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(1) Background: Sea fish with a high level of omega-3 very long-chain (VLC) PUFAs makes them a valuable component of a healthy diet. One of the most popular fish products is fish fingers, for which the market is still growing. The content of omega-3 VLC PUFAs in fish fingers may differ across price categories, such as premium or budget, despite being manufactured from the same fish species. Furthermore, the labelling of fish fingers typically fails to indicate the quantity of omega-3 VLC PUFAs present. It is unclear whether fish fingers can increase the amount of omega-3 VLC PUFAs in the diet. Hence, this study aimed to examine the content of omega-3 VLC PUFAs in pollock fish fingers from premium and budget price categories manufactured by the same producer and sold in supermarkets. (2) Methods: The premium fish fingers were made with pollock fillet and the budget ones with minced pollock meat. Fat content and fatty acids profile were analysed. (3) Results: The primary fatty acid found in fish fingers was oleic acid, followed by linoleic acid. Saturated fatty acids were less than 7%. This reflected the composition of frying fat, which was rapeseed oil. Fish fingers contained a dietary noticeable amount of omega-3 VLC PUFAs; for premium and budget fish fingers it was 283.01 mg and 123.44 mg per 100 g of product, respectively. (4) Conclusions: Not all fish fingers have the same nutritional value, despite being manufactured by the same producer and from the same fish species. Even though both were made from pollock, premium fish fingers were a better dietary source omega-3 EPA and DHA than a low-cost alternative. Eating three sticks (100 g) of examined pollock fish fingers, either premium or budget, can meet the recommended daily intake level of omega-3 VLC PUFAs at 100% or 50%, respectively. Full article

Sixteen novel circular rep-encoding DNA sequences with high sequence homologies to previously described SPHINX and BMMF sequences were isolated for the first time from non-bovine foods (pork, wild boar, chicken meat, Alaska pollock, pangasius, black tiger shrimp, apple, carrot, and sprouts from alfalfa, radish, and broccoli). The phylogenetic analysis of the full-length circular genomes grouped these together with previously described representatives of SPHINX/BMMF group 1 and 2 sequences (eight in each group). The characterization of genome lengths, genes present, and conserved structures confirmed their relationship to the known SPHINX/BMMF sequences. Further analysis of iteron-like tandem repeats of SPHINX/BMMF group 1-related genomes revealed a correlation with both full-length sequence tree branches as well as Rep protein sequence tree branches and was able to differentiate subtypes of SPHINX/BMMF group 1 members. For the SPHINX/BMMF group 2 members, a distinct grouping of sequences into two clades (A and B) with subgroups could be detected. A deeper investigation of potential functional regions upstream of the rep gene of the new SPHINX/BMMF group 2 sequences revealed homologies to the dso and sso regions of known plasmid groups that replicate via the rolling circle mechanism. Phylogenetic analyses were accomplished by a Rep protein sequence analysis of different ssDNA viruses, pCRESS, and plasmids with the known replication mechanism, as this yielded deeper insights into the relationship of SPHINX/BMMF group 1 and 2 Rep proteins. A clear relation of these proteins to the Rep proteins of plasmids could be confirmed. Interestingly, for SPHINX/BMMF group 2 members, the relationship to rolling circle replication plasmids could also be verified. Furthermore, a relationship of SPHINX/BMMF group 1 Rep proteins to theta-replicating plasmid Reps is discussed. Full article