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Keywords = tea plant necrotic ring blotch virus

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9 pages, 1699 KiB  
Communication
The Occurrence and Genetic Variability of Tea Plant Necrotic Ring Blotch Virus in Fujian Province, China
by Xihong Chen, Jianguo Shen, Min Li, Yujie Gao, Zhenguo Du and Fangluan Gao
Forests 2023, 14(9), 1755; https://doi.org/10.3390/f14091755 - 30 Aug 2023
Viewed by 1521
Abstract
Tea plant necrotic ring blotch virus (TPNRBV) is a kitavirus that poses a serious threat to the cultivation of tea, one of the most economically important plants in many Asian countries. However, the occurrence and genetic variability of this virus remain poorly understood. [...] Read more.
Tea plant necrotic ring blotch virus (TPNRBV) is a kitavirus that poses a serious threat to the cultivation of tea, one of the most economically important plants in many Asian countries. However, the occurrence and genetic variability of this virus remain poorly understood. This study investigated the occurrence of TPNRBV in Fujian Province, China, one of the largest tea producers in the country, and determined the complete genome of 10 new TPNRBV isolates. The results revealed that TPNRBV is widespread in Fujian. The nucleotide diversity values for the RNA1-4 segments of TPNRBV were found to be 0.027, 0.016, 0.027, and 0.014, respectively. Among the seven proteins of TPNRBV, P22 was the least conserved, while MP was the most conserved. The 5′ termini of the genomic segments of TPNRBV commonly possessed a sequence of 5′-AATTACGA-3′ (RNA1-3) or 5′-ATTAACGA-3′ (RNA4). Furthermore, the 3′ non-coding region of TPNRBV RNA4 exhibited significant length variation due to frequent deletion/insertion mutations. Recombination and purifying selection likely played important roles in shaping the genetic structure of TPNRBV. These findings provide a snapshot of the epidemiology and genetic structure of TPNRBV, offering valuable information for the development of targeted strategies to control and manage TPNRBV in tea cultivation. Full article
(This article belongs to the Section Forest Health)
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11 pages, 2181 KiB  
Article
Quantitative Distribution and Transmission of Tea Plant Necrotic Ring Blotch Virus in Camellia sinensis
by Hengze Ren, Yao Chen, Fumei Zhao, Changqing Ding, Kexin Zhang, Lu Wang, Yajun Yang, Xinyuan Hao and Xinchao Wang
Forests 2022, 13(8), 1306; https://doi.org/10.3390/f13081306 - 16 Aug 2022
Cited by 8 | Viewed by 2678
Abstract
Tea plant necrotic ring blotch virus (TPNRBV), which carries four positive-sense single-stranded RNA segments, causes discoloration spots and multiple necrotic ring blotches in tea trees. To understand the distribution and transmission of TPNRBV in tea trees and prevent its spread, a SYBR Green [...] Read more.
Tea plant necrotic ring blotch virus (TPNRBV), which carries four positive-sense single-stranded RNA segments, causes discoloration spots and multiple necrotic ring blotches in tea trees. To understand the distribution and transmission of TPNRBV in tea trees and prevent its spread, a SYBR Green real-time quantitative polymerase chain reaction (RT-qPCR) method for detecting the four virus segments was developed. The limit of detection of RT-qPCR was 3.81, 4.73, 3.58, and 4.64 copies/μL for the four strands of TPNRBV, which was 100-fold more sensitive than conventional PCR for RNA1 detection, 10-fold for RNA2 and RNA3, and 1000-fold for RNA4 detection. Visual observation and RT-qPCR of different tea plant tissues showed that symptomatic mature leaves contained the highest TPNRBV load; the concentrations of the four RNAs in other tissues decreased or were undetectable with increasing distances from symptomatic leaves. TPNRBV did not spread efficiently through seeds, cuttings, or mechanical inoculation, but was transmitted to some tea cultivars, particularly light albinistic varieties such as ‘Zhonghuang 1’ and ‘Huangjinya’, under field conditions. Our TPNRBV detection method is useful for determining the distribution and transmission characteristics of TPNRBV and selecting tissues with the highest viral load for early diagnosis, monitoring, and management of the disease. Full article
(This article belongs to the Special Issue Viruses in Forest and Urban Trees and Shrubs)
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