Next Article in Journal
Chemo-Enzymatic Synthesis of Silybin and 2,3-Dehydrosilybin Dimers
Previous Article in Journal
Simultaneous Determination of Original, Degraded Ginsenosides and Aglycones by Ultra High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry for Quantitative Evaluation of Du-Shen-Tang, the Decoction of Ginseng
Article Menu

Export Article

Open AccessArticle
Molecules 2014, 19(4), 4105-4114;

Fluorescent Probes for Insect Ryanodine Receptors: Candidate Anthranilic Diamides

Department of Applied Chemistry, College of Science, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China
College of Agriculture and Biotechnology, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China
Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA 94720-3112, USA
Author to whom correspondence should be addressed.
Received: 16 January 2014 / Revised: 13 March 2014 / Accepted: 21 March 2014 / Published: 2 April 2014
Full-Text   |   PDF [632 KB, uploaded 18 June 2014]   |  


Diamide insecticides with high efficacy against pests and good environmental safety are broadly applied in crop protection. They act at a poorly-defined site in the very complex ryanodine (Ry) receptor (RyR) potentially accessible to a fluorescent probe. Two N-propynyl analogs of the major anthranilic diamide insecticides chlorantraniliprole (Chlo) and cyantraniliprole (Cyan) were accordingly synthesized and converted into two fluorescent ligands by click reaction coupling with 3-azido-7-hydroxy-2H-chromen-2-one. The new diamide analogs and fluorescent ligands were shown to be nearly as potent as Chlo and Cyan in inhibition of [3H]Chlo binding and stimulation of [3H]Ry binding in house fly thoracic muscle RyR. Although the newly synthesized compounds had only moderate activity in insect larvicidal activity assays, their high in vitro potency in a validated insect RyR binding assay encourages further development of fluorescent probes for insect RyRs. View Full-Text
Keywords: fluorescent probe; affinity; ryanodine receptor fluorescent probe; affinity; ryanodine receptor

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Wang, Y.; Guo, L.; Qi, S.; Zhang, H.; Liu, K.; Liu, R.; Liang, P.; Casida, J.E.; Liu, S. Fluorescent Probes for Insect Ryanodine Receptors: Candidate Anthranilic Diamides. Molecules 2014, 19, 4105-4114.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top