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Molecular Modeling to Study Dendrimers for Biomedical Applications
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Molecules 2014, 19(12), 20731-20750;

Investigation of Dendriplexes by Ion Mobility-Mass Spectrometry

Normandie Université, COBRA, UMR6014 and FR3038, Université de Rouen, CNRS, IRCOF, 1 rue Tesnière, 76821 Mont-Saint-Aignan Cedex, France
School of Chemistry, University of Southampton, Highfield, Hants SO17 1BJ Southampton, UK
Author to whom correspondence should be addressed.
Received: 24 July 2014 / Revised: 22 October 2014 / Accepted: 27 October 2014 / Published: 12 December 2014
(This article belongs to the Special Issue Dendrimers in Medicine and Biotechnology)
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Highly branched polyamidoamine (PAMAM) dendrimers presenting biological activities have been envisaged as non-viral gene delivery vectors. They are known to associate with nucleic acid (DNA) in non-covalent complexes via electrostatic interactions. Although their transfection efficiency has been proved, PAMAMs present a significant cytotoxicity due to their cationic surface. To overcome such a drawback, different chemical modifications of the PAMAM surface have been reported such as the attachment of hydrophobic residues. In the present work, we studied the complexation of DNA duplexes with different low-generation PAMAM; ammonia-cored G0(N) and G1(N) PAMAM, native or chemically modified with aromatic residues, i.e., phenyl-modified-PAMAM G0(N) and phenylalanine-modified-PAMAM G1(N). To investigate the interactions involved in the PAMAM/DNA complexes, also called dendriplexes, we used electrospray ionization (ESI) coupled to ion mobility spectrometry-mass-spectrometry (IM-MS). ESI is known to allow the study of non-covalent complexes in native conditions while IM-MS is a bidimensional separation technique particularly useful for the characterization of complex mixtures. IM-MS allows the separation of the expected complexes, possible additional non-specific complexes and the free ligands. Tandem mass spectrometry (MS/MS) was also used for the structural characterization. This work highlights the contribution of IM-MS and MS/MS for the study of small dendriplexes. The stoichiometries of the complexes and the equilibrium dissociation constants were determined. The [DNA/native PAMAM] and [DNA/modified-PAMAM] dendriplexes were compared. View Full-Text
Keywords: polyamidoamine dendrimers; oligonucleotide duplex; dendriplexes; electrospray mass spectrometry; tandem mass spectrometry; ion mobility spectrometry polyamidoamine dendrimers; oligonucleotide duplex; dendriplexes; electrospray mass spectrometry; tandem mass spectrometry; ion mobility spectrometry

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Leriche, E.-D.; Hubert-Roux, M.; Afonso, C.; Lange, C.M.; Grossel, M.C.; Maire, F.; Loutelier-Bourhis, C. Investigation of Dendriplexes by Ion Mobility-Mass Spectrometry. Molecules 2014, 19, 20731-20750.

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