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Article

1,939 Views

13 Pages

Escherichia coli Reporter Strains Allow for the In Vivo Evaluation of Recombinant Elongation Factor Protein (EF-P)

Natalia Trachtmann,
Aydar Bikmullin,
Shamil Validov and
Georg A. Sprenger

Appl. Microbiol.2024, 4(3), 1335-1347;https://doi.org/10.3390/applmicrobiol4030092

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18 September 2024

Background: Elongation factor protein (EF-P) in bacteria helps ribosomes to incorporate contiguous proline residues (xPro) into proteins. In this way, EF-P rescues ribosomes from stalling at these xPro motifs. Whereas EF-P deficiency is lethal for so...

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Article

19 Citations

5,031 Views

23 Pages

Sequence-Dependent Correlated Segments in the Intrinsically Disordered Region of ChiZ

Alan Hicks,
Cristian A. Escobar,
Timothy A. Cross and
Huan-Xiang Zhou

Biomolecules2020, 10(6), 946;https://doi.org/10.3390/biom10060946

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23 June 2020

How sequences of intrinsically disordered proteins (IDPs) code for their conformational dynamics is poorly understood. Here, we combined NMR spectroscopy, small-angle X-ray scattering (SAXS), and molecular dynamics (MD) simulations to characterize th...

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