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In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs)

1
Centre for Drug Research, Universiti Sains Malaysia, 11800 USM Penang, Malaysia
2
School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Molecules 2010, 15(1), 432-441; https://doi.org/10.3390/molecules15010432
Received: 30 November 2009 / Revised: 2 January 2010 / Accepted: 7 January 2010 / Published: 20 January 2010
In the present study, we investigate the effects of three different Mitragyna speciosa extracts, namely methanolic, aqueous and total alkaloid extracts, on glutathione transferase-specific activity in male Sprague Dawley rat liver cytosol in vitro and in vivo. In the in vitro study, the effect of Mitragyna speciosa extracts (0.01 to 750 µg/mL) against the specific activity of glutathione transferases was examined in rat liver cytosolic fraction from untreated rats. Our data show concentration dependent inhibition of cytosolic GSTs when Mitragyna speciosa extract was added into the reaction mixture. At the highest concentration used, the methanolic extract showed the highest GSTs specific activity inhibition (61%), followed by aqueous (50%) and total alkaloid extract (43%), respectively. In in vivo study, three different dosages; 50, 100 and 200 mg/kg for methanolic and aqueous extracts and 5, 10 and 20 mg/kg for total alkaloid extract were given orally for 14 days. An increase in GST specific activity was generally observed. However, only Mitragyna speciosa aqueous extract with a dosage of 100 mg/kg showed significant results: 129% compared to control. View Full-Text
Keywords: herb-drug interactions; glutathione transferases; Mitragyna speciosa; in vitro; in vivo herb-drug interactions; glutathione transferases; Mitragyna speciosa; in vitro; in vivo
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MDPI and ACS Style

Azizi, J.; Ismail, S.; Mordi, M.N.; Ramanathan, S.; Said, M.I.M.; Mansor, S.M. In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs). Molecules 2010, 15, 432-441. https://doi.org/10.3390/molecules15010432

AMA Style

Azizi J, Ismail S, Mordi MN, Ramanathan S, Said MIM, Mansor SM. In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs). Molecules. 2010; 15(1):432-441. https://doi.org/10.3390/molecules15010432

Chicago/Turabian Style

Azizi, Juzaili, Sabariah Ismail, Mohd Nizam Mordi, Surash Ramanathan, Mohd Ikram Mohd Said, and Sharif Mahsufi Mansor. 2010. "In Vitro and in Vivo Effects of Three Different Mitragyna speciosa Korth Leaf Extracts on Phase II Drug Metabolizing Enzymes—Glutathione Transferases (GSTs)" Molecules 15, no. 1: 432-441. https://doi.org/10.3390/molecules15010432

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