Fluorescent Probes for Insect Ryanodine Receptors: Candidate Anthranilic Diamides
Abstract
:1. Introduction
2. Results and Discussion
2.1. Fluorescent Ligands Design and Synthesis
2.2. Fluorescent Ligands Bind Potently to Specific Site in Musca RyR
2.3. Insecticidal Activity of Fluorescent Ligands
Compd | LC50 (95%CL) a (ppm) | Slope SE | x2 (df) b |
---|---|---|---|
Chlo | 0.033(0.006–0.059) | 2.5 ± 0.5 | 13.7(6) |
Cyan | 0.061(0.035–0.086) | 5.0 ± 1.0 | 14.5(8) |
Flu | 0.056(0.037–0.068) | 6.9 ± 2.1 | 1.7(7) |
Prop-Cl | 0.080(0.024–0.152) | 1.5 ± 0.3 | 5.5 (7) |
Prop-CN | 0.34(0.24–0.43) | 4.6 ± 1.1 | 7.9(8) |
Fluor-Cl | 2.59(1.69–3.46) | 4.0 ± 0.9 | 5.2(8) |
Fluor-CN | 7.57(4.57–11.77) | 2.4 ± 0.7 | 3.8(8) |
3. Experimental
3.1. General Methods and Materials
3.2. Synthesis of Propynyl Intermediates
3.3. Synthesis of Fluorescent Ligands
3.4. Insecticidal Activity
3.5. RyR Radioligand Assay
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Wang, Y.; Guo, L.; Qi, S.; Zhang, H.; Liu, K.; Liu, R.; Liang, P.; Casida, J.E.; Liu, S. Fluorescent Probes for Insect Ryanodine Receptors: Candidate Anthranilic Diamides. Molecules 2014, 19, 4105-4114. https://doi.org/10.3390/molecules19044105
Wang Y, Guo L, Qi S, Zhang H, Liu K, Liu R, Liang P, Casida JE, Liu S. Fluorescent Probes for Insect Ryanodine Receptors: Candidate Anthranilic Diamides. Molecules. 2014; 19(4):4105-4114. https://doi.org/10.3390/molecules19044105
Chicago/Turabian StyleWang, Yi, Lei Guo, Suzhen Qi, Hao Zhang, Kechang Liu, Ruiquan Liu, Pei Liang, John E. Casida, and Shangzhong Liu. 2014. "Fluorescent Probes for Insect Ryanodine Receptors: Candidate Anthranilic Diamides" Molecules 19, no. 4: 4105-4114. https://doi.org/10.3390/molecules19044105