Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography
Abstract
:1. Introduction
2. Results and Discussion
2.1. Selection of Two-Phase Solvent System and Other Separation Conditions of HSCCC
n-hexane-ethyl acetate-methanol-water | Partition coefficient (K) | ||
---|---|---|---|
(v/v/v/v) | K1 | K2 | K3 |
2:4:3:2 | 0.06 | 0.14 | 0.28 |
1:4:3:3 | 0.26 | 0.61 | 1.26 |
1:4:2:3 | 0.54 | 1.40 | 1.94 |
1:8:4:6 | 1.64 | 3.38 | 5.51 |
2.2. HSCCC Separation of Crude Sample
3. Experimental
3.1. Reagents and Materials
3.2. Apparatus
3.3. Plant Material
3.4. Preparation of Samples
3.5. Selection of Two-Phase Solvent System
3.6. Preparation of Two-Phase Solvent System and Sample Solution
3.7. HSCCC Separation Procedure
3.8. Analyses and Identification of HSCCC Peak Fractions
3.9. Structural Identification
4. Conclusions
Supplementary Materials
Acknowledgments
References
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Yan, G.; Ji, L.; Luo, Y.; Hu, Y. Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography. Molecules 2012, 17, 9002-9009. https://doi.org/10.3390/molecules17089002
Yan G, Ji L, Luo Y, Hu Y. Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography. Molecules. 2012; 17(8):9002-9009. https://doi.org/10.3390/molecules17089002
Chicago/Turabian StyleYan, Guilong, Lilian Ji, Yuming Luo, and Yonghong Hu. 2012. "Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography" Molecules 17, no. 8: 9002-9009. https://doi.org/10.3390/molecules17089002