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Keywords = vesicle, synthetic biology, fatty acid

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11 pages, 2387 KiB  
Article
Effect of the Membrane Composition of Giant Unilamellar Vesicles on Their Budding Probability: A Trade-Off between Elasticity and Preferred Area Difference
by Ylenia Miele, Gábor Holló, István Lagzi and Federico Rossi
Life 2021, 11(7), 634; https://doi.org/10.3390/life11070634 - 29 Jun 2021
Cited by 5 | Viewed by 3065
Abstract
The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges [...] Read more.
The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles. Full article
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9 pages, 101 KiB  
Review
Model Protocells from Single-Chain Lipids
by Sheref S. Mansy
Int. J. Mol. Sci. 2009, 10(3), 835-843; https://doi.org/10.3390/ijms10030835 - 2 Mar 2009
Cited by 44 | Viewed by 15228
Abstract
Significant progress has been made in the construction of laboratory models of protocells. Most frequently the developed vesicle systems utilize single-chain lipids rather than the double-chain lipids typically found in biological membranes. Although single-chain lipids yield less robust vesicles, their dynamic characteristics are [...] Read more.
Significant progress has been made in the construction of laboratory models of protocells. Most frequently the developed vesicle systems utilize single-chain lipids rather than the double-chain lipids typically found in biological membranes. Although single-chain lipids yield less robust vesicles, their dynamic characteristics are highly exploitable for protocellular functions. Herein the advantages of using single-chain lipids in the construction of protocells are discussed. Full article
(This article belongs to the Special Issue Origin of Life)
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