An Isoflavone from Dipteryx alata Vogel is Active against the in Vitro Neuromuscular Paralysis of Bothrops jararacussu Snake Venom and Bothropstoxin I, and Prevents Venom-Induced Myonecrosis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Molecule
2.2. Pharmacological Assays
2.3. Quantitative Histological Analysis
2.4. Salmonella Mutagenicity Assay
TM | ||||
---|---|---|---|---|
Treatment | TA 98 | TA 97a | ||
mg/plate | −S9 | +S9 | −S9 | +S9 |
DMSO | 20 ± 2 | 30 ± 2 | 151 ± 8 | 143 ± 8 |
0.19 | 20 ± 4 (1.0) | 34 ± 3 (1.1) | 194 ± 4 (1.3) | 172 ± 4 (1.2) |
Control+ | 1319 ± 41 a | 1696 ± 41 b | 1875 ± 62 a | 1623 ± 48 b |
3. Experimental
3.1. Plant Material and Extraction
3.1.1. Isolation of 7,8,3'-trihydroxy-4'-methoxyisoflavone
3.1.2. Isoflavone Solubilization
3.2. Pharmacological Assays
3.2.1. Venom and Purification of BthTX-I
3.2.2. Animals
3.2.3. Mouse Phrenic Nerve-Diaphragm Muscle (PND) Preparation
3.2.4. Quantitative Histological Analysis
3.2.5. In vitro Mutagenicity Assay
3.2.6. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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- Sample Availability: Not available.
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Ferraz, M.C.; Yoshida, E.H.; Tavares, R.V.S.; Cogo, J.C.; Cintra, A.C.O.; Dal Belo, C.A.; Franco, L.M.; Dos Santos, M.G.; Resende, F.A.; Varanda, E.A.; et al. An Isoflavone from Dipteryx alata Vogel is Active against the in Vitro Neuromuscular Paralysis of Bothrops jararacussu Snake Venom and Bothropstoxin I, and Prevents Venom-Induced Myonecrosis. Molecules 2014, 19, 5790-5805. https://doi.org/10.3390/molecules19055790
Ferraz MC, Yoshida EH, Tavares RVS, Cogo JC, Cintra ACO, Dal Belo CA, Franco LM, Dos Santos MG, Resende FA, Varanda EA, et al. An Isoflavone from Dipteryx alata Vogel is Active against the in Vitro Neuromuscular Paralysis of Bothrops jararacussu Snake Venom and Bothropstoxin I, and Prevents Venom-Induced Myonecrosis. Molecules. 2014; 19(5):5790-5805. https://doi.org/10.3390/molecules19055790
Chicago/Turabian StyleFerraz, Miriéle C., Edson H. Yoshida, Renata V.S. Tavares, José C. Cogo, Adélia C.O. Cintra, Cháriston A. Dal Belo, Luiz M. Franco, Márcio G. Dos Santos, Flávia A. Resende, Eliana A. Varanda, and et al. 2014. "An Isoflavone from Dipteryx alata Vogel is Active against the in Vitro Neuromuscular Paralysis of Bothrops jararacussu Snake Venom and Bothropstoxin I, and Prevents Venom-Induced Myonecrosis" Molecules 19, no. 5: 5790-5805. https://doi.org/10.3390/molecules19055790