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Molecules 2013, 18(11), 13831-13859;

Photoaffinity Labeling of Plasma Proteins

School of Pharmacy, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University, GPO Box U1987, Perth 6845, WA, Australia
Graduate School of Pharmaceutical Sciences, DDS Research Institute, Sojo University, Kumamoto 860-0082, Japan
Authors to whom correspondence should be addressed.
Received: 20 August 2013 / Revised: 22 October 2013 / Accepted: 4 November 2013 / Published: 8 November 2013
(This article belongs to the Special Issue Reagents and Methods for Protein Target Identification)
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Photoaffinity labeling is a powerful technique for identifying a target protein. A high degree of labeling specificity can be achieved with this method in comparison to chemical labeling. Human serum albumin (HSA) and α1-acid glycoprotein (AGP) are two plasma proteins that bind a variety of endogenous and exogenous substances. The ligand binding mechanism of these two proteins is complex. Fatty acids, which are known to be transported in plasma by HSA, cause conformational changes and participate in allosteric ligand binding to HSA. HSA undergoes an N-B transition, a conformational change at alkaline pH, that has been reported to result in increased ligand binding. Attempts have been made to investigate the impact of fatty acids and the N-B transition on ligand binding in HSA using ketoprofen and flunitrazepam as photolabeling agents. Meanwhile, plasma AGP is a mixture of genetic variants of the protein. The photolabeling of AGP with flunitrazepam has been utilized to shed light on the topology of the protein ligand binding site. Furthermore, a review of photoaffinity labeling performed on other major plasma proteins will also be discussed. Using a photoreactive natural ligand as a photolabeling agent to identify target protein in the plasma would reduce non-specific labeling. View Full-Text
Keywords: photoaffinity labeling; plasma proteins; human serum albumin; α1-acid glycoprotein; photoreactivity; protein binding; labeling specificity photoaffinity labeling; plasma proteins; human serum albumin; α1-acid glycoprotein; photoreactivity; protein binding; labeling specificity

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Chuang, V.T.G.; Otagiri, M. Photoaffinity Labeling of Plasma Proteins. Molecules 2013, 18, 13831-13859.

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