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Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography

1
College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China
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Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huaian 223300, China
3
Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian 223300, China
*
Author to whom correspondence should be addressed.
Molecules 2012, 17(8), 9002-9009; https://doi.org/10.3390/molecules17089002
Received: 20 June 2012 / Revised: 18 July 2012 / Accepted: 21 July 2012 / Published: 27 July 2012
A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane–ethyl acetate–methanol–water (1:4:2:3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3β-hydroxy-8β-[4'-hydroxy-tigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and 1H-NMR. View Full-Text
Keywords: Eupatorium lindleyanum DC.; high-speed counter-current chromatography; sesquiterpenoid lactone; separation Eupatorium lindleyanum DC.; high-speed counter-current chromatography; sesquiterpenoid lactone; separation
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Yan, G.; Ji, L.; Luo, Y.; Hu, Y. Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography. Molecules 2012, 17, 9002-9009.

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