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The COVID-19 pandemic has exposed the extent of global connectivity and collective vulnerability to emerging diseases. From its suspected origins in Wuhan, China, it spread to all corners of the world in a matter of months. The absence of high-performance, rapid diagnostic methods that could identify asymptomatic carriers contributed to its worldwide transmission. Serological tests offer numerous benefits compared to other assay platforms to screen large populations. First-generation assays contain targets that represent proteins from SARS-CoV-2. While they could be quickly produced, each actually has a mixture of specific and non-specific epitopes that vary in their reactivity for antibodies. To generate the next generation of the assay, epitopes were identified in three SARS-Cov-2 proteins (S, N, and Orf3a) by SPOT synthesis analysis. After their similarity to other pathogen sequences was analyzed, 11 epitopes outside of the receptor-binding domain (RBD) of the spike protein that showed high reactivity and uniqueness to the virus. These were incorporated into a ß-barrel protein core to create a highly chimeric protein. Another de novo protein was designed that contained only epitopes in the RBD. In-house ELISAs suggest that both multiepitope proteins can serve as targets for high-performance diagnostic tests. Our approach to bioengineer chimeric proteins is highly amenable to other pathogens and immunological uses. Full article

In The Souls of Black Folk, W.E.B. DuBois discusses the historical and cultural beginnings of the black preacher as “the most unique personality developed on American soil.” He writes, “[the black preacher] found his functions as the healer of the sick, the interpreter of the Unknown, the comforter of the sorrowing, the supernatural avenger of wrong…Thus as bard, physician, judge, and priest within the narrow limits allowed by the slave system rose the Negro preacher.” Far from being a monolith, the preacher figure embodies many complexities and variances on how the preached Word can be delivered. This begs the question, in what ways can we reimagine DuBois’s black preacher figure in his words, “the most unique personality developed on American soil,” as a black woman? What remains to be seen in scholarship of the mid-twentieth century is an articulation of the black woman preacher in African American literature. By reimagining and refiguring a response to DuBois’s assertion above, how is the role of the black woman preacher and impact of her sermons portrayed in African American literature? Using the art of the sermon, the intersection of music, and James Baldwin’s The Amen Corner as a central text, this article examines the black woman preacher in character and African American women’s spirituality in twentieth century literature. I argue that the way in which Margaret Alexander, as a black woman preacher in the text, creates sermonic spaces of healing and restoration (exegetically and eschatologically) for herself and others outside of the church becomes a new mode of social and cultural resistance. This article works to re-envision the black woman and reposition her in the center of religious discourse on our way to unearthing the modes of transfiguration black women preachers evoke in and out of the pulpit. Full article