Next Article in Journal
Characterization of Water Extractable Arabinoxylans from a Spring Wheat Flour: Rheological Properties and Microstructure
Previous Article in Journal
Distinct Metabolites for Photoreactive l-Phenylalanine Derivatives in Klebsiella sp. CK6 Isolated from Rhizosphere of a Wild Dipterocarp Sapling
Article Menu

Export Article

Open AccessArticle
Molecules 2013, 18(7), 8402-8416;

Characterization of Flavonoids and Phenolic Acids in Myrcia bella Cambess. Using FIA-ESI-IT-MSn and HPLC-PAD-ESI-IT-MS Combined with NMR

Botany Department, Institute of Biosciences, Univ. Estadual Paulista (UNESP), CEP 18618-970, Botucatu, Sao Paulo, Brazil
Experimental Campus of the Paulista Coast, Univ. Estadual Paulista (UNESP), CEP 11330-900, Sao Vicente, Sao Paulo, Brazil
Biological Science Department, Science Faculty, Univ. Estadual Paulista (UNESP), CEP 17033-360, Bauru, Sao Paulo, Brazil
Author to whom correspondence should be addressed.
Received: 7 June 2013 / Revised: 5 July 2013 / Accepted: 8 July 2013 / Published: 16 July 2013
(This article belongs to the Section Natural Products Chemistry)
Full-Text   |   PDF [602 KB, uploaded 18 June 2014]   |  


The leaves of Myrcia DC. ex Guill species are used in traditional medicine and are also exploited commercially as herbal drugs for the treatment of diabetes mellitus. The present work aimed to assess the qualitative and quantitative profiles of M. bella hydroalcoholic extract, due to these uses, since the existing legislation in Brazil determines that a standard method must be developed in order to be used for quality control of raw plant materials. The current study identified eleven known flavonoid-O-glycosides and six acylated flavonoid derivatives of myricetin and quercetin, together with two kaempferol glycosides and phenolic acids such as caffeic acid, ethil galate, gallic acid and quinic acid. In total, 24 constituents were characterized, by means of extensive preparative chromatographic analyses, along with MS and NMR techniques. An HPLC-PAD-ESI-IT-MS and FIA-ESI-IT-MSn method were developed for rapid identification of acylated flavonoids, flavonoid-O-glycosides derivatives of myricetin and quercetin and phenolic acids in the hydroalcoholic M. bella leaves extract. The FIA-ESI-IT-MS techinique is a powerful tool for direct and rapid identification of the constituents after isolation and NMR characterization. Thus, it could be used as an initial method for identification of authentic samples concerning quality control of Myrcia spp extracts. View Full-Text
Keywords: Brazilian savanna; flavonoid-O-glycosides; medicinal plants; Myrtaceae Brazilian savanna; flavonoid-O-glycosides; medicinal plants; Myrtaceae

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Saldanha, L.L.; Vilegas, W.; Dokkedal, A.L. Characterization of Flavonoids and Phenolic Acids in Myrcia bella Cambess. Using FIA-ESI-IT-MSn and HPLC-PAD-ESI-IT-MS Combined with NMR. Molecules 2013, 18, 8402-8416.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top