-4-methoxyphenyl)bis(4-methylphenyl)bismuthane (8c). Yield: 8%; mp 135–137 °C; 1H-NMR (400 MHz, CDCl3): δ2.27 (3H, s), 2.32 (6H, s), 3.84 (3H, s), 7.02 (1H, d, J = 8.8 Hz), 7.20 (4H, d, J = 7.6 Hz), 7.58 (4H, d, J = 7.6 Hz), 7.99 (1H, dd, J = 2.0, 8.4 Hz), 8.15 (1H, d, J = 2.0 Hz). 13C-NMR (100 MHz, CDCl3): δ 21.4, 26.2, 55.8, 109.4, 130.6, 131.2, 133.1, 137.3, 137.5, 139.7, 143.0, 151.1, 165.6, 197.2. IR (KBr): ν = 1670, 1580, 1240, 1010, 800, 580 and 480 cm−1. Anal. Calc. for C23H23BiO2: C, 51.12; H, 4.29. Found: C, 50.88; H, 4.28%.

3.3. Synthesis of 5a, 5f and 11

A typical example is exemplified by the synthesis of 5a: An oily residue containing 4a obtained by the concentration of the extracted organic layer was dissolved in dichloromethane (5 mL) and boron trifluoride etherate (3 mmol) was added to the solution at 0 °C until 4a was completely consumed (checked by TLC). The mixture was diluted by the addition of brine (5 mL) and the organic layer was extracted with ethyl acetate (20 mL × 3). The combined extracts were concentrated to leave an oily residue, which was crystallized from MeOH to give 5a in 18% yield.
(2-Acetylphenyl)chloro(4-methoxylphenyl)bismuthane (5a). Yield: 18%; mp 156–158 °C; 1H-NMR (400 MHz, CDCl3): δ 2.69 (3H, s), 3.73 (3H, s), 7.01 (2H, d, J = 8.8 Hz), 7.63 (1H, dt, J = 1.2, 8.8 Hz), 8.00 (1H, dt, J = 1.2, 8.8 Hz), 8.03 (2H, d, J = 8.8 Hz), 8.25 (1H, d, J = 8.0 Hz), 9.06 (1H, d, J = 6.8 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.4, 55.0, 117.4, 128.3, 135.2, 138.0, 138.1, 138.3, 143.1, 159.5, 172.8, 185.3, 209.2. IR (KBr): ν = 1620, 1580, 1490, 1280, 1250, 810, 770 and 620 cm−1. Anal. Calc. for C15H14BiClO: C, 39.62; H, 3.10. Found: C, 39.42; H, 2.94%.
(2-Acetylphenyl)chloro(2,4,6-trimethylphenyl)bismuthane (5f). Yield: 13%; mp 172–174 °C; 1H-NMR (400 MHz, CDCl3): δ2.18 (3H, s), 2.46 (6H, s), 2.63 (3H, s), 7.12 (2H, s) 7.61 (1H, dt, J = 1.2, 7.6 Hz), 7.95 (1H, dt, J = 1.2, 7.6 Hz), 8.22 (1H, dd, J = 0.8, 7.6 Hz), 9.23 (1H, d, J = 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 21.2, 26.1, 27.4, 128.1, 131.4, 134.9, 137.2, 138.6, 140.2, 144.1, 146.6, 182.4, 183.3, 208.1. IR (KBr): ν = 1630, 1290, 760 and 610 cm−1. Anal. Calc. for C17H18BiClO: C, 42.30; H, 3.76. Found: C, 42.10; H, 3.82%.
(2-Butyrylphenyl)chloro(4-methylphenyl)bismuthane (11a). Yield: 21%; mp 136–138 °C; 1H-NMR (400 MHz, CDCl3): δ 0.94 (3H, t, J = 7.6 Hz), 1.75 (2H, m), 2.25 (3H, s), 3.02 (2H, m), 7.24 (2H, d, J = 7.6 Hz), 7.69 (1H, t, J = 7.6 Hz), 7.86 (1H, t, J = 7.6 Hz), 8.07 (2H, d, J = 7.6 Hz), 8.24 (1H, d, J = 7.6 Hz), 9.41 (1H, d, J = 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 13.7, 17.9, 21.5, 41.2, 128.1, 132.4, 134.5, 136.4, 137.9, 138.0, 138.4, 143.0, 178.0, 185.5, 211.6. IR (KBr): ν = 1620, 1220, 800, 760 and 480 cm−1. Anal. Calc. for C17H18BiClO: C, 42.30; H, 3.76. Found: C, 41.89; H, 3.69%.
Chloro(2-valerylphenyl)(4-methylphenyl)bismuthane (11b). Yield: 21%; mp 133–135 °C; 1H-NMR (400 MHz, CDCl3): δ 0.92 (3H, t, J = 7.6 Hz), 1.34 (2H, m), 1.70 (2H, m), 2.24 (3H, s), 3.05 (2H, m), 7.32 (2H, d, J = 7.6 Hz), 7.61 (1H, dt, J = 1.2, 7.6 Hz), 7.95 (1H, dt, J = 1.2, 7.6 Hz), 8.02 (2H, d, J = 7.6 Hz), 8.26 (1H, d, J = 7.6 Hz), 9.06 (1H, dd, J = 0.8, 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ13.8, 21.5, 22.3, 26.5, 39.1, 128.1, 132.4, 134.6, 136.4, 137.9, 138.1, 138.4, 143.0, 178.0, 185.5, 211.8. IR (KBr): ν = 1630, 1380, 1010, 800, 740 and 480 cm−1. Anal. Calc. for C18H20BiClO: C, 43.52; H, 4.06. Found: C, 43.88; H, 4.24%.
Bromo(2-isobutyrylphenyl)(4-methylphenyl)bismuthane (11c). Yield: 25%; mp 146–148 °C; 1H-NMR (400 MHz, DMSO-d6): δ 1.11 (6H, br-d, J = 4.1 Hz), 2.17 (3H, s), 3.89 (1H, sep, J = 6.8 Hz), 7.28 (2H, d, J = 7.6 Hz), 7.74 (1H, t, J = 7.6 Hz), 7.98 (2H, d, J = 7.6 Hz), 8.25 (1H, d, J = 7.6 Hz), 8.56 (1H, d, J = 7.6 Hz), 8.97 (1H, br-s). 13C-NMR (100 MHz, CDCl3): δ 19.1, 19.3, 21.5, 36.3, 128.2, 132.3, 134.3, 136.8, 137.9, 138.2, 140.9, 142.0, 174.4, 181.9, 215.1. IR (KBr): ν = 1620, 1260, 980, 770, 730 and 480 cm−1. Anal. Calc. for C17H18BiBrO: C, 38.73; H, 3.44. Found: C, 39.00; H, 3.63%.

3.4. Synthesis of 5b–e and 9

A typical example is exemplified by the synthesis of 5b: Compound 4b (510 mg, 1 mmol) was dissolved in dichloromethane (5 mL) and boron trifluoride etherate (3 mmol) was added to the solution at 0 °C until 4b was completely consumed (checked by TLC). The mixture was diluted by the addition of brine (5 mL) and the organic layer was extracted with ethyl acetate (20 mL × 3). The combined extracts were concentrated to leave an oily residue, which was crystallized from MeOH to give 5b in 88% yield.
(2-Acetylphenyl)chloro(4-methylphenyl)bismuthane (5b). Yield: 88%; mp 164–166 °C; 1H-NMR (400 MHz, CDCl3): δ 2.25 (3H, s), 2.69 (3H, s), 7.33 (2H, d, J = 7.6 Hz), 7.62 (1H, dt, J = 1.2, 7.6 Hz), 7.99 (1H, dt, J = 1.2, 7.6 Hz), 8.03 (2H, d, J = 7.6 Hz), 8.24 (1H, dd, J = 0.8, 7.6 Hz), 9.06 (1H, dd, J = 0.8, 7.2 Hz). 13C-NMR (100 MHz, CDCl3): δ 21.5, 27.4, 128.2, 132.4, 135.2, 136.4, 138.1, 138.3, 143.1, 177.9, 185.2, 209.2; one ipso carbon signal was too weak to be observed. IR (KBr): ν = 1625, 1550, 1280, 800, 765, 620 and 480 cm−1. Anal. Calc. for C15H14BiClO: C, 39.62; H, 3.10. Found: C, 39.60; H, 3.10%.
(2-Acetylphenyl)chloro(phenyl)bismuthane (5c). Yield: 82%; mp 152–154 °C; 1H-NMR (400 MHz, CDCl3): δ 2.69 (3H, s), 7.26 (1H, t, J = 7.8 Hz), 7.52 (2H, t, J = 8.0 Hz), 7.63 (1H, dt, J = 1.2, 7.6 Hz), 8.01 (1H, dt, J = 1.2, 7.6 Hz), 8.15 (2H, dd, J = 1.2, 8.0 Hz), 8.25 (1H, dd, J = 0.8, 7.2 Hz), 9.07 (1H, dd, J = 0.4, 8.0 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.4, 128.2, 128.3, 131.6, 135.2, 136.4, 136.2, 138.3, 143.2, 181.0, 185.3, 209.2. IR (KBr): ν = 1620, 1220, 800, 740 and 480 cm−1. Anal. Calc. for C14H12BiClO: C, 38.16; H, 2.74. Found: C, 37.94; H, 2.80%.
(2-Acetylphenyl)chloro(4-fluorophenyl)bismuthane (5d). Yield: 79%; mp 130–132 °C; 1H-NMR (400 MHz, CDCl3): δ 2.70 (3H, s), 7.11–7.15 (2H, m), 7.64 (1H, dt, J = 1.2, 7.6 Hz), 8.01 (1H, dt, J = 1.2, 7.6 Hz), 8.07–8.12 (2H, m), 8.26 (1H, dd, J = 0.4, 7.6 Hz), 9.04 (1H, d, J = 7.2 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.4, 118.8 (d, JCF = 20 Hz), 128.5, 135.3, 138.2, 138.3, 138.5 (d, JCF = 7.0 Hz), 143.1, 162.5 (d, JCF = 246 Hz), 175.9, 185.1, 209.5. IR (KBr): ν = 1630, 1570, 1480, 1220, 1160, 830, 780, 610 and 500 cm−1. Anal. Calc. for C14H11BiClFO: C, 36.66; H, 2.42. Found: C, 36.73; H, 2.37%.
(2-Acetylphenyl)chloro(4-chlorophenyl)bismuthane (5e). Yield: 91%; mp 168–170 °C; 1H-NMR (400 MHz, CDCl3): δ 2.70 (6H, s), 7.43 (2H, d, J = 8.0 Hz), 7.64 (1H, dt, J = 1.2, 7.6 Hz), 8.02 (1H, dt, J = 1.2, 7.6 Hz), 8.08 (2H, d, J = 8.0 Hz), 8.26 (1H, dd, J = 0.8, 8.0 Hz), 9.05 (1H, dd, J = 0.8, 7.2 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.4, 128.5, 131.7, 134.3, 135.4, 137.9, 138.2, 138.4, 143.1, 178.7, 185.5, 209.5. IR (KBr): ν = 1630, 1280, 1090, 1080, 810, 770 and 480 cm−1. Anal. Calc. for C14H11BiCl2O: C, 35.39; H, 2.33. Found: C, 35.45; H, 2.45%.
(2-Acetyl-4-methylphenyl)chloro(4-methylphenyl)bismuthane (9a). Yield: 87%; mp 196–198 °C; 1H-NMR (400 MHz, CDCl3): δ 2.25 (3H, s), 2.52 (3H, s), 2.66 (3H s), 7.30 (2H, d, J = 7.6 Hz), 7.40 (1H, d, J = 7.6 Hz), 8.03 (2H, d, J = 7.6 Hz), 8.13 (1H, d, J = 7.6 Hz), 8.13 (1H, d, J = 7.6 Hz), 8.85 (1H, s). 13C-NMR (100 MHz, CDCl3): δ21.5, 22.3, 27.3, 129.1, 132.4, 135.3, 136.4, 138.1, 139.0, 140.9, 149.8, 177.8, 185.6, 208.7. IR (KBr): ν = 1620, 1580, 1380, 800, 590 and 480 cm−1. Anal. Calc. for C16H16BiClO: C, 41.00; H, 3.44. Found: C, 40.78; H, 3.42%.
(2-Acetyl-4-isopropylphenyl)chloro(4-methylphenyl)bismuthane (9b). Yield: 93%; mp 198–200 °C; 1H-NMR (400 MHz, CDCl3): δ 1.33 (6H, d, J = 7.2 Hz), 2.28 (3H, s), 2.65 (3H, s), 3.13 (1H, sep, J = 7.2 Hz), 7.33 (2H, d, J = 7.6 Hz), 7.45 (1H, dd, J = 1.6, 8.0 Hz), 8.04 (2H, d, J = 7.6 Hz), 8.18 (1H, d, J = 8.0 Hz), 8.92 (1H, d, J = 1.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 21.5, 23.5, 23.7, 27.3, 34.9, 126.2, 132.4, 135.6, 136.4, 136.8, 138.1, 141.3, 159.9, 177.8, 185.7, 208.6. IR (KBr): ν = 1620, 1580, 1360, 1280, 1060, 830, 790 and 480 cm−1. Anal. Calc. for C18H20BiClO: C, 43.52; H, 4.06. Found: C, 43.48; H, 4.12%.
(2-Acetyl-4-methoxyphenyl)chloro(4-methylphenyl)bismuthane (9c). Yield: 82%; mp 178–180 °C; 1H-NMR (400 MHz, CDCl3): δ 2.25 (3H, s), 2.61 (3H, s), 4.01 (3H, s), 7.00 (1H, dd, J = 2.4, 8.8 Hz), 7.34 (2H, d, J = 7.6 Hz), 8.04 (2H, d, J = 7.6 Hz), 8.16 (1H, d, J = 8.8 Hz), 8.70 (1H, d, J = 2.4 Hz). 13C-NMR (100 MHz, CDCl3): δ21.5, 27.1, 55.8, 115.2, 122.6, 132.5, 136.4 (2C), 137.5, 138.2, 168.8, 178.1, 189.3, 207.0. IR (KBr): ν = 1610, 1580, 1540, 1360, 1300, 1270, 1230, 1020, 820, 800, 600 and 480 cm−1. Anal. Calc. for C16H16BiClO2: C, 39.65; H, 3.33. Found: C, 39.57; H, 3.73%.

3.5. Synthesis of 6 and 7

A typical example is exemplified by the synthesis of 6b and 7b: Saturated aqueous NaBr (3 mL) was added dropwise to a stirred solution of 5b (227 mg, 0.5 mmol) in dichloromethane (5 mL). After 15 min, the organic layer was extracted with ethyl acetate (10 mL × 3). The combined extracts were concentrated to leave an oily residue, which was crystallized from MeOH to give 6b in 83% yield. Saturated aqueous NaI (3 mL) was added dropwise to a stirred solution of 5b (227 mg, 0.5 mmol) in dichloromethane (5 mL). After 15 min, the organic layer was extracted with ethyl acetate (10 mL × 3). The combined extracts were concentrated to leave an oily residue, which was crystallized from MeOH to give 7b in 89% yield.
(2-Acetylphenyl)bromo(4-methoxylphenyl)bismuthane (6a). Yield: 85%; mp 153–155 °C; 1H-NMR (400 MHz, CDCl3): δ 2.69 (3H, s), 3.73 (3H, s), 6.98 (2H, d, J = 8.4 Hz), 7.66 (1H, dt, J = 1.2, 7.8 Hz), 7.97 (1H, dt, J = 1.2, 7.6 Hz), 8.02 (2H, d, J = 8.4 Hz), 8.25 (1H, d, J = 7.6 Hz), 9.21 (1H, d, J = 7.2 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.4, 55.1, 117.5, 128.4, 135.1, 138.5, 138.7, 140.7, 143.1, 159.4, 169.0, 180.7, 208.7. IR (KBr): ν = 1625, 1550, 1300, 1280, 800, 765, 615 and 480 cm−1. Anal. Calc. for C15H14BiBrO2: C, 34.97; H, 2.74. Found: C, 34.70; H, 2.70%.
(2-Acetylphenyl)bromo(4-methylphenyl)bismuthane (6b). Yield: 83%; mp 174–176 °C; 1H-NMR (400 MHz, CDCl3): δ 2.05 (3H, s), 2.69 (3H, s), 7.31 (2H, d, J = 7.9 Hz), 7.65 (1H, t, J = 1.1, 7.5 Hz), 7.97 (1H, t, J = 1.1, 7.4 Hz), 8.05 (2H, d, J = 7.9 Hz), 8.24 (1H, d, J = 7.3 Hz), 9.21 (1H, d, J = 7.3 Hz). 13C-NMR (100 MHz, CDCl3): δ 21.5, 27.4, 128.3, 132.5, 135.1, 137.1, 138.2, 138.5, 140.7, 143.1, 174.2, 175.8, 208.7. IR (KBr): ν = 1625, 1550, 1300, 1280, 800, 770, 620 and 480 cm−1. Anal. Calc. for C13H10BiClO2S: C, 36.09; H, 2.83. Found: C, 36.20; H, 2.80%.
(2-Acetylphenyl)bromo(phenyl)bismuthane (6c). Yield: 87%; mp 146–148 °C; 1H-NMR (400 MHz, CDCl3): δ2.70 (3H, s), 7.25 (1H, t, J = 7.3 Hz), 7.50 (2H, t, J = 7.3 Hz), 7.67 (1H, t, J = 7.3 Hz), 7.98 (1H, t, J = 7.3 Hz), 8.25 (1H, d, J = 7.3 Hz), 9.21 (1H, d, J = 7.3 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.4, 128.2, 128.4, 131.7, 135.1, 137.1, 138.6, 140.7, 143.1, 177.3, 180.8, 208.8. IR (KBr): ν = 1620, 1280, 770 and 730 cm−1. Anal. Calc. for C14H12BiBrO: C, 34.66; H, 2.48. Found: C, 34.80; H, 2.40%.
(2-Acetylphenyl)bromo(4-fluorophenyl)bismuthane (6d). Yield: 83%; mp 132–134 °C; 1H-NMR (400 MHz, CDCl3): δ 2.70, (3H, s), 7.09–7.13 (2H, m), 7.68 (1H, t, J = 7.2 Hz), 7.99 (1H, t, J = 7.6 Hz), 8.10–8.13 (2H, m), 8.26 (1H, J = 7.6 Hz), 9.20 (1H, d, J = 7.2 Hz). 13C-NMR (100 MHz, CDCl3): δ27.4, 118.9 (d, JCF = 21 Hz), 128.6, 135.1, 138.7, 139.2 (d, JCF = 7.0 Hz), 140.6, 143.1, 162.4 (d, JCF = 246 Hz), 172.0, 180.5, 208.9. IR (KBr): ν = 1630, 1220, 1160, 810, 610 and 500 cm−1. Anal. Calc. for C14H11BiBrFO: C, 33.42; H, 2.20. Found: C, 33.55; H, 2.24%.
(2-Acetylphenyl)bromo(4-chlorophenyl)bismuthane (6e). Yield: 86%; mp 157–159 °C; 1H-NMR (400 MHz, CDCl3): δ 2.70 (3H, s), 7.41 (2H, d, J = 8.4 Hz), 7.68 (1H, t, J = 7.6 Hz), 7.99 (1H, t, J = 8.0 Hz), 8.10 (2H, d, J = 8.4 Hz), 8.25 (1H, d, J = 7.6 Hz), 9.20 (1H, d, J = 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.4, 128.6, 131.7, 134.3, 135.2, 138.6, 138.7, 140.6, 143.1, 174.9, 180.4, 209.0. IR (KBr): ν = 1630, 1280, 1090, 1000, 800 and 770 cm−1. Anal. Calc. for C14H11BiBrClO: C, 32.36; H, 2.13. Found: C, 32.70; H, 2.10%.
(2-Acetylphenyl)bromo(2,4,6-trimethylphenyl)bismuthane (6f). Yield: 86%; mp 181–183 °C; 1H-NMR (400 MHz, CDCl3): δ 2.19 (3H, s), 2.45 (6H, s), 2.63 (3H, s), 7.12 (2H, s), 7.64 (1H, dt, J = 1.2, 7.6 Hz), 7.94 (1H, dt, J = 0.8, 7.2 Hz), 8.21 (1H, d, J = 7.2 Hz), 9.35 (1H, d, J = 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 21.2, 26.8, 27.3, 129.1, 131.2, 134.7, 137.6, 138.6, 142.2, 144.0, 146.6, 178.9, 179.3, 207.7. IR (KBr): ν = 1630, 1280, 1260 and 760 cm−1. Anal. Calc. for C17H18BiBrO: C, 38.73; H, 3.44. Found: C, 38.48; H, 3.55%.
(2-Acetylphenyl)iodo(4-methoxylphenyl)bismuthane (7a). Yield: 83%; mp 145–147 °C; 1H-NMR (400 MHz, CDCl3): δ 2.69 (3H, s), 3.73 (3H, s), 6.93 (2H, d, J = 8.4 Hz), 7.71 (1H, t, J = 7.6 Hz), 7.87 (1H, t, J = 7.2 Hz), 8.07 (2H, d, J = 8.4 Hz), 8.23 (1H, d, J = 7.6 Hz), 9.42 (1H, d, J = 7.2 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.1, 55.0, 117.4, 128.5, 134.5, 139.0, 139.9, 143.1, 145.6, 159.4, 207.5; ipso carbon signals were too weak to be observed. IR (KBr): ν = 1630, 1580, 1490, 1290, 1250, 1180, 810, 760 and 510 cm−1. Anal. Calc. for C14H13BiClNO2S: C, 32.05; H, 2.51. Found: C, 32.29; H, 2.60%.
(2-Acetylphenyl)iodo(4-methylphenyl)bismuthane (7b). Yield: 89%; mp 148–150 °C; 1H-NMR (400 MHz, CDCl3): δ2.26 (3H, s), 2.69 (3H, s), 7.25 (2H, d, J = 8.0 Hz), 7.71 (1H, dt, J = 1.2, 7.6 Hz), 7.86 (1H, dt, J = 1.6, 7.9 Hz), 8.07 (2H, d, J = 8.0 Hz), 8.22 (1H, dd, J = 1.2, 8.0 Hz), 9.42 (1H, dd, J = 1.2, 7.2 Hz). 13C-NMR (100 MHz, CDCl3): δ 21.6, 27.1, 128.5, 132.4, 134.5, 138.0, 138.2, 139.0, 143.1, 145.6, 166.7, 172.1, 207.5. IR (KBr): ν = 1630, 1290, 800, 770 and 480 cm−1. Anal. Calc. for C15H14BiIO: C, 32.98; H, 2.59. Found: C, 33.00; H, 2.60%.
(2-Acetylphenyl)iodo(phenyl)bismuthane (7c). Yield: 88%; mp 111–113 °C; 1H-NMR (400 MHz, CDCl3): δ 2.69 (3H, s), 7.25 (1H, dt, J = 0.8 Hz, 6.4 Hz), 7.37 (2H, dt, J = 1.6, 7.6 Hz), 7.89 (1H, dt, J = 1.2, 7.6 Hz), 8.20 (2H, dd, J = 1.2, 7.6 Hz), 8.23 (1H, dd, J = 1.2, 8.0 Hz), 9.43 (1H, dd, J = 0.8, 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.0, 128.0, 128.6, 131.5, 134.5, 138.2, 139.0, 143.1, 145.5, 169.8, 172.1, 207.6. IR (KBr): ν = 1630, 1430, 1280, 770, 730, 690, 610 and 460 cm−1. Anal. Calc. for C14H12BiIO: C, 31.60; H, 2.27. Found: C, 31.78; H, 2.60%.
(2-Acetylphenyl)iodo(4-fluorophenyl)bismuthane (7d). Yield: 76%; mp 123–125 °C; 1H-NMR (400 MHz, CDCl3): δ 2.70 (3H, s), 7.03-7.08 (2H, m), 7.73 (1H, dt, J = 0.8, 7.2 Hz), 7.90 (1H, dt, J = 0.8, 7.2 Hz), 8.11-8.16 (2H, m), 9.42 (1H, dd, J = 1.2, 7.6 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.1, 118.8 (d, JCF = 20 Hz), 128.7, 134.6, 139.1, 140.4 (d, JCF = 8.0 Hz), 143.0, 145.5, 162.4 (d, JCF = 246 Hz), 164.2, 171.7, 207.7. IR (KBr): ν = 1630, 1220, 1160, 810, 760, 610 and 500 cm−1. Anal. Calc. for C14H11BiFIO: C, 30.57; H, 2.02. Found: C, 30.52; H, 1.99%.
(2-Acetylphenyl)iodo(4-chlorophenyl)bismuthane (7e). Yield: 79%; mp 153–155 °C; 1H-NMR (400 MHz, CDCl3): δ 2.70 (3H, s), 7.43 (2H, dd, J = 1.6, 8.4 Hz), 7.73 (1H, dt, J = 1.2, 7.6 Hz), 7.90 (1H, dt, J = 1.2, 7.2 Hz), 8.11 (2H, dd, J = 1.6, 8.4 Hz), 8.24 (1H, dd, J = 0.8, 7.6 Hz), 9.41 (1H, dd, J = 0.8, 7.2 Hz). 13C-NMR (100 MHz, CDCl3): δ 27.1, 128.8, 131.6, 134.2, 134.6, 139.2, 139.8, 143.1, 145.5, 207.7; ipso carbon signals were too weak to be observed. IR (KBr): ν = 1630, 1470, 1280, 1090, 1000, 760, 610 and 480 cm−1. Anal. Calc. for C14H11BiClIO: C, 29.68; H, 1.96. Found: C, 29.72; H, 2.03%.
(2-Acetylphenyl)iodo(2,4,6-trimethylphenyl)bismuthane (7f). Yield: 86%; mp 170–172 °C; 1H-NMR (400 MHz, CDCl3): δ 2.20 (3H, s), 2.42 (6H, s), 2.63 (3H, s), 7.15 (2H, s), 7.67 (1H, dt, J = 0.8, 8.4 Hz), 7.84 (1H, dt, J = 1.2, 7.6 Hz), 8.19 (1H, d, J = 6.8 Hz), 9.52 (1H, d, J = 6.8 Hz). 13C-NMR (100 MHz, CDCl3): δ 21.2, 27.1, 27.9, 128.3, 130.7, 134.2, 138.1, 138.6, 144.0, 146.4, 146.6, 170.6, 172.8, 206.7. IR (KBr): ν = 1630, 1290, 1270, 850, 770 and 610 cm−1. Anal. Calc. for C17H18BiIO: C, 35.56; H, 3.16. Found: C, 35.56; H, 3.26%.

3.6. Qualitative Antifungal Assay

The yeast S. cerevisiae W303-1A (MATa ade2-1 can1-100 ura3-1 leu2-3,112 trp1-1 his3-11,15) was used for the qualitative antifungal assay. Yeast extract-peptone-dextrose (YPD) plates contained 1% yeast extract, 2% peptone, 2% glucose and 1.2% agar. The cells were inoculated at a concentration of 1.3 × 104 cells/mL in YPD agar medium at 48 °C and YPD plates were immediately made in Petri dishes. Each compound was dissolved in dimethyl sulfoxide (DMSO) at a concentration of 30 mM and 5 μL of each solution was directly spotted on the surface of the plate. The plates were incubated for 48 h at 30 °C and antifungal activity was indicated by the presence of clear inhibition zones around the spot. The control experiment showed that DMSO did not inhibit fungal growth at all. In order to know the error on the inhibition zone, we carried out the antifungal assay of compound C1 many times and confirmed that the error was within ± 1 mm.

3.7. Lipophilicity

The calculated logarithms of water-octanol partition coefficients (ClogP values) were obtained from the ClogP tool in Chem Draw Ultra 11.0 (Cambridge Soft, Cambridge, MA, USA).

3.8. DFT Calculation of the Dissociation and Association Energies

The geometries of the bismuth compounds, their dissociated cations and counter anions, and the corresponding MeS−-adduct anions in Table 2 were fully optimized in water through density functional theory (DFT) calculations within the polarizable continuum model (PCM) using the Gaussian 09 program package [40]. The hybrid B3LYP exchange-correlation functional and 6-31+G*/lanl2dz mixed basis set (lanl2dz effective core potential for bismuth and iodine and 6-31+G* basis set for the remaining atoms) were employed. All d functions in 6-31+G* are pure 5 D basis functions, which is the default form in the Gaussian 09 GenECP calculations. The endothermicity for the ionic dissociation and the exothermicity for the nucleophilic addition of MeS− were calculated from the energies in water of each substrate, in which only the most stable conformer with the lowest energy was considered.

4. Conclusions

In conclusion, the present study provides insights that will aid the design of fungicidal hypervalent bismuthanes. For higher antifungal activity, adjusting the lipophilicity-hydrophilicity balance, modeling the three-dimensional molecular structure around the bismuth atom, and stabilizing the intermediate ate complex in Path 2 appeared to be more important than tuning the Lewis acidity at the bismuth atom. Further studies are now underway to test this hypothesis.

Acknowledgments

We are grateful to the Center of Instrumental Analysis, Yamaguchi University and the Tokiwa Instrumentation Analysis Center, Yamaguchi University.

Author Contributions

T.M. conceived the concept and design of this study and wrote the article. T.M. and M.T. synthesized and characterized compounds. I.M. performed the antifungal assay. K.I. analyzed the results and carried out the DFT calculation.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Onishi, K.; Douke, M.; Nakamura, T.; Ochiai, Y.; Kakusawa, N.; Yasuike, S.; Kurita, J.; Yamamoto, C.; Kawahata, M.; Yamaguchi, K.; et al. A novel organobismuth compound, 1-[(2-di-p-tolylbismuthanophenyl)diazenyl]pyrrolidine, induces apoptosis in the human acute promyelocytic leukemia cell line NB4 via reactive oxygen species. J. Inorg. Biochem. 2012, 117, 77–84. [Google Scholar] [CrossRef]
  2. Andrews, P.C.; Frank, R.; Junk, P.C.; Kedzierski, L.; Kumar, I.; MacLellan, J.G. Anti-Leishmanial activity of homo- and heteroleptic bismuth(III) carboxylates. J. Inorg. Biochem. 2011, 105, 454–461. [Google Scholar] [CrossRef]
  3. Iuchi, K.; Hatano, Y.; Yagura, T. Heterocyclic organobismuth(III) induces apoptosis of human promyelocytic leukemic cells through activation of caspases and mitochondrial perturbation. Biochem. Pharmacol. 2008, 76, 974–986. [Google Scholar] [CrossRef]
  4. Li, H.; Lai, C.S.; Wu, J.; Ho, P.C.; de Vos, D.; Tiekink, E.R.T. Cytotoxicity, qualitative structure-activity relationship (QSAR), and anti-tumor activity of bismuth dithiocarbamate complexes. J. Inorg. Biochem. 2007, 101, 809–816. [Google Scholar] [CrossRef]
  5. Islam, A.; da Silva, J.G.; Berbet, F.M.; da Silva, S.M.; Rodrigues, B.L.; Beraldo, H.; Melo, M.N.; Frézard, F.; Demicheli, C. Novel triphenylantimony(V) and triphenylbismuth(V) complexes with benzoic acid derivatives: Structural characterization, in vitro antileishmanial and antibacterial activities and cytotoxicity against macrophages. Molecules 2014, 19, 6009–6030. [Google Scholar] [CrossRef]
  6. Briand, G.G.; Burford, N. Bismuth compounds and preparations with biological or medicinal relevance. Chem. Rev. 1999, 99, 2601–2657. [Google Scholar] [CrossRef]
  7. Condon, S.; Pichon, C.; Davi, M. Preparation and synthetic applications of trivalent arylbismuth compounds as arylating reagents. A review. Org. Prep. Proced. Int. 2014, 46, 89–131. [Google Scholar] [CrossRef]
  8. Shimada, S.; Rao, M.L.N. Transition-metal catalyzed C-C bond formation using organobismuth compounds. Top. Curr. Chem. 2012, 311, 199–228. [Google Scholar] [CrossRef]
  9. Braunschweig, H.; Cogswell, P.; Schwab, K. Synthesis, structure and reactivity of complexes containing a transition metal-bismuth bond. Coord. Chem. Rev. 2011, 255, 101–117. [Google Scholar] [CrossRef]
  10. Murafuji, T.; Miyoshi, Y.; Ishibashi, M.; Rahman, A.F.M.M.; Sugihara, Y.; Miyakawa, I.; Uno, H. Antifungal activity of organobismuth compounds against the yeast Saccharomyces cerevisiae: Structure-activity relationship. J. Inorg. Biochem. 2004, 98, 547–552. [Google Scholar] [CrossRef]
  11. Murafuji, T.; Fujiwara, Y.; Yoshimatsu, D.; Miyakawa, I.; Migita, K.; Mikata, Y. Bismuth heterocycles based on a diphenyl sulfone scaffold: Synthesis and substituent effect on the antifungal activity against Saccharomyces cerevisiae. Eur. J. Med. Chem. 2011, 46, 519–525. [Google Scholar] [CrossRef]
  12. Murafuji, T.; Kitagawa, K.; Yoshimatsu, D.; Kondo, K.; Ishiguro, K.; Tsunashima, R.; Miyakawa, I.; Mikata, Y. Heterocyclic bismuth carboxylates based on a diphenyl sulfone scaffold: Synthesis and antifungal activity against Saccharomyses cerevisiae. Eur. J. Med. Chem. 2013, 63, 531–535. [Google Scholar] [CrossRef]
  13. Chen, X.; Yamamoto, Y.; Akiba, K.-Y. Hypervalent tetracoordinate organobismuth compounds (10-Bi-4). Heteroatom Chem. 1995, 6, 293–303. [Google Scholar] [CrossRef]
  14. Ohkata, K.; Takemoto, S.; Ohnishi, M.; Akiba, K.-Y. Synthesis and chemical behaviors of 12-substituted dibenz[c,f][1,5]azastibocine and dibenz[c,f][1,5]azabismocine derivatives: Evidence of 10-Pn-4 type hypervalent interaction. Tetrahedron Lett. 1989, 30, 4841–4844. [Google Scholar]
  15. Qiu, R.; Yin, S.; Zhang, X.; Xia, J.; Xu, X.; Luo, S. Synthesis and structure of an air-stable cationic organobismuth complex and its use as a highly efficient catalyst for the direct diastereoselective Mannich reaction in water. Chem. Comm. 2009, 4759–4761. [Google Scholar]
  16. Bao, M.; Hayashi, T.; Shimada, S. Cationic organobismuth complex with 5,6,7,12-tetrahydrodibenz[c,f][1,5]azabismocine framework and its coordination complexes with neutral molecules. Organometallics 2007, 26, 1816–1822. [Google Scholar] [CrossRef]
  17. Rajabi, L.; Courreges, C.; Montoya, J.; Aguilera, R.J.; Primm, T.P. Acetophenones with selective antimycobacterial activity. Lett. Appl. Microbiol. 2005, 40, 212–217. [Google Scholar] [CrossRef]
  18. Sivakumar, P.M.; Sheshayan, G.; Doble, M. Experimental and QSAR of acetophenones as antibacterial agents. Chem. Biol. Drug. Des. 2008, 72, 303–313. [Google Scholar] [CrossRef]
  19. Li, Q.; Ning, P.; Zheng, L.; Huang, J.; Li, G.; Hsiang, T. Fumigant activity of volatiles of Streptomyces globisporus JK-1 against Penicillium italicum on Citrus microcarpa. Postharv. Biol. Technol. 2010, 58, 157–165. [Google Scholar] [CrossRef]
  20. Cazeau, P.; Duboudin, F.; Moulines, F.; Babot, O.; Dunogues, J. A new practical synthesis of silyl enol ethers: Part. 1. From simple aldehydes and ketones. Tetrahedron 1987, 43, 2075–2088. [Google Scholar] [CrossRef]
  21. Prantz, K.; Mulzer, J. Synthesis of (Z)-trisubstituted olefins by decarboxylative Grob-type fragmentations: Epothilone D, discodermolide, and peloruside A. Chem. Eur. J. 2010, 16, 485–506. [Google Scholar] [CrossRef]
  22. Murafuji, T.; Mutoh, T.; Satoh, K.; Tsunenari, K.; Azuma, N.; Suzuki, H. Hypervalent bond formation in halogeno(2-acylphenyl)bismuthanes. Organometallics 1995, 14, 3848–3854. [Google Scholar] [CrossRef]
  23. Klein, J.; Medlik-Balan, A. Metalation reactions. 18. Polymetalation substituted acetophenones. J. Org. Chem. 1976, 41, 3307–3312. [Google Scholar] [CrossRef]
  24. Suzuki, H.; Murafuji, T.; Azuma, N. Synthesis and first X-ray structure analysis of a stabilized chiral chlorobismuthine: Fixation of molecular geometry induced by the intramolecular coordination of a sulfonyl group. J. Chem. Soc. Perkin Trans. 1 1993, 1169–1175. [Google Scholar] [CrossRef]
  25. Compounds 5a, 6a, and 7a were partially deposited on the surface of the agar medium.
  26. Fujiwara, Y.; Mitani, M.; Yasuike, S.; Kurita, J.; Kaji, T. An organobismuth compound that exhibits selective cytotoxicity to vascular endothelial cells in vitro. J. Health Sci. 2005, 51, 333–340. [Google Scholar] [CrossRef]
  27. Jeschke, P. The unique role of halogen substituents in the design of modern agrochemicals. Pest. Manag. Sci. 2010, 66, 10–27. [Google Scholar] [CrossRef]
  28. Jeschke, P. The unique role of fluorine in the design of active ingredients for modern crop protection. ChemBioChem 2004, 5, 570–589. [Google Scholar] [CrossRef]
  29. Murphy, C.D.; Clark, B.R.; Amadio, J. Metabolism of fluoroorganic compounds in microorganisms: Impacts for the environment and the production of fine chemicals. Appl. Microbiol. Biotechnol. 2009, 84, 617–629. [Google Scholar] [CrossRef]
  30. Timoshkin, A.L.; Bodensteiner, M.; Sevastianova, T.N.; Lisovenko, A.S.; Davydova, E.I.; Scheer, M.; Graßl, C.; Butlak, A.V. Do solid-state structures reflect Lewis acidity trends of heavier group 13 trihalides? Experimental and theoretical case study. Inorg. Chem. 2012, 51, 11602–11611. [Google Scholar] [CrossRef]
  31. Dakternieks, D.; Jurkschat, K.; Tozer, R.; Hook, J.; Tiekink, E.R.T. Novel coordination isomerization in organotin(IV) compounds. Synthesis, molecular structures, and NMR studies of LSnPhX2 (X = Ph, Cl, Br, I, SPh), LCH2SnPhX2 (X = Ph, Cl, Br, I), and LSiPh3, where LH is (2-MeO-3-tBu-5-Me-C6H2)2CH2. Organometallics 1997, 16, 3696–3706. [Google Scholar] [CrossRef]
  32. Suzuki, H.; Murafuji, T.; Matano, Y.; Azuma, N. Chiral chlorobismuthines stabilized by the intramolecular coordination of an N,N-dimethylamino group: X-ray structure analysis, asymmetric induction at the bismuth centre, and dynamic behaviour in solution. J. Chem. Soc. Perkin Trans. 1 1993, 2969–2973. [Google Scholar]
  33. Beleaga, A.; Bojan, V.R.; Pöllnitz, A.; Raț, C.I.; Silvestru, C. Organomercury(II) and tellurium(II) compounds with the “pincer” ligand 2,6-[O(CH2CH2)2NCH2]2C6H3 - stabilization of an unusual organotellurium(II) cationic species. Dalton Trans. 2011, 40, 8830–8838. [Google Scholar] [CrossRef]
  34. Denmark, S.E.; Beutner, G.L. Lewis base catalysis in organic synthesis. Angew. Chem. Int. Ed. 2008, 47, 1560–1638. [Google Scholar] [CrossRef]
  35. Holmes, R.R. Comparison of phosphorus and silicon: Hypervalency, stereochemistry, and reactivity. Chem. Rev. 1996, 96, 927–950. [Google Scholar] [CrossRef]
  36. Chuit, C.; Corriu, R.J.P.; Reye, C.; Young, J.C. Reactivity of penta- and hexacoordinate silicon compounds and their role as reaction intermediates. Chem. Rev. 1993, 93, 1371–1448. [Google Scholar] [CrossRef]
  37. Trinquier, G.; Daudey, J.-P.; Caruana, G.; Madaule, Y. Theoretical data on the multicoordination of phosphorus and arsenic. J. Am. Chem. Soc. 1984, 106, 4794–4799. [Google Scholar] [CrossRef]
  38. Deiters, J.A.; Holmes, R.R. Enhanced reactivity of pentacoordinated silicon species. An ab initio approach. J. Am. Chem. Soc. 1990, 112, 7197–7202. [Google Scholar] [CrossRef]
  39. Burford, N.; Eelman, M.D.; Mahony, D.E.; Morash, M. Definitive identification of cysteine and glutathione complexes of bismuth by mass spectrometry: Assessing the biochemical fate of bismuth pharmaceutical agents. Chem. Comm. 2003, 146–147. [Google Scholar]
  40. Frisch, M.J.; Trucks, G.W.; Schlegel, H.B.; Scuseria, G.E.; Robb, M.A.; Cheeseman, J.R.; Scalmani, G.; Barone, V.; Mennucci, B.; Petersson, G.A.; et al. Gaussian 09; Revision C.01 Gaussian,Inc.: Wallingford, CT, USA, 2010. [Google Scholar]
  41. Kolb, U.; Dräger, M.; Jousseaume, B. Triorganotin compounds SnXMe2-1,4-cyclohexadiene-COOMe: Intra- and intermolecular unfolding of an inner tetrahedron to distorted trigonal bipyramids (X = Cl, Br, I) and a distorted octahedron (X = F). Organometallics 1991, 10, 2737–2742. [Google Scholar] [CrossRef]
  • Sample Availability: Samples of the compounds A-D and 4-11 are available from the authors.

Share and Cite

MDPI and ACS Style

Murafuji, T.; Tomura, M.; Ishiguro, K.; Miyakawa, I. Activity of Antifungal Organobismuth(III) Compounds Derived from Alkyl Aryl Ketones against S. cerevisiae: Comparison with a Heterocyclic Bismuth Scaffold Consisting of a Diphenyl Sulfone. Molecules 2014, 19, 11077-11095. https://doi.org/10.3390/molecules190811077

AMA Style

Murafuji T, Tomura M, Ishiguro K, Miyakawa I. Activity of Antifungal Organobismuth(III) Compounds Derived from Alkyl Aryl Ketones against S. cerevisiae: Comparison with a Heterocyclic Bismuth Scaffold Consisting of a Diphenyl Sulfone. Molecules. 2014; 19(8):11077-11095. https://doi.org/10.3390/molecules190811077

Chicago/Turabian Style

Murafuji, Toshihiro, Mai Tomura, Katsuya Ishiguro, and Isamu Miyakawa. 2014. "Activity of Antifungal Organobismuth(III) Compounds Derived from Alkyl Aryl Ketones against S. cerevisiae: Comparison with a Heterocyclic Bismuth Scaffold Consisting of a Diphenyl Sulfone" Molecules 19, no. 8: 11077-11095. https://doi.org/10.3390/molecules190811077

Article Metrics

Back to TopTop