Polyhydroxyalkanoates (PHAs), synthesized by
Xanthomonas to endure adverse conditions, are primarily regulated by the critical genes
phaC and
phaZ. Poly-3-hydroxybutyrate (PHB), a common polyhydroxyalkanoate (PHA), has been implicated in metabolism, pathogenicity, and various physiological processes in
Xanthomonas oryzae pv.
oryzae (
Xoo
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Polyhydroxyalkanoates (PHAs), synthesized by
Xanthomonas to endure adverse conditions, are primarily regulated by the critical genes
phaC and
phaZ. Poly-3-hydroxybutyrate (PHB), a common polyhydroxyalkanoate (PHA), has been implicated in metabolism, pathogenicity, and various physiological processes in
Xanthomonas oryzae pv.
oryzae (
Xoo). In this study, we investigated the effects of HN-2 using n-butanol extract (HN-2 n-butanol extract) derived from
Bacillus velezensis on
Xoo. The results showed that HN-2 n-butanol extract could induce PHB accumulation in
Xoo, potentially via surfactin. Moreover, examination of drug resistance, pathogenicity, and morphological characteristics of
Xoo revealed PHB played a significant role in the drug resistance, pathogenicity, membrane integrity, and growth rate of
Xoo strains following the deletion of
phaZ and
phaC. The ∆
phaZ strain was the most significant, with a growth rate reduced to 58.19% of the
PXO99A at 36 h and an inhibition zone 57.46% larger than that of
PXO99A by HN-2 n-butanol extract. Transmission electron microscopy further revealed blank spots in
Xoo after treatment, with the fewest spots observed in ∆
phaZ, indicating its impaired ability to repair and maintain membrane integrity. These findings offer valuable insights that could serve as a foundation for elucidating the mechanisms of drug resistance and future research on preventing
Xoo-induced diseases.
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