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Keywords = precolostral serum

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12 pages, 969 KB  
Article
Evaluation of the Differences in the Serum Protein Electrophoretic Pattern in Precolostral Serum of Farm Animal Neonates
by Csilla Tóthová, Róbert Link, Veronika Glembová and Oskar Nagy
Agriculture 2023, 13(5), 1035; https://doi.org/10.3390/agriculture13051035 - 10 May 2023
Cited by 2 | Viewed by 2610
Abstract
The objective of this study was to compare the electrophoretic pattern of serum proteins in newborn calves, lambs, goat kids and piglets in precolostral blood samples. Blood samples were collected within 30 min of birth, prior to ingestion of first colostrum, and the [...] Read more.
The objective of this study was to compare the electrophoretic pattern of serum proteins in newborn calves, lambs, goat kids and piglets in precolostral blood samples. Blood samples were collected within 30 min of birth, prior to ingestion of first colostrum, and the concentrations of total proteins and protein fractions were analyzed using electrophoresis on agarose gel. The size and shape of the protein fractions on the electrophoretograms differed among the investigated animal species. Significant differences were found in the total protein values and all the separated protein fractions, as well as albumin to globulin ratio (p < 0.01 and p < 0.001). The mean total protein concentration in piglets was lower compared to ruminants. Albumin concentrations were markedly higher, and the concentrations of α1-globulins were lower, in neonatal ruminants compared to piglets. The values of α2-globulins were higher in lambs and goat kids, and lower in calves and piglets. An opposite tendency was found in the values of β-globulins: a detectable amount of γ-globulins was recorded in all evaluated animal species. Presented results suggest marked species related differences in the shape and size of protein fraction among neonates of farm animal species, and the importance of the evaluation of electrophoretograms with regard to these findings. Full article
(This article belongs to the Special Issue Welfare, Behavior and Health of Farm Animals)
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6 pages, 2310 KB  
Communication
Optimizing the Measurement of Colostrum Antibody Concentrations for Identifying BVDV Persistently Infected Calves
by Caitlin J. Jenvey, Michael P. Reichel, Sasha R. Lanyon and Peter D. Cockcroft
Vet. Sci. 2015, 2(1), 26-31; https://doi.org/10.3390/vetsci2010026 - 9 Mar 2015
Cited by 2 | Viewed by 5880
Abstract
Colostrum contains substantially higher concentrations of immunoglobulins compared to serum, which may help to improve the utility of diagnostic tests. The aim of this study was to determine the diagnostic value of colostrum antibody concentrations in identifying Bovine Viral Diarrhoea Virus (BVDV) PI [...] Read more.
Colostrum contains substantially higher concentrations of immunoglobulins compared to serum, which may help to improve the utility of diagnostic tests. The aim of this study was to determine the diagnostic value of colostrum antibody concentrations in identifying Bovine Viral Diarrhoea Virus (BVDV) PI (persistently infected) calf carrying beef heifers following an experimental infection. Colostrum was collected within 12 hours of parturition and tested in undiluted, 1:5, 1:10, 1:100, 1:200, and 1:500 dilutions using an enzyme-linked immunosorbent assay (ELISA) for BVDV antibody. Cows were determined to be carrying a PI calf based on positive quantitative Real Time-Polymerase Chain Reaction and antigen ELISA result on pre-colostral serum and ear notch samples collected from their calf. The median ELISA sample-to-positive (S/P) ratio for colostrum collected from heifers that carried a PI calf were significantly higher than the median ELISA S/P ratio for colostrum collected from heifers that did not carry a PI calf at dilutions of 1:100, 1:200, and 1:500. This study provides further evidence for increased antigenic stimulation in utero by the BVDV viraemic PI calf, which can also be identified with 100% diagnostic sensitivity when using 1:500 dilution colostrum. Full article
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