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Keywords = paenibacillus polymyxa ZF129

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17 pages, 6199 KB  
Article
Coating Seeds with Paenibacillus polymyxa ZF129 Microcapsule Suspension Enhanced Control Effect on Fusarium Root Rot and Promoted Seedling Growth in Cucumber
by Jiayi Ma, Jialin Liu, Yanxia Shi, Xuewen Xie, Ali Chai, Sheng Xiang, Xianhua Sun, Lei Li, Baoju Li and Tengfei Fan
Biology 2025, 14(4), 375; https://doi.org/10.3390/biology14040375 - 5 Apr 2025
Cited by 3 | Viewed by 1029
Abstract
Fusarium root rot, a destructive soil-borne fungal disease, necessitates eco-friendly biocontrol strategies. This study developed a microbial seed-coating approach using the antagonistic strain Paenibacillus polymyxa ZF129, formulated into a microencapsulated powder (108 CFU/g) and a suspension (CS-ZF129). CS-ZF129 application enhanced cucumber resistance, [...] Read more.
Fusarium root rot, a destructive soil-borne fungal disease, necessitates eco-friendly biocontrol strategies. This study developed a microbial seed-coating approach using the antagonistic strain Paenibacillus polymyxa ZF129, formulated into a microencapsulated powder (108 CFU/g) and a suspension (CS-ZF129). CS-ZF129 application enhanced cucumber resistance, achieving 46.30 ± 0.02% disease suppression while promoting root growth. The maximum increase in the fresh weight of the root in the promotion of rectangular growth was 47.16%. The colonization dynamics of ZF129 in the rhizosphere were systematically tracked, revealing its antagonistic correlation with Fusarium proliferation. An enzymatic activity analysis further uncovered the underlying regulatory mechanisms, demonstrating induced defense responses through pathogenesis-related protein activation. These findings highlight ZF129’s dual functionality as a biocontrol agent and a plant growth promoter, offering a sustainable strategy against soil-borne pathogens. Full article
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14 pages, 3541 KB  
Article
Seed Pelleting with Gum Arabic-Encapsulated Biocontrol Bacteria for Effective Control of Clubroot Disease in Pak Choi
by Rizwangul Abdukerim, Sheng Xiang, Yanxia Shi, Xuewen Xie, Lei Li, Ali Chai, Baoju Li and Tengfei Fan
Plants 2023, 12(21), 3702; https://doi.org/10.3390/plants12213702 - 27 Oct 2023
Cited by 10 | Viewed by 3122
Abstract
Clubroot is one of the most serious soil-borne diseases on crucifer crops worldwide. Seed treatment with biocontrol agents is an effective and eco-friendly way to control clubroot disease. However, there is a big challenge to inoculating the seed with bacterial cells through seed [...] Read more.
Clubroot is one of the most serious soil-borne diseases on crucifer crops worldwide. Seed treatment with biocontrol agents is an effective and eco-friendly way to control clubroot disease. However, there is a big challenge to inoculating the seed with bacterial cells through seed pelleting due to the harsh environment on the seed surface or in the rhizosphere. In this study, a method for microbial seed pelleting was developed to protect pak choi seedlings against clubroot disease. Typically, a biocontrol bacterium, Paenibacillus polymyxa ZF129, was encapsulated by the spray-drying method with gum arabic as wall material, and then pak choi seeds were pelleted with the microencapsulated Paenibacillus polymyxa ZF129 (ZF129m). The morphology, storage stability, and release behavior of ZF129 microcapsules were evaluated. Compared with the naked Paenibacillus polymyxa ZF129 cells, encapsulated ZF129 cells showed higher viability during ambient storage on pak choi seeds. Moreover, ZF129m-pelleted seeds showed higher control efficacy (71.23%) against clubroot disease than that of nonencapsulated ZF129-pelleted seeds (61.64%) in pak choi. Seed pelleting with microencapsulated biocontrol Paenibacillus polymyxa ZF129 proved to be an effective and eco-friendly strategy for the control of clubroot disease in pak choi. Full article
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