During our systematic study on the anticancer activities of
Scutellaria barbata, scutebarbatine A (SBT-A), one of the major alkaloids in
S. barbata, was found to have antitumor effects on A549 cells. Thus, we designed the present study to investigate in detail
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During our systematic study on the anticancer activities of
Scutellaria barbata, scutebarbatine A (SBT-A), one of the major alkaloids in
S. barbata, was found to have antitumor effects on A549 cells. Thus, we designed the present study to investigate in detail the antitumor effects of SBT-A. The cytotoxic effect of SBT-A on A549
in vitro were determined by an MTT assay and evaluated by IC
50 values. Furthermore, results of Hoechst 33258 and Annexin V/PI staining assays demonstrated that SBT-A had significant antitumor effects on A549 cells via apoptosis, in a concentration-dependent manner. What’s more, the mechanism was explored by western blotting, and our study revealed that SBT-A can up-regulate the expressions of cytochrome c, caspase-3 and 9, and down-regulate the levels of Bcl-2 in A549 cells. Finally, the antitumor effects of SBT-A were evaluated
in vivo by using transplanted tumor nude mice, and the results confirmed that SBT-A has a notable antitumor effect on A549 cancer via mitochondria-mediated apoptosis. Collectively, our results demonstrated that SBT-A showed significant antitumor effects on A549 cells
in vivo and
in vitro via mitochondria-mediated apoptosis by up-regulating expressions of caspase-3 and 9, and down-regulating Bcl-2.
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