LYRM1, a Gene that Promotes Proliferation and Inhibits Apoptosis during Heart Development
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effects of LYRM1 on cell differentiation
2.2. Effect of LYRM1 on cell proliferation
2.3. Effect of LYRM1 on cell apoptosis
2.4. Discussion
3. Experimental
3.1. Cell culture and differentiation of P19 cells
3.2. Quantitative real-time RT-PCR
Gene name | Product size | Reverse and forward primers (5'-3') |
GATA4 | 136bp | F:5'-CCTGCGGCCTCTACATGA-3' |
R:5'-AGGGTCTCACCAGCAGGA-3' | ||
Nkx2-5 | 222bp | F:5'-CCTGCGGCCTCTACATGA-3' |
R:5'-AGGGTCTCACCAGCAGGA-3' | ||
GAPDH | 237bp | F:5'-TTCACCACCATGGAGAAGGC-3' |
R:5'-GGCATGGACTGTGGTCATGA-3' |
3.3. Establishment of a stable cell line overexpressing LYRM1
3.4. MTT assay
3.5. Cell cycle assay
3.6. Apoptosis assay
3.7. Measurement of caspase-3 activity
3.8. Statistical analysis
4. Conclusions
Acknowledgements
- Sample Availability: Contact the authors.
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Zhu, C.; Liu, Y.-Q.; Chen, F.-K.; Hu, D.-L.; Yu, Z.-B.; Qian, L.-M. LYRM1, a Gene that Promotes Proliferation and Inhibits Apoptosis during Heart Development. Molecules 2010, 15, 6974-6982. https://doi.org/10.3390/molecules15106974
Zhu C, Liu Y-Q, Chen F-K, Hu D-L, Yu Z-B, Qian L-M. LYRM1, a Gene that Promotes Proliferation and Inhibits Apoptosis during Heart Development. Molecules. 2010; 15(10):6974-6982. https://doi.org/10.3390/molecules15106974
Chicago/Turabian StyleZhu, Chun, Yao-Qiu Liu, Fu-Kun Chen, De-Liang Hu, Zhang-Bin Yu, and Ling-Mei Qian. 2010. "LYRM1, a Gene that Promotes Proliferation and Inhibits Apoptosis during Heart Development" Molecules 15, no. 10: 6974-6982. https://doi.org/10.3390/molecules15106974