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10 pages, 779 KiB  
Article
Intake of a Mixture of Sake Cake and Rice Malt Increases Mucin Levels and Changes in Intestinal Microbiota in Mice
by Shinpei Kawakami, Ryouichi Ito, Hiroko Maruki-Uchida, Asuka Kamei, Akihito Yasuoka, Tsudoi Toyoda, Tomoko Ishijima, Eisaku Nishimura, Minoru Morita, Masahiko Sai, Keiko Abe and Shinji Okada
Nutrients 2020, 12(2), 449; https://doi.org/10.3390/nu12020449 - 11 Feb 2020
Cited by 15 | Viewed by 4827
Abstract
Amazake is a traditional Japanese beverage. Its main ingredients are sake cake and rice malt. In this study, we examined the effect of sake cake and rice malt on the intestinal barrier function and gut microbiota. BALB/c mice were fed a control diet [...] Read more.
Amazake is a traditional Japanese beverage. Its main ingredients are sake cake and rice malt. In this study, we examined the effect of sake cake and rice malt on the intestinal barrier function and gut microbiota. BALB/c mice were fed a control diet or a diet containing a mixture of sake cake and rice malt powder (SRP) for four weeks. Fecal IgA values did not change between groups, but the fecal mucin level was significantly greater in the SRP-fed group. Gene expression analysis in the ileum by real-time PCR demonstrated Muc2 expression did not change, while the Muc3 expression was upregulated in the SRP-fed group. Furthermore, microbiota analysis demonstrated a change by SRP intake at the family level, and the proportion of Lactobacillaceae significantly increased in the SRP-fed group. At the genus level, the proportion of Lactobacillus also significantly increased in the SRP-fed group. These results suggest that the intake of a mixture of sake cake and rice malt improves intestinal barrier function by increasing mucin levels and inducing changes in intestinal microbiota. Full article
(This article belongs to the Special Issue Impact of Dietary Components on Gut Microbiota)
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